Mouse FoxN1 Antibody Summary
Val2-Asp164
Accession # Q61575
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
FoxN1 in Mouse Liver. FoxN1 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse FoxN1 Monoclonal Antibody (Catalog # MAB7295) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxN1
Forkhead box N1 (FoxN1) is a 69 kDa transcriptional regulator that is primarily expressed in thymic epithelial cells and keratinocytes. FoxN1 function is required for proper T cell, hair follicle, and nail differentiation. Mutations in FoxN1 result in severe combined immunodeficiency (SCID/Nude). Mouse FoxN1 contains one DNA-binding forkhead domain (aa 271-367). Within amino acids 1 - 164, mouse FoxN1 shares 84% and 95% aa sequence identity with human and rat FoxN1, respectively.
Product Datasheets
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