Mouse CD11c Antibody

Catalog #: MAB6950
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6950
MAB6950-SP

Detection of Mouse CD11c by Western Blot.

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All CD11c Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD11c Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD11c in direct ELISAs and Western blots. In direct ELISAs, 50%‑100% cross‑reactivity with recombinant human (rh) Integrin alpha X beta 2 and recombinant mouse (rm) Integrin alpha L is observed, 10%‑25% cross‑reactivity with rhIntegrin CD11c, rhIntegrin alpha D beta 2, and rhIntegrin alpha L beta 2 is observed and no cross-reactivity with rhIntegrin alpha M or rhIntegrin beta 2 is observed.

Source

Monoclonal Rat IgG_2B Clone # 435421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD11c
Phe20-Pro1116
Accession # Q9QXH4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of mouse lung

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD11c by Western Blot. Western blot shows lysates of mouse lymph node tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB6950) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD11c at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Detection of CD11c in Mouse Lung. CD11c was detected in immersion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB6950) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to macrophages. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11c

CD11c, also known as the Integrin alpha X subunit, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some other leukocyte subsets. The 1097 aa mouse CD11c extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat CD11c, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b/CD18 (Complement iC3b, ICAMs, vWF and Fibrinogen) while others (Osteopontin, Thy-1, Plasminogen, Heparin) are unique. Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.

Entrez Gene IDs

3687 (Human); 16411 (Mouse); 64350 (Rat)

Alternate Names

95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6

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Related Research Areas

Citations for Mouse CD11c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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