Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 471916
View all Bradykinin RB2/BDKRB2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Bradykinin RB2/BDKRB2
Met1-Gln391
Accession # P30411

Specificity

Detects human Bradykinin RB2/BDKRB2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Bradykinin RB2/BDKRB2 Antibody

Detection of Bradykinin RB2/BDKRB2 antibody in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP antibody by Flow Cytometry.

Detection of Bradykinin RB2/BDKRB2 in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human Bradykinin RB2/BDKRB2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Bradykinin RB2/BDKRB2 antibody in Human Stomach by Immunohistochemistry (IHC-P).

Bradykinin RB2/BDKRB2 in Human Stomach.

Bradykinin RB2/BDKRB2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Bradykinin RB2/BDKRB2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bradykinin RB2/BDKRB2

Bradykinin RB2 (BDKRB2) is a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. BDKRB2 expression is widespread in normal smooth muscle tissue and neurons. BDKRB2 associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. BDKRB2 forms a complex with PECAM1 and GNAQ and interacts with PECAM1. Aging cardiac endothelial cells gradually lose their capacity to express BDKRB2. This loss appears to be parallel with a loss of the angiogenic potential of the aging cells.

Long Name

Bradykinin Receptor B2

Alternate Names

B2R, BDKRB2, BKR2, BradykininRB2, BRB2

Entrez Gene IDs

624 (Human); 12062 (Mouse); 25245 (Rat)

Gene Symbol

BDKRB2

UniProt

P30411

Additional Bradykinin RB2/BDKRB2 Products

Product Documents for Human Bradykinin RB2/BDKRB2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Bradykinin RB2/BDKRB2 Antibody

For research use only

Citations for Human Bradykinin RB2/BDKRB2 Antibody

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Protocols

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