Human EDAR Antibody

Catalog #: AF157
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF157
AF157-SP

All EDAR Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EDAR Antibody Summary

Species Reactivity

Human

Specificity

Detects human EDAR in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhCD40, rhDR6, rhTAJ, rhTNF RI, rhTNF RII, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGFR, rhOPG and rhRANK is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EDAR
Glu27-Ile189
Accession # Q9UNE0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human EDAR Fc Chimera (Catalog # 157-ER)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 4-16 µg/mL of this antibody will block 50% of the binding of 10 μg/mL of Recombinant Human EDA-A1 (Catalog # 3944-ED) to immobilized Recombinant Human EDAR Fc Chimera (Catalog # 157-ER) coated at 1 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDAR

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region; the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Human EDAR is a 488 amino acid (aa) protein with a predicted 26 aa signal, a 163 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The human and mouse EDAR homologs share 91% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

References

Headon, D.J. and P.A. Overbeek (1999) Nat.Genet. 22:370.
Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
Yan, M. et al. (2000) Science 290:523.

Long Name

Ectodysplasin Receptor

Entrez Gene IDs

10913 (Human); 13608 (Mouse); 102120238 (Cynomolgus Monkey)

Alternate Names

Anhidrotic ectodysplasin receptor 1; DLED3; Ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; ectodysplasin A receptor; Ectodysplasin-A receptor; ED1R; ED5; EDA1R; EDA3EDA-A1 receptor; EDA-A1R; EDAR; FLJ94390; HRM1; mouse, homolog of; tumor necrosis factor receptor superfamily member EDAR

Product Datasheets

Product Datasheet

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Citation for Human EDAR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1