Home / DPPIV/CD26 / Mouse DPPIV/CD26 Antibody

Mouse DPPIV/CD26 Antibody

Catalog #: AF954
37 Citations 2 Reviews Datasheet / COA / SDS
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AF954
AF954-SP
Detection of Mouse and Rat DPPIV/CD26 by Western Blot.
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Product Details
Citations (37)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DPPIV/CD26 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than  20% cross‑reactivity with recombinant human DPPIV/CD26 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
Ser29-His760
Accession # P28843
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
.25 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat DPPIV/CD26 antibody by Western Blot. View Larger

Detection of Mouse and Rat DPPIV/CD26 by Western Blot. Western blot shows lysates of mouse thymus tissue and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DPPIV/CD26 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry DPPIV/CD26 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

DPPIV/CD26 in Mouse Thymus. DPPIV/CD26 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse and Rat DPPIV/CD26 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse and Rat DPPIV/CD26 by Simple WesternTM. Simple Western lane view shows lysates of rat lung tissue and mouse thymus tissue, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 144-169 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

References
  1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
  2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
  5. Vivier, I. et al. (1991) J. Immunol. 147:447.
  6. Callebaut, C. et al. (1993) Science 262:2045.
  7. Kameoka, J. et al. (1993) Science 261:466.
  8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.
Long Name
Dipeptidyl-peptidase IV
Entrez Gene IDs
1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)
Alternate Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

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Citations for Mouse DPPIV/CD26 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Lef1 expression in fibroblasts maintains developmental potential in adult skin to regenerate wounds
    Authors: Quan M Phan, Gracelyn M Fine, Lucia Salz, Gerardo G Herrera, Ben Wildman, Iwona M Driskell et al.
    eLife
  2. Loss of myosin Vb promotes apical bulk endocytosis in neonatal enterocytes
    Authors: Engevik AC, Kaji I, Postema MM et al.
    J. Cell Biol.
  3. Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth
    Authors: Krivanek J, Soldatov RA, Kastriti ME et al.
    Nature Communications
  4. Fibroblast state switching orchestrates dermal maturation and wound healing
    Authors: Emanuel Rognoni, Angela Oliveira Pisco, Toru Hiratsuka, Kalle H Sipilä, Julio M Belmonte, Seyedeh Atefeh Mobasseri et al.
    Molecular Systems Biology
  5. Dynamic Formation of Microvillus Inclusions During Enterocyte Differentiation in Munc18-2–Deficient Intestinal Organoids
    Authors: Mohammed H. Mosa, Ophélie Nicolle, Sophia Maschalidi, Fernando E. Sepulveda, Aurelien Bidaud-Meynard, Constantin Menche et al.
    Cellular and Molecular Gastroenterology and Hepatology
  6. The Dipeptidyl Peptidase-4 Inhibitor Linagliptin Directly Enhances the Contractile Recovery of Mouse Hearts at a Concentration Equivalent to that Achieved with Standard Dosing in Humans
    Authors: Sri Nagarjun Batchu, Veera Ganesh Yerra, Youan Liu, Suzanne L. Advani, Thomas Klein, Andrew Advani
    International Journal of Molecular Sciences
  7. 3D spherical microtissues and microfluidic technology for multi-tissue experiments and analysis
    Authors: Jin-Young Kim, David A. Fluri, Rosemarie Marchan, Kurt Boonen, Soumyaranjan Mohanty, Prateek Singh et al.
    Journal of Biotechnology
  8. TGF‐ beta 1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation
    Authors: Jung Eun Baik, Hyeung Ju Park, Raghu P. Kataru, Ira L. Savetsky, Catherine L. Ly, Jinyeon Shin et al.
    Clinical and Translational Medicine
  9. Charting human development using a multi-endodermal organ atlas and organoid models
    Authors: Qianhui Yu, Umut Kilik, Emily M. Holloway, Yu-Hwai Tsai, Christoph Harmel, Angeline Wu et al.
    Cell
  10. Pancreas-derived DPP4 is not essential for glucose homeostasis under metabolic stress
    Authors: Evgenia Fadzeyeva, Cassandra A.A. Locatelli, Natasha A. Trzaskalski, My-Anh Nguyen, Megan E. Capozzi, Branka Vulesevic et al.
    iScience
  11. Protocols for staining of bile canalicular and sinusoidal networks of human, mouse and pig livers, three-dimensional reconstruction and quantification of tissue microarchitecture by image processing and analysis
    Authors: Seddik Hammad, Stefan Hoehme, Adrian Friebel, Iris von Recklinghausen, Amnah Othman, Brigitte Begher-Tibbe et al.
    Archives of Toxicology
  12. Differential Anti-Tumor Effects of IFN-Inducible Chemokines CXCL9, CXCL10, and CXCL11 on a Mouse Squamous Cell Carcinoma Cell Line
    Authors: Ari Matsumoto, Miki Hiroi, Kazumasa Mori, Nobuharu Yamamoto, Yoshihiro Ohmori
    Medical Sciences
  13. Lineage tracing of Foxd1‐expressing embryonic progenitors to assess the role of divergent embryonic lineages on adult dermal fibroblast function
    Authors: John T. Walker, Lauren E. Flynn, Douglas W. Hamilton
    FASEB BioAdvances
  14. Loss of MYO5B in Mice Recapitulates Microvillus Inclusion Disease and Reveals an Apical Trafficking Pathway Distinct to Neonatal Duodenum
    Authors: Victoria G. Weis, Byron C. Knowles, Eunyoung Choi, Anna E. Goldstein, Janice A. Williams, Elizabeth H. Manning et al.
    Cellular and Molecular Gastroenterology and Hepatology
  15. Structural characterization of SLYM - a 4 th meningeal membrane
    Authors: Plá, V;Bitsika, S;Giannetto, M;Ladron-de-Guevara, A;Gahn-Martinez, D;Mori, Y;Nedergaard, M;Møllgård, K;
    bioRxiv : the preprint server for biology
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration
    Authors: Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ;
    Cell reports
  17. Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development
    Authors: Q Liu, C Li, B Deng, P Gao, L Wang, Y Li, M Shiri, F Alkaifi, J Zhao, JM Stephens, CA Simintiras, J Francis, J Sun, X Fu
    Cell Reports, 2023-02-28;42(3):112166.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  18. Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regeneration
    Authors: N Mahmud, C Eisner, S Purushotha, MA Storer, DR Kaplan, FD Miller
    Cell Reports, 2022-12-20;41(12):111853.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  19. Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation
    Authors: S Pantasis, J Friemel, SM Brütsch, Z Hu, S Krautbauer, G Liebisch, J Dengjel, A Weber, S Werner, MR Bordoli
    Journal of Cell Science, 2022-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Suspension culture promotes serosal mesothelial development in human intestinal organoids
    Authors: MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence
    Cell Reports, 2022-02-15;38(7):110379.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  21. Dpp4+ interstitial progenitor cells contribute to basal and high fat diet-induced adipogenesis
    Authors: M Stefkovich, S Traynor, L Cheng, D Merrick, P Seale
    Molecular Metabolism, 2021-10-15;0(0):101357.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Cyst formation in proximal renal tubules caused by dysfunction of the microtubule minus-end regulator CAMSAP3
    Authors: Y Mitsuhata, T Abe, K Misaki, Y Nakajima, K Kiriya, M Kawasaki, H Kiyonari, M Takeichi, M Toya, M Sato
    Scientific Reports, 2021-03-12;11(1):5857.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Pathologic HIF1&alpha signaling drives adipose progenitor dysfunction in obesity
    Authors: M Shao, C Hepler, Q Zhang, B Shan, L Vishvanath, GH Henry, S Zhao, YA An, Y Wu, DW Strand, RK Gupta
    Cell Stem Cell, 2021-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth
    Authors: Krivanek J, Soldatov RA, Kastriti ME et al.
    Nature Communications
  25. CD47 prevents the elimination of diseased fibroblasts in scleroderma
    Authors: T Lerbs, L Cui, ME King, T Chai, C Muscat, L Chung, R Brown, K Rieger, T Shibata, G Wernig
    JCI Insight, 2020-08-20;5(16):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  26. Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
    Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
    Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  27. Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice
    Authors: Y Takagaki, S Shi, M Katoh, M Kitada, K Kanasaki, D Koya
    Sci Rep, 2019-05-17;9(1):7519.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Hepatocyte-secreted DPP4 in obesity promotes adipose inflammation and insulin resistance
    Authors: DS Ghorpade, L Ozcan, Z Zheng, SM Nicoloro, Y Shen, E Chen, M Blüher, MP Czech, I Tabas
    Nature, 2018-03-21;555(7698):673-677.
    Species: Mouse
    Sample Types: Plasma
    Applications: Immunodepletion
  29. Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis
    Sci Rep, 2016-07-18;6(0):29884.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury.
    Authors: Chen Y, Chen H, Chien C, Wu S, Ho Y, Yu C, Chang M
    PLoS ONE, 2015-08-26;10(8):e0134327.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  31. Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
    Authors: Kanasaki K, Shi S, Kanasaki M, He J, Nagai T, Nakamura Y, Ishigaki Y, Kitada M, Srivastava S, Koya D
    Diabetes, 2014-02-26;63(6):2120-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  33. KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.
    Authors: Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton D, Bissig K, Kalir T, Jacobs A, Evans T, Evans M, Gouon-Evans V
    Cell Stem Cell, 2013-06-06;12(6):748-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. Inhibition of dipeptidyl peptidase 4 regulates microvascular endothelial growth induced by inflammatory cytokines.
    Authors: Takasawa W, Ohnuma K, Hatano R, Endo Y, Dang NH, Morimoto C
    Biochem. Biophys. Res. Commun., 2010-09-07;401(1):7-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  35. Epidermal beta -catenin activation remodels the dermis via paracrine signalling to distinct fibroblast lineages
    Authors: Beate M. Lichtenberger, Maria Mastrogiannaki, Fiona M. Watt
    Nature Communications
  36. Tissue Repair Signals and In Vitro Culture: Inflammatory Cytokine TNF alpha Promotes the Expansion of Primary Hepatocytes in 3D Culture
    Authors: Weng Chuan Peng, Catriona Y. Logan, Matt Fish, Teni Anbarchian, Francis Aguisanda, Adrián Álvarez-Varela et al.
    Cell
  37. Brush border protocadherin CDHR2 promotes the elongation and maximized packing of microvilli in vivo
    Authors: Pinette JA, Mao S, Millis BA et al.
    Mol. Biol. Cell

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Mouse DPPIV/CD26 Antibody
By Anonymous on 10/18/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: mesenchymal cells Species: Human
Immunocytochemistry/Immunofluorescence Mouse DPPIV/CD26 Antibody AF954
Mouse DPPIV/CD26 Antibody
By Anonymous on 08/11/2016
Application: IP Sample Tested: Heparin Plasma Species: Mouse