Rat PGD2 Synthase/PTGDS Antibody

Catalog #: AF5000 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5000
AF5000-SP

Detection of Rat PGD2 Synthase/PTGDS by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat PGD2 Synthase/PTGDS Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat PGD2 Synthase/PTGDS in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat PGD2 Synthase/PTGDS
Gln25-Glu189
Accession # P22057

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat PGD2 Synthase/PTGDS by Western Blot. Western blot shows lysates rat brain and spinal cord tissue. PVDF membrane was probed with 1 µg mL of Goat Anti-Rat PGD2 Synthase PTGDS Antigen Affinity-purified Polyclonal (Catalog # AF5000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PGD2 Synthase/PTGDS at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGD2 Synthase/PTGDS

PGD2 Synthase (prostaglandin D2 synthase; also PG-H2 D-isomerase) is a 26 kDa, monomeric member of the lipocalin family of proteins. It is expressed by neurons and astrocytes, retinal pigment epithelium and Sertoli cells. It catalyzes the conversion of PGH₂ to PGD₂, thereby influencing sleep and body temperature. It also transports retinoic acid. The rat PGD2 precursor is 189 amino acids (aa) in length. It contains a signal sequence (aa 1‑24) plus a lipocalin region that serves both as a catalytic site and hydrophobic molecule transporter (aa 40‑187). PGD2 is both intracellular and secreted. When secreted, PGD2 is called beta -Trace. One splice variant is likely intracellular and shows an 11 aa substitution for the first 52 amino acids. Over aa 25‑189, rat PGD2 shares 72% and 88% aa identity with human and mouse PGD2, respectively.

Long Name

Prostaglandin D2 Synthase

Entrez Gene IDs

5730 (Human); 25526 (Rat)

Alternate Names

Beta-trace protein; Cerebrin-28; EC 5.3.99.2; Glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; Lipocalin-type prostaglandin-D synthase; L-PGDS; PDS; PGD2 Synthase; PGD2; PGDS2; PGDSLPGDS; PH2DISO; prostaglandin D synthase; prostaglandin D2 synthase (21kD, brain); prostaglandin D2 synthase 21kDa (brain); Prostaglandin-D2 synthase; prostaglandin-H2 D-isomerase; PTGDS