Mouse IFN-beta Antibody

Catalog #: MAB82342 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB82342-100
MAB82342-SP

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All IFN-beta Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-beta Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN‑ beta in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 944233

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived Mouse IFN‑ beta
Ile22-Asn182
Accession # P01575

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse IFN‑ beta Monoclonal Antibody (Catalog # MAB82341).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IFN-beta DuoSet ELISA Kit (Catalog # DY8234-05) for convenient development of a sandwich ELISA or the Mouse IFN-beta Quantikine ELISA Kit (Catalog # MIFNB0) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Mouse IFN‑beta ELISA Standard Curve. Recombinant Mouse IFN‑beta protein was serially diluted 2-fold and captured by Rat Anti-Mouse IFN‑beta Monoclonal Antibody (Catalog # MAB82342) coated on a Clear Polystyrene Microplate (DY990). Rat Anti-Mouse IFN‑beta Monoclonal Antibody (MAB82341) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

References

González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
Häcker, H. et al. (2006) Nature 439:204.
Teige, I. et al. (2003) J. Immunol. 170:4776.
Shinohara, M.L. et al. (2008) Immunity 29:68.
Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
Wang, H. et al. (2011) J. Immunol 186:675.