Human Bradykinin RB2/BDKRB2 Antibody

Catalog #: MAB47251 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB47251-100
MAB47251-SP

Detection of Bradykinin RB2/BDKRB2 in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Bradykinin RB2/BDKRB2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Bradykinin RB2/BDKRB2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 471916

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Bradykinin RB2/BDKRB2
Met1-Gln391
Accession # P30411

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Bradykinin RB2/BDKRB2 antibody in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP antibody by Flow Cytometry.

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Detection of Bradykinin RB2/BDKRB2 in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Bradykinin RB2/BDKRB2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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Bradykinin RB2/BDKRB2 in Human Stomach. Bradykinin RB2/BDKRB2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bradykinin RB2/BDKRB2

Bradykinin RB2 (BDKRB2) is a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. BDKRB2 expression is widespread in normal smooth muscle tissue and neurons. BDKRB2 associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. BDKRB2 forms a complex with PECAM1 and GNAQ and interacts with PECAM1. Aging cardiac endothelial cells gradually lose their capacity to express BDKRB2. This loss appears to be parallel with a loss of the angiogenic potential of the aging cells.

Long Name

Bradykinin Receptor B2

Entrez Gene IDs

624 (Human); 12062 (Mouse); 25245 (Rat)

Alternate Names

B2 bradykinin receptor; B2R; BDKRB2; BK-2 receptor; BK2; BK-2; BKR2; Bradykinin RB2; bradykinin receptor B2; BradykininRB2; BRB2; DKFZp686O088