Mouse EphA8 Antibody

Catalog #: AF454 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF454
AF454-SP

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Mouse EphA8 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse EphA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant rat EphA5, recombinant mouse (rm) EphA6, rmEphA7, rmEphA2, recombinant human EphA1, and rmEphA3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EphA8
Gly27 - Arg540
Accession # O09127

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse EphA8 Fc Chimera (Catalog # 454-A8)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA8

EphA8, also known as Eek and Hek3 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA8 has been shown to bind ephrin‑A5, ephrin‑A3, and ephrin‑A2 (2, 3). The full-length human and rat homologs have not been cloned. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

Eph Nomenclature Committee [letter] (1997) Cell 90:403.
Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Long Name

Eph Receptor A8

Entrez Gene IDs

2046 (Human); 13842 (Mouse)

Alternate Names

Eek; EK3; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; EPH receptor A8; EphA8; EPH-like kinase 3; ephrin type-A receptor 8; Hek3; hydroxyaryl-protein kinase; KIAA1459; protein-tyrosine kinase; tyrosine-protein kinase receptor EEK; tyrosylprotein kinase