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Mouse TSLP Antibody

Catalog #: AF555
35 Citations Datasheet / COA / SDS
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AF555
AF555-SP
Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.
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Citations (35)
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Mouse TSLP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TSLP in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TSLP (Catalog # 555-TS)
Neutralization
Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 [Park, L.S. et al. (2000) J. Exp. Med. 192:659]. The Neutralization Dose (ND50) is typically ≤0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. View Larger

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. Recombinant Mouse TSLP (555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND50 is typically ≤0.4 µg/mL.

Immunohistochemistry-Paraffin Detection of Mouse TSLP by Immunohistochemistry-Paraffin View Larger

Detection of Mouse TSLP by Immunohistochemistry-Paraffin Klk5 deletion results in remarkable suppression of the inflammatory phenotype of NS.(A-E). Results show remarkable abrogation of pro-allergic and pro-inflammatory cytokines quantified by RT-qPCR. (A) Il-1 beta, Tslp, Tnf-alpha ; (B) Il-6, Il-18, the type 17 promoting cytokine Il-23p19; (C) Ifn-gamma, Il-4, Il-17A, Il-22; (D) Th17 regulated genes Defb4, Slpi, Cxcl1 and Ccl20; (E) Th17/22 regulated genes s100a7, s100a8, s100a9. Data are shown as the mean ± s.e.m. of triplicate amplification for at least five mice per genotype. Results are normalized to wt mean (set as 1.0). *p<0.05, **p<0.005, ***p<0.001 (Mann-Whitney U test). (F) Quantification of mast cells based on toluidine blue staining of skin tissue (mast cells stain purple). An increased number of mast cells was detected in Spink5-/- dermis; (G) IHC analysis shows abolishment of Tslp expression in Spink5-/-Klk5-/- skin as compared to Spink5-/-; Scale bar: 50μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26390218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry-Paraffin Detection of Mouse TSLP by Immunohistochemistry-Paraffin View Larger

Detection of Mouse TSLP by Immunohistochemistry-Paraffin Klk5 deletion results in remarkable suppression of the inflammatory phenotype of NS.(A-E). Results show remarkable abrogation of pro-allergic and pro-inflammatory cytokines quantified by RT-qPCR. (A) Il-1 beta, Tslp, Tnf-alpha ; (B) Il-6, Il-18, the type 17 promoting cytokine Il-23p19; (C) Ifn-gamma, Il-4, Il-17A, Il-22; (D) Th17 regulated genes Defb4, Slpi, Cxcl1 and Ccl20; (E) Th17/22 regulated genes s100a7, s100a8, s100a9. Data are shown as the mean ± s.e.m. of triplicate amplification for at least five mice per genotype. Results are normalized to wt mean (set as 1.0). *p<0.05, **p<0.005, ***p<0.001 (Mann-Whitney U test). (F) Quantification of mast cells based on toluidine blue staining of skin tissue (mast cells stain purple). An increased number of mast cells was detected in Spink5-/- dermis; (G) IHC analysis shows abolishment of Tslp expression in Spink5-/-Klk5-/- skin as compared to Spink5-/-; Scale bar: 50μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26390218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLP

Stromal Lymphopoietin (TSLP) was originally identified from the conditioned medium of a mouse thymic stromal cell line as a protein that promoted the development of B cells. The activity of mouse TSLP overlaps with, but is distinct from, that of mouse IL-7 (1). Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites. The Sf 21 cell expressed rmTSLP is likely to be glycosylated at all three sites, as three major glycoforms were visible on SDS-PAGE (Figure 1). Insect cells are known to express relatively simple and homogeneous N-glycans that mainly belong to the high mannose type (2). This recombinant protein was found to be an excellent substrate for N-specific glycosidases such as Endo F3 (Figure 1). The majority of the glycans on rmTSLP can be readily removed by Endo F3. However, a small percentage of the glycans is somewhat resistant to Endo F3 digestion, possibly lacking core fucose, as it is known that core fucosylated N-glycans are strongly preferred substrates for Endo F3 digestion (3).

References
  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Staudacher, E. et al. (1992) Eur. J. Biochem. 207:987.
  3. Tarentino, A.L. and T.H. Jr. Plummer (1994) Glycobiology 4: 771.
Long Name
Thymic Stromal Lymphopoietin
Entrez Gene IDs
85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)
Alternate Names
thymic stromal lymphopoietin; TSLP

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Citations for Mouse TSLP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Identification of FUBP1 as a Long Tail Cancer Driver and Widespread Regulator of Tumor Suppressor and Oncogene Alternative Splicing
    Authors: Elman JS, Ni TK, Mengwasser KE et al.
    Cell Rep
  2. Tissue-Specific Distribution of iNKT Cells Impacts Their Cytokine Response.
    Authors: Lee YJ Wang H Starrett GJ et al.
    Immunity.
  3. Soluble MAC is primarily released from MAC-resistant bacteria that potently convert complement component C5
    Authors: DJ Doorduijn, MV Lukassen, MFL van 't Wou, V Franc, M Ruyken, BW Bardoel, AJR Heck, SHM Rooijakker
    Elife, 2022-08-10;11(0):.
  4. Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
    Authors: H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd
    Mucosal Immunology, 2022-07-15;0(0):.
  5. Drug-triggered and cross-linked self-assembling nanofibrous hydrogels
    Authors: Kumar VA, Shi S, Wang BK et al.
    J. Am. Chem. Soc.
  6. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature
    Authors: Ingelfinger F, Krishnarajah S, Kramer M et al.
    Acta neuropathologica
  7. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair
    Authors: JA Lindborg, NM Tran, DM Chenette, K DeLuca, Y Foli, R Kannan, Y Sekine, X Wang, M Wollan, IJ Kim, JR Sanes, SM Strittmatt
    Cell Reports, 2021-03-02;34(9):108777.
  8. Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia
    Authors: Diana Cadena Castaneda, Sonia Jangra, Marina Yurieva, Jan Martinek, Megan Callender, Matthew Coxe et al.
    iScience
  9. Unlocking the Role of Endothelial MPL Receptor and JAK2V617F Mutation: Insights into Cardiovascular Dysfunction in MPNs and CHIP
    Authors: Zhang, H;Kafeiti, N;Lee, S;Masarik, K;Zheng, H;Zhan, H;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  10. Signalling by senescent melanocytes hyperactivates hair growth
    Authors: Wang, X;Ramos, R;Phan, AQ;Yamaga, K;Flesher, JL;Jiang, S;Oh, JW;Jin, S;Jahid, S;Kuan, CH;Nguyen, TK;Liang, HY;Shettigar, NU;Hou, R;Tran, KH;Nguyen, A;Vu, KN;Phung, JL;Ingal, JP;Levitt, KM;Cao, X;Liu, Y;Deng, Z;Taguchi, N;Scarfone, VM;Wang, G;Paolilli, KN;Wang, X;Guerrero-Juarez, CF;Davis, RT;Greenberg, EN;Ruiz-Vega, R;Vasudeva, P;Murad, R;Widyastuti, LHP;Lee, HL;McElwee, KJ;Gadeau, AP;Lawson, DA;Andersen, B;Mortazavi, A;Yu, Z;Nie, Q;Kunisada, T;Karin, M;Tuckermann, J;Esko, JD;Ganesan, AK;Li, J;Plikus, MV;
    Nature
  11. Non-Expressed Donor KIR3DL1 Alleles May Represent a Risk Factor for Relapse after T-Replete Haploidentical Hematopoietic Stem Cell Transplantation
    Authors: Legrand, N;Salameh, P;Jullien, M;Chevallier, P;Ferron, E;David, G;Devilder, MC;Willem, C;Gendzekhadze, K;Parham, P;Retière, C;Gagne, K;
    Cancers
  12. Human CEACAM1 is targeted by a Streptococcus pyogenes adhesin implicated in puerperal sepsis pathogenesis
    Authors: EA Catton, DA Bonsor, C Herrera, M Stålhammar, M Lyndin, CE Turner, J Soden, JAG van Strijp, BB Singer, NM van Sorge, G Lindahl, AJ McCarthy
    Nature Communications, 2023-04-20;14(1):2275.
  13. Macrophages promote anti-androgen resistance in prostate cancer bone disease
    Authors: Li XF, Selli C, Zhou HL et al.
    The Journal of experimental medicine
  14. Multivalent in vivo delivery of DNA-encoded bispecific T cell engagers effectively controls heterogeneous GBM tumors and mitigates immune escape
    Authors: Park D, Liaw K, Bhojnagarwala P et al.
    Molecular Therapy - Oncolytics
  15. Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice
    Authors: Zhao W, Chen L, Tan W et al.
    Foods
  16. Repair of Limb Ischemia Is Dependent on Hematopoietic Stem Cell Specific-SHP-1 Regulation of TGF-beta 1
    Authors: Chen Wang, Ravi Nistala, Min Cao, De-Pei Li, Yi Pan, Mojgan Golzy et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  17. Integrated Analytical System for Clinical Single‐Cell Analysis
    Authors: Hannah M. Peterson, Lip Ket Chin, Yoshi Iwamoto, Juhyun Oh, Jonathan C. T. Carlson, Hakho Lee et al.
    Advanced Science
  18. Angiogenin Released from ABCB5+ Stromal Precursors Improves Healing of Diabetic Wounds by Promoting Angiogenesis
    Authors: Karmveer Singh, Pallab Maity, Albert Kallon Koroma, Abhijit Basu, Rajeev Kumar Pandey, Seppe Vander Vander Beken et al.
    Journal of Investigative Dermatology
  19. Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification
    Authors: Grace A. Christopher, Rebecca J. Noort, Jessica L. Esseltine
    Biomolecules
  20. Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division
    Authors: Sergi Junyent, Joshua C Reeves, James LA Szczerkowski, Clare L Garcin, Tung-Jui Trieu, Matthew Wilson et al.
    eLife
  21. IL-33 promotes anemia during chronic inflammation by inhibiting differentiation of erythroid progenitors
    Authors: James W. Swann, Lada A. Koneva, Daniel Regan-Komito, Stephen N. Sansom, Fiona Powrie, Thibault Griseri
    Journal of Experimental Medicine
  22. Skeletal muscle fibrosis is associated with decreased muscle inflammation and weakness in patients with chronic kidney disease
    Authors: Matthew K. Abramowitz, William Paredes, Kehao Zhang, Camille R. Brightwell, Julia N. Newsom, Hyok-Joon Kwon et al.
    American Journal of Physiology-Renal Physiology
  23. Injury-induced perivascular niche supports alternative differentiation of adult rodent CNS progenitor cells
    Authors: Justyna Ulanska-Poutanen, Jakub Mieczkowski, Chao Zhao, Katarzyna Konarzewska, Beata Kaza, Hartmut BF Pohl et al.
    eLife
  24. Feasibility study of the Fab fragment of a monoclonal antibody against tissue factor as a diagnostic tool
    Authors: RYO TSUMURA, RYUTA SATO, FUMIAKI FURUYA, YOSHIKATSU KOGA, YOSHIYUKI YAMAMOTO, YUKI FUJIWARA et al.
    International Journal of Oncology
  25. KLK5 Inactivation Reverses Cutaneous Hallmarks of Netherton Syndrome
    Authors: Laetitia Furio, Georgios Pampalakis, Iacovos P. Michael, Andras Nagy, Georgia Sotiropoulou, Alain Hovnanian
    PLOS Genetics
  26. Self-assembling multidomain peptides tailor biological responses through biphasic release
    Authors: Vivek A. Kumar, Nichole L. Taylor, Siyu Shi, Navindee C. Wickremasinghe, Rena N. D'Souza, Jeffrey D. Hartgerink
    Biomaterials
  27. 3D culture of murine neural stem cells on decellularized mouse brain sections
    Authors: Jorrit De Waele, Kristien Reekmans, Jasmijn Daans, Herman Goossens, Zwi Berneman, Peter Ponsaerts
    Biomaterials
  28. Transgenic kallikrein 5 mice reproduce major cutaneous and systemic hallmarks of Netherton syndrome
    Authors: Laetitia Furio, Simon de Veer, Madeleine Jaillet, Anais Briot, Aurelie Robin, Celine Deraison et al.
    Journal of Experimental Medicine
  29. Lipidoid Nanoparticles Containing PD-L1 siRNA Delivered In Vivo Enter Kupffer Cells and Enhance NK and CD8(+) T Cell-mediated Hepatic Antiviral Immunity
    Authors: Joseph S Dolina, Sun-Sang J Sung, Tatiana I Novobrantseva, Tuyen M Nguyen, Young S Hahn
    Molecular Therapy - Nucleic Acids
  30. Thymic stromal lymphopoietin (TSLP)-mediated dermal inflammation aggravates experimental asthma.
    Authors: Han H, Xu W, Headley MB
    Mucosal Immunol, 2012-02-22;5(3):342-51.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  31. Thymic stromal lymphopoietin contributes to myeloid hyperplasia and increased immunoglobulins, but not epidermal hyperplasia, in RabGEF1-deficient mice.
    Authors: Tsai M, Chen CC, Mukai K
    Am. J. Pathol., 2010-09-09;177(5):2411-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  32. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008-11-01;58(11):3510-9.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  33. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.
    Authors: Gaya M, Barral P, Burbage M et al.
    Cell
  34. Niche-Specific Factors Dynamically Regulate Sebaceous Gland Stem Cells in the Skin
    Authors: Natalia A. Veniaminova, Marina Grachtchouk, Owen J. Doane, Jamie K. Peterson, David A. Quigley, Madison V. Lull et al.
    Developmental Cell
  35. ACL injury reduces satellite cell abundance and promotes fibrogenic cell expansion within skeletal muscle
    Authors: Christopher S Fry
    J. Orthop. Res, 2017-01-15;0(0):.

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