Rat Growth Hormone Antibody

Catalog # Availability Size / Price Qty
AF1566
AF1566-SP

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Growth Hormone in Rat Pituitary.
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Product Details
Citations (9)
FAQs
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Rat Growth Hormone Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Growth Hormone in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat Growth Hormone
Leu27-Phe216
Accession # P01244
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Rat Growth Hormone
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Growth Hormone antibody in Rat Pituitary by Immunohistochemistry (IHC-Fr). View Larger

Growth Hormone in Rat Pituitary. Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone

Growth Hormone, also named somatotropin, belongs to the somatotropin/prolactin superfamily. It is synthesized in the anterior pituitary gland. Growth Hormone regulates body growth by stimulating the secretion of IGF-I by liver and other tissues.

Entrez Gene IDs
2688 (Human)
Alternate Names
GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin

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Citations for Rat Growth Hormone Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Estradiol and progesterone regulate NUCB2/nesfatin-1 expression and function in GH3 pituitary cells and THESC endometrial cells
    Authors: Jinah Ha, Jungwoo Shin, Eunji Seok, Soohyun Kim, Sojung Sun, Hyunwon Yang
    Animal Cells and Systems
  2. Co-aggregation and secondary nucleation in the life cycle of human prolactin/galanin functional amyloids
    Authors: Debdeep Chatterjee, Reeba S Jacob, Soumik Ray, Ambuja Navalkar, Namrata Singh, Shinjinee Sengupta et al.
    eLife
  3. A new method for physical disector analyses of numbers and mean volumes of immunohistochemically labelled cells in paraffin sections
    Authors: I Hofmann, E Kemter, S Fiedler, N Theobalt, L Fonteyne, E Wolf, R Wanke, A Blutke
    Journal of neuroscience methods, 2021-07-01;361(0):109272.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  4. OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation
    Authors: M Yang, X Lin, F Segers, R Suganthan, GA Hildrestra, JE Rinholm, PA Aas, MML Sousa, S Holm, N Bolstad, D Warren, RK Berge, RF Johansen, A Yndestad, E Kristianse, A Klungland, L Luna, L Eide, B Halvorsen, P Aukrust, M Bjørås
    Cell Rep, 2020-03-24;30(12):4165-4178.e7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Chemokine CXCL14-like immunoreactivity in the &alphaMSH-producing cells and PRL-producing cells of the flat-tailed house gecko pituitary
    Authors: H Suzuki, T Yamamoto
    J. Vet. Med. Sci., 2020-02-10;82(4):408-413.
    Species: Gecko
    Sample Types: Whole Tissue
    Applications: IF
  6. Growth hormone receptor-deficient pigs resemble the pathophysiology of human Laron syndrome and reveal altered activation of signaling cascades in the liver
    Authors: Arne Hinrichs, Barbara Kessler, Mayuko Kurome, Andreas Blutke, Elisabeth Kemter, Maren Bernau et al.
    Molecular Metabolism
  7. Folliculostellate Cells Are Required for Laminin Release from Gonadotrophs in Rat Anterior Pituitary
    Authors: Takehiro Tsukada, Ken Fujiwara, Kotaro Horiguchi, Morio Azuma, Dini Ramadhani, Alimuddin Tofrizal et al.
    ACTA HISTOCHEMICA ET CYTOCHEMICA
  8. Wnt signaling in estrogen-induced lactotroph proliferation.
    Authors: Giles A, Madec F, Friedrichsen S, Featherstone K, Chambers T, Harper CV, Resch J, Brabant G, Davis JR
    J. Cell. Sci., 2011-01-18;124(0):540-7.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Traumatic brain injury causes long-term reduction in serum growth hormone and persistent astrocytosis in the cortico-hypothalamo-pituitary axis of adult male rats.
    Authors: Kasturi BS, Stein DG
    J. Neurotrauma, 2009-08-01;26(8):1315-24.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,Secondary

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