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Mouse Klotho  beta Antibody

Catalog #: AF2619
16 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2619
AF2619-SP
Product Details
Citations (16)
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Mouse Klotho  beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Klotho beta  in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant mouse Klotho is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Klotho beta (R&D Systems, Catalog # 2619-KB)
Phe53-Leu995
Accession # NP_112457
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Klotho  beta (Catalog # 2619-KB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klotho beta

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF15 (human FGF19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2 - 4). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2 - 3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.

References
  1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
  2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
  3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
  5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
  6. Chang, Q. et al. (2005) Science 310:490.
  7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
Entrez Gene IDs
152831 (Human); 83379 (Mouse); 289625 (Rat); 102147155 (Cynomolgus Monkey)
Alternate Names
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; klotho beta-like protein; MGC142213

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Citations for Mouse Klotho  beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. MiR-34a inhibits beige and brown fat formation in obesity in part by suppressing adipocyte FGF21 signaling and SIRT1 function.
    Authors: Fu T, Seok S, Choi S et al.
    Mol Cell Biol.
  2. Fibroblast growth factor 21 Ameliorates diabetes-induced endothelial dysfunction in mouse aorta via activation of the CaMKK2/AMPK alpha signaling pathway
    Authors: Lei Ying, Na Li, Zhengyue He, Xueqin Zeng, Yan Nan, Jiantong Chen et al.
    Cell Death & Disease
  3. Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation
    Authors: Shirong Wang, Lin Zha, Xin Cui, Yu‐Te Yeh, Ruochuan Liu, Jia Jing et al.
    Advanced Science
  4. Sustained Brown Fat Stimulation and Insulin Sensitization by a Humanized Bispecific Antibody Agonist for Fibroblast Growth Factor Receptor 1/ beta Klotho Complex
    Authors: Ganesh Kolumam, Mark Z. Chen, Raymond Tong, Jose Zavala-Solorio, Lance Kates, Nicholas van Bruggen et al.
    EBioMedicine
  5. FGF21 resistance is not mediated by downregulation of beta-klotho expression in white adipose tissue
    Authors: Kathleen R. Markan, Meghan C. Naber, Sarah M. Small, Lila Peltekian, Rachel L. Kessler, Matthew J. Potthoff
    Molecular Metabolism
  6. Sulforaphane ameliorates non-alcoholic fatty liver disease in mice by promoting FGF21/FGFR1 signaling pathway
    Authors: YK Wu, ZN Ren, SL Zhu, YZ Wu, G Wang, H Zhang, W Chen, Z He, XL Ye, QX Zhai
    Acta pharmacologica Sinica, 2021-10-15;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action
    Authors: CA Gilroy, S Roberts, A Chilkoti
    J Control Release, 2018-03-15;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. FGF21 Regulates Metabolism Through Adipose-Dependent and -Independent Mechanisms
    Authors: LD BonDurant, M Ameka, MC Naber, KR Markan, SO Idiga, MR Acevedo, SA Walsh, DM Ornitz, MJ Potthoff
    Cell Metab., 2017-04-04;25(4):935-944.e4.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Skeletal muscle mitochondrial uncoupling drives endocrine cross-talk through the induction of FGF21 as a myokine.
    Authors: Keipert S, Ost M, Johann K, Imber F, Jastroch M, van Schothorst E, Keijer J, Klaus S
    Am J Physiol Endocrinol Metab, 2013-12-17;306(5):E469-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Aberrantly elevated microRNA-34a in obesity attenuates hepatic responses to FGF19 by targeting a membrane coreceptor beta-Klotho.
    Authors: Fu T, Choi S, Kim D, Seok S, Suino-Powell K, Xu H, Kemper J
    Proc Natl Acad Sci U S A, 2012-09-17;109(40):16137-42.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  11. FGF21 Promotes Metabolic Homeostasis via White Adipose and Leptin in Mice.
    Authors: Veniant MM, Hale C, Helmering J, Chen MM, Stanislaus S, Busby J, Vonderfecht S, Xu J, Lloyd DJ
    PLoS ONE, 2012-07-06;7(7):e40164.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Fibroblast Growth Factor 21 (FGF21) Inhibits Chondrocyte Function and Growth Hormone Action Directly at the Growth Plate.
    Authors: Wu S, Levenson A, Kharitonenkov A, De Luca F
    J. Biol. Chem., 2012-06-13;287(31):26060-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Metalloproteinase-mediated Shedding of Integrin beta2 promotes macrophage efflux from inflammatory sites.
    Authors: Gomez IG, Tang J, Wilson CL, Yan W, Heinecke JW, Harlan JM, Raines EW
    J. Biol. Chem., 2011-12-14;287(7):4581-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  14. Different roles of N- and C- termini in the functional activity of FGF21.
    Authors: Micanovic R, Raches DW, Dunbar JD, Driver DA, Bina HA, Dickinson CD, Kharitonenkov A
    J. Cell. Physiol., 2009-05-01;219(2):227-34.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. BetaKlotho is required for metabolic activity of fibroblast growth factor 21.
    Authors: Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M
    Proc. Natl. Acad. Sci. U.S.A., 2007-04-23;104(18):7432-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Feto-maternal cholesterol transport regulated by beta -Klotho–FGF15 axis is essential for fetal growth
    Authors: Kanako Kobayashi, Kazuko Iwasa, Rika Azuma-Suzuki, Takeshi Kawauchi, Yo-ichi Nabeshima
    Life Science Alliance

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