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Mouse IL-36 alpha /IL-1F6 Antibody

Catalog #: AF2297
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2297
AF2297-SP
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-36 alpha /IL-1F6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL-1F5, rhIL-1F6, and rhIL-1F9 is observed, and less than 2% cross-reactivity with recombinant mouse IL‑1F8, rhIL-1F7, rhIL-1 alpha, rhIL-1 beta, rhIL-1ra, and rhIL-18 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑36 alpha /IL‑1F6
Met1-His160
Accession # Q9JLA2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑36 alpha /IL‑1F6 (Catalog # 2297-ML)
ELISA
This antibody functions as an ELISA capture antibody when paired with mouse IL‑36 alpha /IL‑1F6 Antibody (Catalog # MAB22972). This product is intended for assay development on various assay platforms requiring antibody pairs.
 
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.6-2.4 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Mouse IL-1 Rrp2/IL-1 R6 Fc Chimera (Catalog # 2354-RP) to immobilized Recombinant Mouse IL‑36 alpha /IL‑1F6 (Catalog # 2297-ML) coated at 1 µg/mL (100 µL/well). At 40 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Mouse interleukin-36 alpha [IL-36 alpha ; also named interleukin 1 family member 6, IL-1F6 and FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL-1 beta, IL‑1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F6 is synthesized as a 160 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5). It appears to be actively secreted (1). When found in cell lysates, it presents as an 18 kDa monomer (2). Mouse to human, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 29% aa identical to IL-1ra, and 32%, 32%, 34%, 41%, and 28% aa identical to IL-1 beta, IL-1F5, F8, F9, and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells (1, 4). Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL‑1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL‑1F6 may signal in similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Debets, R. et al. (2001) J. Immunol. 167:1440.
  6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Entrez Gene IDs
27179 (Human); 54448 (Mouse)
Alternate Names
FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha

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Citations for Mouse IL-36 alpha /IL-1F6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Unprocessed Interleukin-36alpha Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1.
    Authors: Milora K, Fu H, Dubaz O, Jensen L
    J Invest Dermatol, 2015-07-23;135(12):2992-3000.
  2. MES SV40 Cells Are Sensitive to Lipopolysaccharide, Peptidoglycan, and Poly I:C Expressing IL-36 Cytokines
    Authors: CG Pelcastre-, EA Vazquez-Sa, JM Murrieta-C, S Rodríguez-, JC Cancino-Di, ME Cancino-Di
    International Journal of Molecular Sciences, 2022-10-07;23(19):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  3. Epithelial cells release IL-36 alpha in extracellular vesicles following mechanical damage
    Authors: Drake Winslow Williams, Reuben H. Kim
    Biochemical and Biophysical Research Communications
  4. Targeting IL-36 improves age-related coronary microcirculatory dysfunction and attenuates myocardial ischaemia-reperfusion injury in mice
    Authors: J El-Awaisi, DP Kavanagh, MR Rink, CJ Weston, NE Drury, N Kalia
    JCI Insight, 2022-03-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. The Protective Role of IL-36/IL-36R Signal in Con A–Induced Acute Hepatitis
    Authors: Xiaofang Wang, Yuejin Liang, Hui Wang, Biao Zhang, Lynn Soong, Jiyang Cai et al.
    The Journal of Immunology
  6. Analysis of Il36a induction by C/EBPbeta via a half-CRE�C/EBP element in murine macrophages in dependence of its CpG methylation level
    Authors: A Nerlich, N Janze, R Goethe
    Genes and immunity, 2021-10-25;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Disrupting the IL-36 and IL-23/IL-17 loop underlies the efficacy of calcipotriol and corticosteroid therapy for psoriasis
    Authors: B Germán, R Wei, P Hener, C Martins, T Ye, C Gottwick, J Yang, J Seneschal, K Boniface, M Li
    JCI Insight, 2019-01-24;4(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Staphylococcus aureus Virulent PSM alpha Peptides Induce Keratinocyte Alarmin Release to Orchestrate IL-17-Dependent Skin Inflammation
    Authors: Seitaro Nakagawa, Masanori Matsumoto, Yuki Katayama, Rena Oguma, Seiichiro Wakabayashi, Tyler Nygaard et al.
    Cell Host & Microbe
  9. Interleukin-36 receptor mediates the crosstalk between plasma cells and synovial fibroblasts
    Authors: V Schmitt, M Hahn, V Kästele, O Wagner, M Wiendl, A Derer, A Taddeo, S Hahne, A Radbruch, HM Jäck, W Schuh, D Mielenz, S Gay, G Schett, AJ Hueber, S Frey
    Eur. J. Immunol., 2017-09-22;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Local CD34-positive capillaries decrease in mouse models of kidney disease associating with the severity of glomerular and tubulointerstitial lesions
    Authors: MA Masum, O Ichii, YHA Elewa, T Nakamura, Y Kon
    BMC Nephrol, 2017-09-04;18(1):280.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011-01-17;186(4):2613-22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Externalization of the leaderless cytokine IL-1F6 occurs in response to lipopolysaccharide/ATP activation of transduced bone marrow macrophages.
    Authors: Martin U, Scholler J, Gurgel J, Renshaw B, Sims JE, Gabel CA
    J. Immunol., 2009-08-28;183(6):4021-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. IL-36 Induces Bisphosphonate-Related Osteonecrosis of the Jaw-Like Lesions in Mice by Inhibiting TGF-?-Mediated Collagen Expression
    J Bone Miner Res, 2016-10-12;0(0):.

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Mouse IL‑36 alpha /IL‑1F6 Antibody
By Anonymous on 11/16/2020
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse