Rat CXCL7/Thymus Chemokine-1 Antibody

Catalog #: AF1116 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1116
AF1116-SP

Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Rat CXCL7/Thymus Chemokine‑1 Antibody.

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Rat CXCL7/Thymus Chemokine-1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse CXCL7 is observed and less than 1% cross-reactivity with other chemokines tested.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1
Ile46-Ile107
Accession # NP_714943

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of rat thymus

Neutralization

Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.8-4.0 µg/mL in the presence of 0.04 µg/mL Recombinant Rat CXCL7/Thymus Chemokine‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Rat CXCL7/Thymus Chemokine‑1 Antibody. Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/Thymus Chemokine-1 (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1116). The ND₅₀ is typically 0.8-4.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Rat CXCL7 cDNA encodes a 111 amino acid residue precursor protein. Rat CXCL7 shares 72% amino acid sequence identity with mouse CXCL7 (accession number AAG36786). The recombinant rat CXCL7 produced by R&D Systems has been shown to chemoattract human CXCR2 transfected mouse BaF/3 cells, but not human CXCR1 transfected mouse BaF/3 cells.

¹rh6Ckine, rm6Ckine, rhBLC/BCA‑1, rmBLC, rhBRAK, rmBRAK, rmC10, rhCCL28, rmCCL28, rrCINC‑2a, rrCINC‑2b, rrCINC‑3, rhCKb8‑1, rvCMV UL146, rmCRG‑2, rhCTACK, rmCTACK, rhCXCL16, rmCXCL16, rhENA‑78, rhEotaxin, rmEotaxin, rhEotaxin‑2, rmEotaxin‑2, rhEotaxin‑3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP‑2, rmGCP‑2, rhGROa, rhGROb, rhGROg, rhHCC‑1, rhHCC‑4, rhI‑309, rhIL‑8, rpIL‑8, rcrIP‑10, rhIP‑10, rhI‑TAC, rmI‑TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rmLungkine, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP‑1, rhMCP‑2, rmMCP‑2, rhMCP‑3, rhMCP‑4, rmMCP‑5, rvMCV type 2, rhMDC, rmMDC, rhMIG, rmMIG, rcrMIP‑1a, rhMIP‑1a, rmMIP‑1a, rcrMIP‑1b, rhMIP‑1b, rmMIP‑1b, rhMIP‑1d, rmMIP‑1g, rmMIP‑2, rhMIP‑3a, rmMIP‑3a, rrMIP‑3a, rhMIP‑3b, rmMIP‑3b, rvMIP‑I, rvMIP‑II, rvMIP‑III, rhMPIF‑1, rhNAP‑2, rhPARC, rhPF4, rcrRANTES, rhRANTES, rmRANTES, rhSDF‑1a, rmSDF‑1a, rhSDF‑1b, rhTARC, rmTARC, rmTCA‑3, rhTeck, rmTeck

Entrez Gene IDs

57349 (Mouse); 246358 (Rat)

Alternate Names

Beta-TG;B-TG1;CTAP3;CTAPIII;CTAP-III;CXCL7;LA-PF4;LDGF;MDGF;NAP-2;PBP;Platelet basic protein;PPBP;SCYB7;TC1;TC2;TGB;TGB1;THBGB;THBGB1; Thymus Chemokine1; Thymus Chemokine-1