Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 226706
View all Ghrelin/Obestatin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human Ghrelin/Obestatin
Accession # Q9UBU3

Specificity

Detects human Ghrelin/Obestatin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Ghrelin/Obestatin Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Ghrelin/Obestatin Monoclonal Antibody (Catalog # MAB81492).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ghrelin/Obestatin

Mature Ghrelin peptides are the result of the clavage of the Ghrelin/Obestatin Prepropeptide, a 117 aa precursor peptide that is processed into three chains, Ghrelin-27 (aa 24-50), Ghrelin-28 (aa24-51) and Obestatin (aa76-98).  The prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases.  Mature Ghrelin is acylated with an N-octanoyl group on serine 3 which is required for receptor binding.  Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa.  Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs.  Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.

Alternate Names

appetite-regulating hormone, ghrelin, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRPgrowth hormone secretagogue receptor ligand, obestatin, Protein M46

Entrez Gene IDs

51738 (Human)

Gene Symbol

GHRL

UniProt

Q9UBU3 (Human)

Additional Ghrelin/Obestatin Products

Product Documents for Human Ghrelin/Obestatin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Ghrelin/Obestatin Antibody

For research use only

Related Research Areas

Nutrition and Appetite
Obesity

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