Human Ku70/XRCC6 Antibody

Catalog #: AF5597 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5597
AF5597-SP

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Detection of Human Ku70/XRCC6 by Western Blot.

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All Ku70/XRCC6 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ku70/XRCC6 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Ku70 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Ku70
Asn405-Lys556
Accession # P12956

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Ku70/XRCC6 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human Ku70/XRCC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5597) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Ku70/XRCC6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ku70/XRCC6

Ku70 (Lupus Ku autoantigen protein 70; also ATP-dependent DNA helicase 2 subunit 1) is a ubiquitous 70‑75 kDa nuclear protein that serves as a component of the DNA-dependent protein kinase complex DNA-PK. In particular, it heterodimerizes with Ku80 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku70 is 609 amino acids (aa) in length. It contains a Ku core domain (aa 266‑529), a DNA binding motif (aa 268‑274), an NLS (aa 539‑556) and a C-terminal SAP domain (aa 573‑607). There is one potential splice form that shows a deletion of aa 66‑106. Over aa 405‑556, Ku70 shares 83% aa identity with mouse Ku70.

Long Name

Lupus Ku Autoantigen Protein 70

Entrez Gene IDs

2547 (Human)

Alternate Names

5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; D22S731; EC 3.6.4.-; EC 4.2.99.-; G22P1; G22P1Ku70; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; ML8; ML8,70 kDa subunit; thyroid-lupus autoantigen p70; Thyroid-lupus autoantigen; TLAA; TLAAD22S671; X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6; X-ray repair cross-complementing protein 6; XRCC6