Mouse Hip Antibody

Catalog #: MAB1568 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1568
MAB1568-SP

All Hip Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Hip Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Hip in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 217413

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Hip
Met1-Arg678
Accession # AAD31172

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Hip (Catalog # 1568-HP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hip

Hedgehog signaling proteins act as mitogens, morphogens, or inducing factors in many different cell types during embryonic development. They aid in growth, patterning, and morphogenesis in both vertebrates and insects (1). Hip (Hedgehog-interacting protein) is a type I transmembrane protein identified for its ability to bind biologically active Sonic Hedgehog. It is comprised of 700 aa, and includes a hydrophobic signal sequence, two EGF-like domains near the C-terminus, and a 22 amino acid membrane-spanning region at the C-terminal end (2). Hip has only been identified in vertebrates and binds all three mammalian Hedgehogs: sonic (Shh), desert (Dhh), and Indian (Ihh). Like the Hedgehog receptor Patched, Hip is a transcriptional target of Hedgehog signaling (2). Unlike Patched, Hip’s ability to bind hedgehogs is not involved in transducing a signal intracellularly, rather it regulates the availability of Hedgehog ligand extracellularly (3). Transgenic mice overexpressing Hip in proliferating chondrocytes display skeletal defects similar to those observed in Ihh mutant mice. These results indicate that Hip is involved in attenuating Hedgehog signaling (2). The expression pattern of Hip correlates with its ability to interact with all three mammalian Hedgehogs. It is expressed in a variety of organs, adjacent with sites of hedgehog expression. For instance, Shh is expressed in the epithelium of the lung, and Hip is found in the underlying lung mesenchyme (2). In fact, Hip knock-out mice exhibit neonatal lethality with respiratory failure due to defective branching morphogenesis. This phenotype correlates with altered expression of Shh markers suggesting an increase in Shh signaling (3). Interestingly, other developmental mechanisms that rely on normal Shh signaling, such as dorsal-ventral patterning of the neural tube, development of the somites, and organ laterality appeared histologically normal in Hip^-/- mice (3). Mouse and human Hip share 94% amino acid identity through the entire protein sequence (4).

References