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Human LDLR Antibody

Catalog #: AF2148
39 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2148
AF2148-SP
LDL R in HepG2 Human Cell Line.
4 Images
Product Details
Citations (39)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LDLR Antibody Summary

Species Reactivity
Human
Specificity
Detects human LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse LDL R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LDL R
Asp193-Arg788
Accession # P01130
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LDL R (Catalog # 2148-LD)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Human LDL R (Catalog # 2148-LD) to immobilized human low density lipoprotein coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.
 
Immunocytochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry LDL R antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

LDL R in HepG2 Human Cell Line. LDL R was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2148) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry LDL R antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

LDL R in Human Liver. LDL R was detected in formalin fixed paraffin-embedded sections of human liver using Goat Anti-Human LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockdown Validated Detection of Human LDLR by Knockdown Validated View Larger

Detection of Human LDLR by Knockdown Validated APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.(A and B) Western blot showing APLP2, PCSK9, or Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.) in the absence or presence of 5F6 Fab or 12E3 Fab, as indicated. (B) Quantification of (A); shown as average APLP2 normalized to PCSK9 of 3 independent experiments with SEM. (C and D) J16 coIPs of PCSK9 from Neg or LDLR siRNA treated HepG2 cells with IC control, as indicated. (D) Quantification of (C); shown as average APLP2 normalized to PCSK9 from 3 independent experiments with SEM. (E, F, and G) Western blot of LDLR, APOER2, or TFNR in siRNA treated cells following treatment with PCSK9 at 0, 20, 50, or 100 μg/ml. (F) LDLR levels from (E) quantified as percent LDLR degradation of untreated cells and normalized to Neg siRNA samples. Shown as average with SEM from 4 independent experiments. (G) Same as F, but measuring APOER2 levels. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25905719), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown Validated Detection of Human LDLR by Knockdown Validated View Larger

Detection of Human LDLR by Knockdown Validated APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.(A and B) Western blot showing APLP2, PCSK9, or Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.) in the absence or presence of 5F6 Fab or 12E3 Fab, as indicated. (B) Quantification of (A); shown as average APLP2 normalized to PCSK9 of 3 independent experiments with SEM. (C and D) J16 coIPs of PCSK9 from Neg or LDLR siRNA treated HepG2 cells with IC control, as indicated. (D) Quantification of (C); shown as average APLP2 normalized to PCSK9 from 3 independent experiments with SEM. (E, F, and G) Western blot of LDLR, APOER2, or TFNR in siRNA treated cells following treatment with PCSK9 at 0, 20, 50, or 100 μg/ml. (F) LDLR levels from (E) quantified as percent LDLR degradation of untreated cells and normalized to Neg siRNA samples. Shown as average with SEM from 4 independent experiments. (G) Same as F, but measuring APOER2 levels. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25905719), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LDLR

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors. This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell‑surface molecules, expanding their range of activities. Human LDL R is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail. The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr‑rich region that serves as a carbohydrate attachment point. There is extensive O‑linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120 ‑ 160 kDa. Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits. The extracellular region of human LDL R is 51% aa identical to the extracellular region of human VLDL R, and 79% aa identical to the extracellular region of mouse LDL R. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL. It is responsible for clearing 70% of plasma LDL in liver. Mutations in the LDL R gene cause the autosomal dominant disorder, familial hypercholesterolemia.

Long Name
Low Density Lipoprotein Receptor
Entrez Gene IDs
3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)
Alternate Names
FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor

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Citations for Human LDLR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. A virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in non-human primates
    Authors: Alexandra Fowler, Koen K. A. Van Rompay, Maureen Sampson, Javier Leo, Jennifer K. Watanabe, Jodie L. Usachenko et al.
    NPJ Vaccines
  2. Proteolysis of the low density lipoprotein receptor by bone morphogenetic protein-1 regulates cellular cholesterol uptake
    Authors: Sreemoti Banerjee, Robert J. Andrew, Christopher J. Duff, Kate Fisher, Carolyn D. Jackson, Catherine B. Lawrence et al.
    Scientific Reports
  3. LDL receptor-peptide conjugate as in vivo tool for specific targeting of pancreatic ductal adenocarcinoma
    Authors: Angélina Acier, Magali Godard, Fanny Gassiot, Pascal Finetti, Marion Rubis, Jonathan Nowak et al.
    Communications Biology
  4. Combined Effects of Rosuvastatin and Exercise on Gene Expression of Key Molecules Involved in Cholesterol Metabolism in Ovariectomized Rats
    Authors: Emilienne Tudor Ngo Sock, Gaétan Mayer, Jean-Marc Lavoie
    PLOS ONE
  5. Trafficking Dynamics of PCSK9-Induced LDLR Degradation: Focus on Human PCSK9 Mutations and C-Terminal Domain
    Authors: Steve Poirier, Hocine Ait Hamouda, Louis Villeneuve, Annie Demers, Gaétan Mayer
    PLOS ONE
  6. Production and characterization of high-titer serum-free cell culture grown hepatitis C virus particles of genotype 1–6
    Authors: Christian K. Mathiesen, Tanja B. Jensen, Jannick Prentoe, Henrik Krarup, Alfredo Nicosia, Mansun Law et al.
    Virology
  7. Lipoproteins act as vehicles for lipid antigen delivery and activation of invariant natural killer T cells
    Authors: Suzanne E. Engelen, Francesca A. Ververs, Angela Markovska, B. Christoffer Lagerholm, Jordan M. Kraaijenhof, Laura I.E. Yousif et al.
    JCI Insight
  8. Altered cell function and increased replication of rhinoviruses and EV-D68 in airway epithelia of asthma patients
    Authors: Manel Essaidi-Laziosi, Léna Royston, Bernadett Boda, Francisco Javier Pérez-Rodriguez, Isabelle Piuz, Nicolas Hulo et al.
    Frontiers in Microbiology
  9. The low-density lipoprotein receptor and apolipoprotein E associated with CCHFV particles mediate CCHFV entry into cells
    Authors: Ritter, M;Canus, L;Gautam, A;Vallet, T;Zhong, L;Lalande, A;Boson, B;Gandhi, A;Bodoirat, S;Burlaud-Gaillard, J;Freitas, N;Roingeard, P;Barr, JN;Lotteau, V;Legros, V;Mathieu, C;Cosset, FL;Denolly, S;
    Nature communications
    Species: Human, Primate - Cercopithecus aethiops (African Green Monkey)
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot, Neutralization, Immunocytochemistry
  10. Insights into PCSK9-LDLR Regulation and Trafficking via the Differential Functions of MHC-I Proteins HFE and HLA-C
    Authors: Mikaeeli, S;Ben Djoudi Ouadda, A;Evagelidis, A;Essalmani, R;Ramos, OHP;Fruchart-Gaillard, C;Seidah, NG;
    Cells
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, Immunocytochemistry
  11. A high-cholesterol diet promotes the intravasation of breast tumor cells through an LDL-LDLR axis
    Authors: Magalhães, A;Cesário, V;Coutinho, D;Matias, I;Domingues, G;Pinheiro, C;Serafim, T;Dias, S;
    Scientific reports
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  12. iPSC-Derived Endothelial Cells Reveal LDLR Dysfunction and Dysregulated Gene Expression Profiles in Familial Hypercholesterolemia
    Authors: Zakharova, IS;Shevchenko, AI;Arssan, MA;Sleptcov, AA;Nazarenko, MS;Zarubin, AA;Zheltysheva, NV;Shevchenko, VA;Tmoyan, NA;Saaya, SB;Ezhov, MV;Kukharchuk, VV;Parfyonova, YV;Zakian, SM;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Identification of PCPE-2 as the endogenous specific inhibitor of human BMP-1/tolloid-like proteinases
    Authors: Vadon-Le Goff, S;Tessier, A;Napoli, M;Dieryckx, C;Bauer, J;Dussoyer, M;Lagoutte, P;Peyronnel, C;Essayan, L;Kleiser, S;Tueni, N;Bettler, E;Mariano, N;Errazuriz-Cerda, E;Fruchart Gaillard, C;Ruggiero, F;Becker-Pauly, C;Allain, JM;Bruckner-Tuderman, L;Nyström, A;Moali, C;
    Nature communications
    Species: Mouse, Human
    Sample Types: Protein
    Applications: Western Blot
  14. A Virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in Non-Human Primates
    Authors: Fowler, A;Van Rompay, KKA;Sampson, M;Leo, J;Watanabe, JK;Usachenko, JL;Immareddy, R;Lovato, DM;Schiller, JT;Remaley, AT;Chackerian, B;
    bioRxiv : the preprint server for biology
    Species: Primate - Macaca fascicularis (Cynomolgus Macaque)
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Targeting UGCG overcomes resistance to lysosomal autophagy inhibition
    Authors: V Jain, SL Harper, AM Versace, D Fingerman, GS Brown, M Bhardwaj, MAS Crissey, AR Goldman, G Ruthel, Q Liu, A Zivkovic, H Stark, M Herlyn, PA Gimotty, DW Speicher, RK Amaravadi
    Cancer Discovery, 2023-02-06;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  16. Role of PAI-1 in hepatic steatosis and dyslipidemia
    Authors: JA Levine, C Oleaga, M Eren, AP Amaral, M Shang, E Lux, SS Khan, SJ Shah, Y Omura, N Pamir, J Hay, G Barish, T Miyata, H Tavori, S Fazio, DE Vaughan
    Scientific Reports, 2021-01-11;11(1):430.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis
    Authors: L Huang, KL Chambliss, X Gao, IS Yuhanna, E Behling-Ke, S Bergaya, M Ahmed, P Michaely, K Luby-Phelp, A Darehshour, L Xu, EA Fisher, WP Ge, C Mineo, PW Shaul
    Nature, 2019-04-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. HIV-induced neuroinflammation: impact of PAR1 and PAR2 processing by Furin
    Authors: V Sachan, R Lodge, K Mihara, J Hamelin, C Power, BB Gelman, MD Hollenberg, ÉA Cohen, NG Seidah
    Cell Death Differ., 2019-01-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis
    Authors: N Volkmar, ML Thezenas, SM Louie, S Juszkiewic, DK Nomura, RS Hegde, BM Kessler, JC Christians
    J. Cell. Sci., 2019-01-16;132(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor
    Authors: PM Grin, DJ Dwivedi, KM Chathely, BL Trigatti, A Prat, NG Seidah, PC Liaw, AE Fox-Robich
    Sci Rep, 2018-07-12;8(1):10496.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  21. 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
    Authors: X Li, J Guo, N Liang, X Jiang, Y Song, S Ou, Y Hu, R Jiao, W Bai
    Front Pharmacol, 2018-02-27;9(0):159.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Antisense oligonucleotides targeting translation inhibitory elements in 5' UTRs can selectively increase protein levels
    Authors: XH Liang, H Sun, W Shen, S Wang, J Yao, MT Migawa, HH Bui, SS Damle, S Riney, MJ Graham, RM Crooke, ST Crooke
    Nucleic Acids Res., 2017-09-19;45(16):9528-9546.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
    Authors: SA Felt, GN Droby, VZ Grdzelishv
    J. Virol., 2017-07-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Applying antibody-sensitive hypervariable region 1-deleted hepatitis C virus to the study of escape pathways of neutralizing human monoclonal antibody AR5A
    Authors: R Velázquez-, M Law, J Bukh, J Prentoe
    PLoS Pathog, 2017-02-23;13(2):e1006214.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. A novel mechanism causing familial hypercholesterolemia: The proprotein convertase subtilisin/kexin type 9-resistant Arg410Ser LDL receptor mutation
    Authors: Delia Susan-Resi
    J. Biol. Chem, 2016-12-20;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  26. Identification of a Chrysanthemic Ester as an Apolipoprotein E Inducer in Astrocytes
    PLoS ONE, 2016-09-06;11(9):e0162384.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
    Authors: Elodie Weider
    J Biol Chem, 2016-06-08;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes
    PLoS ONE, 2016-04-26;11(4):e0154498.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
    Authors: Le Q, Blanchet M, Seidah N, Labonte P
    J Biol Chem, 2015-07-20;290(38):23385-400.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Immunoprecipitation
  30. Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
    Authors: DeVay R, Yamamoto L, Shelton D, Liang H
    PLoS ONE, 2015-04-23;10(4):e0125127.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  31. Differential complement activation pathways promote C3b deposition on native and acetylated LDL thereby inducing lipoprotein binding to the complement receptor 1.
    Authors: Klop B, van der Pol P, van Bruggen R, Wang Y, de Vries M, van Santen S, O'Flynn J, van de Geijn G, Njo T, Janssen H, de Man P, Jukema J, Rabelink T, Rensen P, van Kooten C, Cabezas M
    J Biol Chem, 2014-10-27;289(51):35421-30.
    Species: Hamster, Human
    Sample Types: Whole Cells
    Applications: Blocking, Flow Cytometry
  32. Hormonal modulators of glial ABCA1 and apoE levels.
    Authors: Fan, Jianjia, Shimizu, Yoko, Chan, Jeniffer, Wilkinson, Anna, Ito, Ayaka, Tontonoz, Peter, Dullaghan, Edie, Galea, Liisa A, Pfeifer, Tom, Wellington, Cheryl L
    J Lipid Res, 2013-09-02;54(11):3139-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013-02-19;288(15):10805-18.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  34. The proprotein convertase PC7: unique zymogen activation and trafficking pathways.
    Authors: Rousselet E, Benjannet S, Hamelin J, Canuel M, Seidah NG
    J. Biol. Chem., 2010-11-12;286(4):2728-38.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  35. Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
    Authors: Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG
    J. Biol. Chem., 2009-07-27;284(42):28856-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. Author Correction: Proteolysis of the low density lipoprotein receptor by bone morphogenetic protein-1 regulates cellular cholesterol uptake
    Authors: S Banerjee, RJ Andrew, CJ Duff, K Fisher, CD Jackson, CB Lawrence, N Maeda, DS Greenspan, KAB Kellett, NM Hooper
    Sci Rep, 2020-02-11;10(1):2681.
  37. Hypervariable Region 1 Deletion and Required Adaptive Envelope Mutations Confer Decreased Dependency on Scavenger Receptor Class B Type I and Low-Density Lipoprotein Receptor for Hepatitis C Virus
    Authors: Jannick Prentoe, Stéphanie B. N. Serre, Santseharay Ramirez, Alfredo Nicosia, Judith M. Gottwein, Jens Bukh
    Journal of Virology
  38. BLOS1 mediates kinesin switch during endosomal recycling of LDL receptor
    Authors: Chang Zhang, Chanjuan Hao, Guanghou Shui, Wei Li
    eLife
  39. Downregulation of low-density lipoprotein receptor mRNA in lymphatic endothelial cells impairs lymphatic function through changes in intracellular lipids
    Authors: Laurent Vachon, Ali Smaani, Nolwenn Tessier, Gabriel Jean, Annie Demers, Andreea Milasan et al.
    Theranostics

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Human LDLR Antibody
By Anonymous on 11/12/2020
Application: Flow Sample Tested: Peritoneal macrophages Species: Human
Human LDLR Antibody
By Anonymous on 10/13/2020
Application: B/N Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Blocking of LDL receptor in HEK293 cells by the Human LDLR Antibody (AF2148). HEK cells were incubated with Phrodo Red LDL at 10 μg/ml in the presence of 10 μg/ml of the blocking human LDLR antibody (AF2148).

Block/Neutralize Human LDLR Antibody AF2148
Human LDL R Antibody
By Anonymous on 04/21/2016
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human