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Rat ICAM-1/CD54 Antibody

Catalog #: AF583
26 Citations 1 Review Datasheet / COA / SDS
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AF583
AF583-SP
Detection of Rat ICAM‑1/CD54 by Western Blot.
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Product Details
Citations (26)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat ICAM-1/CD54 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) ICAM-1 and 5% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM‑3, and rmICAM‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54
Gln28-Thr493
Accession # Q00238
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Rat ICAM-1 Fc Chimera (Catalog # 583-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat ICAM-1/CD54 antibody by Western Blot. View Larger

Detection of Rat ICAM‑1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ICAM‑1/CD54 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

ICAM‑1/CD54 in Rat Brain. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

References
  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Citations for Rat ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. The combination of IGF1 and FGF2 and the induction of excessive ocular growth and extreme myopia
    Authors: Eric R. Ritchey, Christopher P. Zelinka, Junhua Tang, Jun Liu, Andy J. Fischer
    Experimental Eye Research
  2. The generation of olfactory epithelial neurospheres in vitro predicts engraftment capacity following transplantation in vivo
    Authors: Richard C. Krolewski, Woochan Jang, James E. Schwob
    Experimental Neurology
  3. Sox2 and Pax6 Play Counteracting Roles in Regulating Neurogenesis within the Murine Olfactory Epithelium
    Authors: Adam I. Packard, Brian Lin, James E. Schwob
    PLOS ONE
  4. Therapeutic Effect of Platelet-Rich Plasma in Rat Spinal Cord Injuries
    Authors: Nan-Fu Chen, Chun-Sung Sung, Zhi-Hong Wen, Chun-Hong Chen, Chien-Wei Feng, Han-Chun Hung et al.
    Frontiers in Neuroscience
  5. Neurovascular protection by post-ischemic intravenous injections of the lipoxin A4 receptor agonist, BML-111, in a rat model of ischemic stroke
    Authors: Kimberly E. Hawkins, Kelly M. DeMars, Jonathan Singh, Changjun Yang, Henry S. Cho, Jan C. Frankowski et al.
    Journal of Neurochemistry
  6. Neuroinflammation Induced by Intracerebroventricular Injection of Microbial Neuraminidase
    Authors: Pablo Granados-Durán, María D. López-Ávalos, Jesús M. Grondona, María del Carmen Gómez-Roldán, Manuel Cifuentes, Margarita Pérez-Martín et al.
    Frontiers in Medicine
  7. Global expression profiling of globose basal cells and neurogenic progression within the olfactory epithelium
    Authors: Richard C. Krolewski, Adam Packard, James E. Schwob
    Journal of Comparative Neurology
  8. Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy
    Authors: M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang
    Journal of Cellular and Molecular Medicine, 2022-01-11;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. A Group of Olfactory Receptor Alleles that Encode Full Length Proteins are Down-Regulated as Olfactory Sensory Neurons Mature
    Authors: RC Krolewski, B Lin, S Stampfer, A Packard, JE Schwob
    Sci Rep, 2020-02-04;10(1):1781.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Effects of the Emitted Light Spectrum of Liquid Crystal Displays on Light-Induced Retinal Photoreceptor Cell Damage
    Authors: CW Lin, CM Yang, CH Yang
    Int J Mol Sci, 2019-05-10;20(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice
    Authors: SA Bloomer, KE Wellen, GC Henderson
    Lipids Health Dis, 2017-12-13;16(1):243.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis
    Authors: J Huang, S Han, Q Sun, Y Zhao, J Liu, X Yuan, W Mao, B Peng, W Liu, J Yin, X He
    Cell Biosci, 2017-06-07;7(0):31.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  13. Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs
    Authors: G Hu, J Wang, D Hong, T Zhang, H Duan, X Mu, Z Yang
    BMC Complement Altern Med, 2017-01-11;17(1):38.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-19;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. Sustained elevation of NF-?B activity sensitizes offspring of maternal inflammation to hypertension via impairing PGC-1? recovery
    Sci Rep, 2016-09-12;6(0):32642.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
    Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
    PLoS ONE, 2016-01-14;11(1):e0146438.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
    Authors: Hanusch C, Nowak K, Gill IS, Torlitz P, Rafat N, Mueller AM, Van Ackern KC, Yard B, Beck GC
    Clin. Exp. Immunol., 2007-05-22;149(2):364-71.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  18. Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
    Authors: Sun W, Gerhardinger C, Dagher Z, Hoehn T, Lorenzi M
    Diabetes, 2005-12-01;54(12):3418-26.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  19. Is Iba-1 protein expression a sensitive marker for microglia activation in experimental diabetic retinopathy?
    Authors: Shi FJ, Xie H, Zhang CY et al.
    International journal of ophthalmology
  20. Detrimental role of the EP1 prostanoid receptor in blood-brain barrier damage following experimental ischemic stroke
    Authors: Jan C. Frankowski, Kelly M. DeMars, Abdullah S. Ahmad, Kimberly E. Hawkins, Changjun Yang, Jenna L. Leclerc et al.
    Scientific Reports
  21. Renal Tubular Cell-Derived Extracellular Vesicles Accelerate the Recovery of Established Renal Ischemia Reperfusion Injury
    Authors: Jesus H. Dominguez, Yunlong Liu, Hongyu Gao, James M. Dominguez, Danhui Xie, K. J. Kelly
    Journal of the American Society of Nephrology
  22. Modulatory effects of vagal stimulation on neurophysiological parameters and the cellular immune response in the rat brain during systemic inflammation
    Authors: Hanna Schweighöfer, Christoph Rummel, Joachim Roth, Bernhard Rosengarten
    Intensive Care Medicine Experimental
  23. Differential expression of components of the retinoic acid signaling pathway in the adult mouse olfactory epithelium
    Authors: Carolyn E. Peluso, Woochan Jang, Ursula C. Dräger, James E. Schwob
    The Journal of Comparative Neurology
  24. Expression of Pax6 and Sox2 in adult olfactory epithelium
    Authors: Zhen Guo, Adam Packard, Richard C. Krolewski, Margaret T. Harris, Glen L. Manglapus, James E. Schwob
    The Journal of Comparative Neurology
  25. The role and mechanism of the annexin A1 peptide Ac2-26 in rats with cardiopulmonary bypass lung injury
    Authors: Xu J, Yu C, Luo J, et al.
    Basic & clinical pharmacology & toxicology
  26. Spinal cord injury and its underlying mechanism in rats with temporal lobe epilepsy
    Authors: Jinjie Liu, Zanhua Liu, Guoliang Liu, Kai Gao, Hengjie Zhou, Yongbo Zhao et al.
    Experimental and Therapeutic Medicine

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Rat ICAM-1/CD54 Antibody
By Gaia Brezzo on 09/06/2016
Application: IHC Sample Tested: Brain tissue Species: Rat
Immunohistochemistry Rat ICAM-1/CD54 Antibody AF583