Mouse GDF-5/BMP-14 Antibody Summary
Ala376-Arg495
Accession # P43027
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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GDF‑5 in Mouse Embryo. GDF-5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Mouse GDF‑5/BMP‑14 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse GDF‑5/BMP‑14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF‑5/BMP‑14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-5/BMP-14
Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region, and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6, and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).
- Storm, E.E. et al. (1994) Nature 368:639.
- Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
- Francis-West, P.H. et al. (1999) Development 126:1035.
- Massague, J. et al. (2000) Genes and Dev. 14:627.
- Settle, S.H. Jr. et al. (2003) Dev. Biol. 254:116.
- Inada, M. et al. (1996) BBRC 222:317.
Product Datasheets
Citations for Mouse GDF-5/BMP-14 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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HOXA10 promotes Gdf5 expression in articular chondrocytes
Authors: Murakami, T;Ruengsinpinya, L;Takahata, Y;Nakaminami, Y;Hata, K;Nishimura, R;
Scientific reports
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
GDF5 Mediated Enhancement of Chondrocyte Phenotype is Inhibited by Heparin; Implication for the use of Heparin in the Clinic and in Tissue Engineering Applications
Authors: Bethanie Imogen Ayerst
Tissue Eng Part A, 2017-01-06;0(0):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors.
Authors: Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A
Stem Cells Dev, 2013-02-15;22(9):1342-59.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Structural and functional analysis of intra-articular interzone tissue in axolotl salamanders
Authors: Rebekah S. Cosden-Decker, Melissa M. Bickett, Christian Lattermann, James N. MacLeod
Osteoarthritis and Cartilage
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Growth differentiation factor 5 regulates cardiac repair after myocardial infarction.
Authors: Zaidi SH, Huang Q, Momen A, Riazi A, Husain M
J. Am. Coll. Cardiol., 2010-01-12;55(2):135-43.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Freeze-dried tendon allografts as tissue-engineering scaffolds for Gdf5 gene delivery.
Authors: Basile P, Dadali T, Jacobson J, Hasslund S, Ulrich-Vinther M, Soballe K, Nishio Y, Drissi MH, Langstein HN, Mitten DJ, O'Keefe RJ, Schwarz EM, Awad HA
Mol. Ther., 2008-01-08;16(3):466-73.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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