Mouse/Rat Integrin  alpha 8 Antibody

Detection of Mouse Integrin alpha 8 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Integrin alpha 8 in 4T1 Mouse Breast Cancer Cell Line. Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse and Rat Integrin alpha 8 by Simple Western^TM. Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.