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Mouse Frizzled-7 Antibody

Catalog #: AF198
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF198
AF198-SP
Detection of Mouse Frizzled‑7 by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Frizzled-7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) Frizzled-2 and less than 5% cross-reactivity with rmFrizzled-3, rmFrizzled-4, rhFrizzled-5, rmFrizzled-6, rmFrizzled-8, and rmFrizzled-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185
Accession # Q61090
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Frizzled-7 antibody by Western Blot. View Larger

Detection of Mouse Frizzled‑7 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Frizzled-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF198) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Frizzled-7 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Frizzled-7 antibody in Embryonic Mouse Gastrointestinal Tract by Immunohistochemistry (IHC-Fr). View Larger

Frizzled‑7 in Embryonic Mouse Gastrointestinal Tract. Frizzled-7 was detected in immersion fixed frozen sections of embryonic mouse gastrointestinal tract (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF198) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-7

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises the Wnt binding site, spans about 130 amino acid residues and contains ten invariant cysteine residues. Mouse Frizzled-7 contains 572 amino acid residues and shares 99% identity with the human orthologue in the CRD (3, 4). Frizzled-7 mRNA has been detected in relatively large amounts in adult skeletal muscle, plancenta and heart, and fetal kidney and lung (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Frizzled-7 can activate canonical Wnt/ beta -Catenin signaling, as well as a non-canonical pathway involved in tissue morphogenesis (4, 5).

References
  1. Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
  2. Hsieh, J-C. (2004) Front. Biosci. 9:1333.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Sheldahl, L.C. et al. (1999) Curr. Biol. 9:695.
  5. Medina, A. et al. (2000) Mech. Dev. 92:227.
Entrez Gene IDs
8324 (Human); 14369 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7

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Citations for Mouse Frizzled-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Cftr Modulates Wnt/?-Catenin Signaling and Stem Cell Proliferation in Murine Intestine
    Authors: AM Strubberg, J Liu, NM Walker, CD Stefanski, RJ MacLeod, ST Magness, LL Clarke
    Cell Mol Gastroenterol Hepatol, 2017-12-07;5(3):253-271.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016-07-11;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Frizzled 7 and PIP2 binding by syntenin PDZ2 domain supports Frizzled 7 trafficking and signalling
    Nat Commun, 2016-07-08;7(0):12101.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  4. The postsynaptic density 95/disc-large/zona occludens protein syntenin directly interacts with frizzled 7 and supports noncanonical Wnt signaling.
    Authors: Luyten A, Mortier E, Van Campenhout C, Taelman V, Degeest G, Wuytens G, Lambaerts K, David G, Bellefroid EJ, Zimmermann P
    Mol. Biol. Cell, 2008-02-06;19(4):1594-604.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Regulation of endothelial cell cytoskeletal reorganization by a secreted frizzled-related protein-1 and frizzled 4- and frizzled 7-dependent pathway: role in neovessel formation.
    Authors: Dufourcq P, Leroux L, Ezan J, Descamps B, Lamaziere JM, Costet P, Basoni C, Moreau C, Deutsch U, Couffinhal T, Duplaa C
    Am. J. Pathol., 2007-12-21;172(1):37-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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