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Mouse DPPII/QPP/DPP7 Antibody

Catalog #: AF3436
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3436
AF3436-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DPPII/QPP/DPP7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DPPII/QPP/DPP7 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human DPPII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DPPII/QPP/DPP7
Asp34-Arg506
Accession # NP_114031
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse DPPII/QPP/DPP7 (Catalog # 3436-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Mouse DPPII/QPP/DPP7 (0.02 µg/mL, Catalog # 3436-SE) cleavage of the fluorogenic peptide substrate KP-Amc (10 µM). The Neutralization Dose (ND50) is typically 0.45 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPII/QPP/DPP7

Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have a high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The mouse DPP7 precursor consists of a  signal peptide (amino acids (aa) 1‑33) and a mature chain (aa 34‑506). The purified recombinant mouse DPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under otherwise identical conditions.

References
  1. Araki, H. et al. (2001) J. Biochem. 129:279.
  2. Maes, M.-B. et al. (2005) Biochem. J. 386:315.
  3. Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
  4. Lankas, G.R. et al. (2005) Diabetes 54:2988.
Long Name
Dipeptidyl-peptidase II
Entrez Gene IDs
29952 (Human); 83768 (Mouse); 83799 (Rat)
Alternate Names
carboxytripeptidase; Dipeptidyl aminopeptidase II; dipeptidyl arylamidase II; dipeptidyl peptidase 2; Dipeptidyl peptidase 7; Dipeptidyl peptidase II; dipeptidylpeptidase 7; dipeptidyl-peptidase 7; dipeptidyl-peptidase II; DPP II; DPP2; DPP7; DPPII; EC 3.4.14.2; QPP; Quiescent cell proline dipeptidase

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Citations for Mouse DPPII/QPP/DPP7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
    Authors: J Frew, HB Nygaard
    Acta neuropathologica communications, 2021-04-01;9(1):57.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  2. Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient Mice
    Authors: Zoe A. Klein, Hideyuki Takahashi, Mengxiao Ma, Massimiliano Stagi, Melissa Zhou, TuKiet T. Lam et al.
    Neuron

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