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Human beta IG-H3 Antibody

Catalog #: AF2935
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2935
AF2935-SP
Product Details
Citations (6)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta IG-H3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta IG-H3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse beta IG-H3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta IG-H3
Gly24-His683
Accession # Q15582
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human beta IG-H3 (Catalog # 3409-BG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta IG-H3

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1‑4). The human beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (1). Human beta IG-H3 shares 91% and 93% aa sequence identity with mouse and porcine beta IG-H3, respectively. beta IG-H3 is expressed as a 75 kDa protein with no post‑translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5,6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 binding to matrix Fibronectin, Collagen I, and Collagen VI (3, 8‑10) in addition to cell expressed Integrins alpha V beta 3, alpha V beta 5, and alpha 3 beta 1 (7, 8, 11, 12). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 11, 13). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8,11). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (12, 13). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (14). beta IG-H3 is downregulated in many cancers (4, 15) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 15).

References
  1. Skonier, J. et al. (1992) DNA Cell Biol. 11:511.
  2. Skonier, J. et al. (1994) DNA Cell Biol. 13:571.
  3. Lee, S-H. et al. (2003) Kidney Int. 64:1012.
  4. Zhao, Y.L. et al. (2002) Oncogene 21:7471.
  5. Andersen, R.B. et al. (2004) Biochemistry 43:16374.
  6. Kim, J-E. et al. (2003) Oncogene 22:2045.
  7. Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.
  8. Thapa, N. et al. (2005) Bone 36:232.
  9. Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.
  10. Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.
  11. Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.
  12. Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.
  13. Cao, W, et al. (2006) Blood 107:2777. 
  14. Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.
  15. Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.
Long Name
TGF-beta Induced Gene H3
Entrez Gene IDs
7045 (Human); 21810 (Mouse)
Alternate Names
beta IGH3; beta IG-H3; BIGH3EBMD; CDB1; CDG2; CDGG1transforming growth factor, beta-induced, 68kD; CSD; CSD1; CSD2; CSD3; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBIP; transforming growth factor, beta-induced, 68kDa; transforming growth factor-beta-induced protein ig-h3

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Citations for Human beta IG-H3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells*
    Authors: Dean E. Hammond, J. Dinesh Kumar, Lorna Raymond, Deborah M. Simpson, Robert J. Beynon, Graham J. Dockray et al.
    Molecular & Cellular Proteomics
  2. Extremely varied phenotypes in granular corneal dystrophy type 2 heterozygotes
    Authors: Kyung Eun Han, Seung-il Choi, Woo Suk Chung, Se Hwan Jung, Nicholas Katsanis, Tae-im Kim et al.
    Mol. Vis
  3. Impaired autophagy and delayed autophagic clearance of transforming growth factor beta-induced protein (TGFBI) in granular corneal dystrophy type 2.
    Authors: Choi S, Kim B, Dadakhujaev S, Oh J, Kim T, Kim J, Kim E
    Autophagy, 2012-09-20;8(12):1782-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Decreased catalase expression and increased susceptibility to oxidative stress in primary cultured corneal fibroblasts from patients with granular corneal dystrophy type II.
    Authors: Choi SI, Kim TI, Kim KS, Kim BY, Ahn SY, Cho HJ, Lee HK, Cho HS, Kim EK
    Am. J. Pathol., 2009-06-04;175(1):248-61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts
    Authors: Yong-Sun Maeng, Ga-Hyun Lee, Seung-Il Choi, Kyu Seo Kim, Eung Kweon Kim
    BMC Medical Genomics
  6. The effect of differentiation and TGF? on mitochondrial respiration and mitochondrial enzyme abundance in cultured primary human skeletal muscle cells
    Authors: C Hoffmann, S Höckele, L Kappler, M Hrab? de A, HU Häring, C Weigert
    Sci Rep, 2018-01-15;8(1):737.

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