Mouse PDGF-C Antibody

PDGF‑C in Mouse Kidney. PDGF‑C was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1447) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Cell Proliferation Induced by PDGF‑CC and Neutralization by Mouse PDGF‑C Antibody. Recombinant Mouse PDGF‑CC stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.8 µg/mL Recombinant Mouse PDGF‑CC is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF-C Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF1447). The ND₅₀ is typically 4‑16 µg/mL.

Detection of Mouse PDGF-C by Immunocytochemistry/ Immunofluorescence FREM1 regulates activation of AKT and MAPK upon PDGFC stimulation. (A) Representative western blotting of phosphorylation of AKT and MAPK ERK1/2 in MEFs from WT and bat mouse embryos stimulated with PDGFCC for the indicated time periods. (B) Relative quantification of AKT phosphorylation levels. WT cells 10 minutes after stimulation were assigned a value of 1 and all other samples are standardised against this value. Graph represents average of up to nine WT and 16 bat samples, performed across four independent experiments from at least three different cell lines for each genotype. Black bars: WT; white bars, bat mutant. (C) FREM1 mutation in bat mutants reduces phosphorylation of PDGFR alpha in response to the addition of exogenous PDGFCC. IP, immunoprecipitation antibody; WB, western blotting antibody. (D) E13.5 WT embryo head skin sections stained for PDGFC (green), PDGFR alpha (red) and nuclear dye DAPI (blue). Error bars represent standard error of the mean (s.e.m.); *P<0.05, **P<0.01, ***P<0.005. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24046351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse PDGF-C by Immunocytochemistry/ Immunofluorescence FREM1 regulates activation of AKT and MAPK upon PDGFC stimulation. (A) Representative western blotting of phosphorylation of AKT and MAPK ERK1/2 in MEFs from WT and bat mouse embryos stimulated with PDGFCC for the indicated time periods. (B) Relative quantification of AKT phosphorylation levels. WT cells 10 minutes after stimulation were assigned a value of 1 and all other samples are standardised against this value. Graph represents average of up to nine WT and 16 bat samples, performed across four independent experiments from at least three different cell lines for each genotype. Black bars: WT; white bars, bat mutant. (C) FREM1 mutation in bat mutants reduces phosphorylation of PDGFR alpha in response to the addition of exogenous PDGFCC. IP, immunoprecipitation antibody; WB, western blotting antibody. (D) E13.5 WT embryo head skin sections stained for PDGFC (green), PDGFR alpha (red) and nuclear dye DAPI (blue). Error bars represent standard error of the mean (s.e.m.); *P<0.05, **P<0.01, ***P<0.005. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24046351), licensed under a CC-BY license. Not internally tested by R&D Systems.