Mouse IL-10 Antibody

Catalog #: MAB4172 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4172-100
MAB4172-SP

All IL-10 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-10 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-10 in ELISA.

Source

Monoclonal Rat IgG_2A Clone # 880429

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived mouse IL-10 protein
Ser19-Ser178
Accession # NP_034678

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with rat anti-mouse IL-10 monoclonal antibody (Catalog # MAB417).This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References

Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
Hsu, D.-H. et al. (1990) Science 250:830.
Moore, K. et al. (1990) Science 248:1230.
Windsor, W.T. et al. (1993) Biochemistry 32:8807.
Syto, R. et al. (1998) Biochemistry 37:16943.
Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF