Human HAND1 Antibody

Catalog #: AF3168
13 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3168
AF3168-SP

Detection of Human HAND1 by Western Blot.

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All HAND1 Antibodies

Product Details

Citations (13)

Supplemental Products

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAND1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HAND1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HAND2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HAND1
Met1-Gln215
Accession # O96004

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

Immersion fixed SH‑SY5Y human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HAND1 by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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HAND1 in SH‑SY5Y Human Cell Line. HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAND1

HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.

Long Name

Heart And Neural Crest Derivatives Expressed 1

Entrez Gene IDs

9421 (Human); 15110 (Mouse); 59112 (Rat)

Alternate Names

BHLHA27; bHLHa27heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1; HAND1; heart and neural crest derivatives expressed 1; Hxt; Thing1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human HAND1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Characterization of an induced pluripotent stem cell line NCHi011-A from a 23-year-old female with Alagille Syndrome harboring a heterozygous JAG1 pathogenic variant
Authors: Stanberry, I;Cunningham, D;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B;
Stem cell research
Species: Human
Sample Types: Whole Cells
Applications: ICC
Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells
Authors: Marina Gattiglio, Michelle Protzek, Christian Schröter
Biology Open
Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene
Authors: Cunningham, D;Stanberry, I;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B;
Stem cell research
Species: Human
Sample Types: Whole Cells
Applications: ICC
Origin and segregation of the human germline
Authors: Aracely Castillo-Venzor, Christopher A Penfold, Michael D Morgan, Walfred WC Tang, Toshihiro Kobayashi, Frederick CK Wong et al.
Life Science Alliance
Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay
Authors: Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al.
Cell Reports Methods
The time integral of BMP signaling determines fate in a stem cell model for early human development
Authors: S Teague, G Primavera, B Chen, E Freeburne, H Khan, K Jo, C Johnson, I Heemskerk
bioRxiv : the preprint server for biology, 2023-04-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome
Authors: EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova
Stem Cell Research, 2022-12-30;67(0):103012.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
Authors: H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride
Stem Cell Research, 2022-10-31;65(0):102958.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model
Authors: M Ohgushi, N Taniyama, A Vandenbon, M Eiraku
Cell Reports, 2022-06-21;39(12):110973.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
A stress-reduced passaging technique improves the viability of human pluripotent cells
Authors: Kazutoshi Takahashi, Chikako Okubo, Michiko Nakamura, Mio Iwasaki, Yuka Kawahara, Tsuyoshi Tabata et al.
Cell Reports Methods

Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis
Authors: A Mehta, P Lu, BV Taylor, J Charleswor, AL Cook, KP Burdon, AW Hewitt, KM Young
Stem Cell Research, 2021-10-12;57(0):102568.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC

Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development
Authors: M Zheng, S Erhardt, D Ai, J Wang
International Journal of Molecular Sciences, 2021-09-11;22(18):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids
Authors: S Chhabra, L Liu, R Goh, X Kong, A Warmflash
PLoS Biol., 2019-10-15;17(10):e3000498.
Species: Human
Sample Types: Whole Cells
Applications: ICC