Mouse CCR6 Antibody Summary
Met1-Met367
Accession # O54689
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) or (B) Rat IgG2A control antibody (Catalog # MAB006) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F). View our protocol for Staining Membrane-associated Proteins.
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CCR6 in Mouse Thymus. CCR6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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CCR6 in Mouse Spleen. CCR6 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Mouse CCR6 by Flow Cytometry Priming of pik3cg−/− CD4+ T cells is reduced during EAE.(A) Frequencies of CD4+ cells in draining LN that are CD69+ on day 9 post-immunisation for EAE as determined by flow cytometry. A representative histogram overlay gating on CD4+ cells is shown (filled = isotype control on WT, solid line = anti-CD69 on WT, dotted line = anti-CD69 on pik3cg−/−) (B) In vivo proliferation of CD4+ cells, measured by BrdU incorporation, is reduced in pik3cg−/− mice at day 9 post-immunization for EAE. (n = 6 mice per group). Representative dot plots gating on CD4+ cells are shown. (C) Expression of chemokine receptors by CD4+ T cells indicative of T cell activation was determined by flow cytometry. Representative histogram overlays showing expression of CCR7, CCR6 and CXCR3 are shown (filled = isotype control, solid line = WT, dotted line = pik3cg−/−). (D) Expression of CD62L, CD49d and PSGL-1 by CD4+ cells from spleen of day 9 immunised mice (n = 3 mice per group). All data shown are mean ± s.e.m. (*, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0045095), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR6
CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.
Product Datasheets
Citations for Mouse CCR6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Sciatic–Vagal Nerve Stimulation by Electroacupuncture Alleviates Inflammatory Arthritis in Lyme Disease-Susceptible C3H Mice
Authors: Lavoisier Akoolo, Vitomir Djokic, Sandra C. Rocha, Luis Ulloa, Nikhat Parveen
Frontiers in Immunology
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IL-1? and IL-23 Promote Extrathymic Commitment of CD27(+)CD122(-) ?? T Cells to ??T17 Cells
Authors: A Muschaweck, F Petermann, T Korn
J. Immunol., 2017-08-30;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
J. Immunol, 2016-02-05;196(6):2514-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages.
Authors: Boyle S, Faulkner J, McColl S, Kochetkova M
Mol Cancer, 2015-06-06;14(0):115.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.
Authors: Liu, Jinlin, Ke, Fang, Xu, Zhenyao, Liu, Zhaoyuan, Zhang, Lingyun, Yan, Sha, Wang, Zhe, Wang, Hong, Wang, Honglin
PLoS ONE, 2014-06-30;9(6):e101137.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
SerpinB1 regulates homeostatic expansion of IL-17+ gammadelta and CD4+ Th17 cells.
Authors: Zhao P, Hou L, Farley K, Sundrud M, Remold-O'Donnell E
J Leukoc Biol, 2013-11-18;95(3):521-30.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis
Authors: Pedro Caballero-Campo, Mariano G. Buffone, Fabian Benencia, José R. Conejo-García, Paolo F. Rinaudo, George L. Gerton
Journal of Cellular Physiology
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Programmed downregulation of CCR6 is important for establishment of epidermal gammadeltaT cells by regulating their thymic egress and epidermal location.
Authors: Hu S, Xiong N
J Immunol, 2013-02-18;190(7):3267-75.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Colonic patch and colonic SILT development are independent and differentially regulated events.
Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
Mucosal Immunol, 2012-09-19;6(3):511-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
PI3K gamma drives priming and survival of autoreactive CD4(+) T cells during experimental autoimmune encephalomyelitis
Authors: Iain Comerford, Wendel Litchfield, Ervin Kara, Shaun R. McColl
PLoS ONE
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CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
J Invest Dermatol, 2012-08-16;133(1):164-71.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Tumor-Associated Macrophages Recruit CCR6+ Regulatory T Cells and Promote the Development of Colorectal Cancer via Enhancing CCL20 Production in Mice
Authors: Jinlin Liu, Ning Zhang, Qun Li, Weiwei Zhang, Fang Ke, Qibin Leng et al.
PLoS ONE
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Human beta-defensin 2 and 3 and their mouse orthologs induce chemotaxis through interaction with CCR2.
Authors: Rohrl J, Yang D, Oppenheim JJ, Hehlgans T
J. Immunol., 2010-05-17;184(12):6688-94.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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murine splenic CD4 T cells were stained.
