Mouse DNER Antibody

Catalog # Availability Size / Price Qty
MAB2254-SP Ships in 5-7 Days25 ug / $149
MAB2254 In Stock100 ug / $399

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Mouse DNER Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DNER is observed.
Source
Monoclonal Rat IgG1 Clone # 298306
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DNER
Ala26-His637
Accession # Q8JZM4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse DNER under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNER

DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch.

Long Name
Delta/Notch-like EGF Repeat-containing Transmembrane
Entrez Gene IDs
92737 (Human); 227325 (Mouse)
Alternate Names
bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; DNER; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1

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