Human LARG Antibody

Catalog #: AF4737
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4737
AF4737-SP

Detection of Human LARG by Western Blot.

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Citations (2)

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Human LARG Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human LARG in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LARG
Ile154-Leu575
Accession # Q9NZN5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LARG by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human LARG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LARG at approximately 200 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LARG

LARG (Leukemia-associated RhoGEF, also known as Rho guanine nucleotide exchange factor 12) is a 173 kDa member of the DH‑domain containing RhoGEF family of molecules. It is ubiquitously expressed, and known to interact with semaphorin-activated B class plexins, thereby activating Rho-dependent pathways. Human LARG is 1544 amino acids (aa) in length. It contains one PZD domain that binds to plexin (aa 72‑151), an RGSL region (aa 368‑558), and a RhoGEF domain (aa 788‑975). There are at least two potential splice forms. One shows a deletion of aa 48‑66 with an 8 aa poly-Lys substitution for the C-terminal 960 amino acids. A second shows an insertion of 9 aa between Thr1093 and Asp1094, accompanied by a 6 aa substitution for the C-terminal 131 amino acids. Over aa 154‑575, human LARG is 90% aa identical to mouse LARG.

Long Name

Leukemia-associated RhoGEF

Entrez Gene IDs

23365 (Human); 69632 (Mouse); 367072 (Rat)

Alternate Names

ARHGC; ARHGEF12; DKFZp686O2372; KIAA0382PRO2792; LARG; LARGleukemia-associated rho guanine nucleotide exchange factor; Leukemia-associated RhoGEF; Rho guanine nucleotide exchange factor (GEF) 12; rho guanine nucleotide exchange factor 12

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human LARG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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