Human HAX-1 Antibody

Catalog #: AF5458
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5458
AF5458-SP

Detection of Human HAX‑1 by Western Blot.

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All HAX-1 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAX-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HAX-1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HAX-1
Ser2-Leu261
Accession # O00165

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HAX‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HAX-1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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HAX‑1 in HepG2 Human Cell Line. HAX-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAX-1

HAX-1 (HCLS1-associated protein X-1; also HS1-binding protein1) is a 34‑35 kDa intracellular protein with structural similarity to Bcl-2 family proteins. It is ubiquitously expressed, and participates in a variety of cell-specific functions. HAX‑1 blocks cardiomyocyte apoptosis via caspase‑9 inhibition, downregulates conjugated bile acid secretion via BSEP binding, and via PKD2, transmitts integrin signals to the ER. Human HAX-1 is 279 amino acids (aa) in length and contains two Bcl-2 homology regions (aa 37‑56 and 74‑89), a PEST domain (aa 104‑117) and a potential transmembrane segment (aa 261‑279). HS-1 is found mostly in the mitochondria. HAX-1 is also cleaved by caspase-3 at Asp127. Over aa 2‑261, human HAX-1 shares 81% aa identity with mouse HAX-1.

Long Name

HCLS1 associated protein X-1

Entrez Gene IDs

10456 (Human); 23897 (Mouse); 291202 (Rat)

Alternate Names

FLJ17042; FLJ93803; HAX1; HAX-1; HCLS1 (and PKD2) associated protein; HCLS1 associated protein X-1; HCLS1-associated protein X-1; HCLSBP1; HS1 binding protein; HS1-associating protein X-1; HS1-binding protein 1; HS1BP1; HS1BP1FLJ18492; HSP1BP-1; SCN3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human HAX-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The type III secretion system effector EspO of enterohaemorrhagic Escherichia coli inhibits apoptosis through an interaction with HAX ‐1
Authors: Sharanya Chatterjee, Sujinna Lekmeechai, Nicolas Constantinou, Ewa A. Grzybowska, Zuzanna Kozik, Jyoti S. Choudhary et al.
Cellular Microbiology
PELO negatively regulates HER receptor signalling and metastasis.
Authors: Pedersen K, Canals F, Prat A, Tabernero J, Arribas J
Oncogene, 2013-02-25;33(9):1190-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot