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Mouse IRF1 Antibody

Catalog #: AF4715
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4715
AF4715-SP
Detection of Mouse IRF1 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IRF1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IRF1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IRF1
Thr147-Pro329
Accession # P15314
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IRF1 antibody by Western Blot. View Larger

Detection of Mouse IRF1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse IRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4715) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for IRF1 was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF1

IRF1 is a member of the interferon regulator transcription factor family, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF1 knock out mice show defects in NK and T cell function, as well as, develop CD30+ lymphoproliferative disease.

Long Name
Interferon Regulatory Factor 1
Entrez Gene IDs
3659 (Human); 16362 (Mouse); 24508 (Rat)
Alternate Names
interferon regulatory factor 1; IRF1; MAR; MARIRF-1

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Citations for Mouse IRF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Bacterial recognition by TLR7 in the lysosomes of conventional dendritic cells.
    Authors: Mancuso G, Gambuzza M, Midiri A, Biondo C, Papasergi S, Akira S, Teti G, Beninati C
    Nat. Immunol., 2009-06-01;10(6):587-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Gene Regulatory Network Inference of Immunoresponsive Gene 1 (IRG1) Identifies Interferon Regulatory Factor 1 (IRF1) as Its Transcriptional Regulator in Mammalian Macrophages
    Authors: Aravind Tallam, Thaneer M. Perumal, Paul M. Antony, Christian Jäger, Joëlle V. Fritz, Laurent Vallar et al.
    PLOS ONE

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