Mouse DLL4 Antibody

Catalog #: MAB13891 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13891
MAB13891-SP

All DLL4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DLL4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DLL4 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human DLL4, recombinant mouse (rm) DLL1, rmNotch-1, rmNotch-2, or rmNotch-3 is observed.

Source

Monoclonal Rat IgG_2A Clone # 207811

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DLL4
Ser28-Pro525
Accession # NP_062327

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Mouse DLL4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse DLL4 Biotinylated Antibody (Catalog # BAM13892)

Standard: Recombinant Mouse DLL4 His-tag Protein (Catalog # 1389-D4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2‑4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

References

Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
Walker, L. et al. (2001) Stem Cells 19:543.
Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

Long Name

Delta-like 4

Entrez Gene IDs

54567 (Human); 54485 (Mouse); 100152163 (Porcine)

Alternate Names

Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4