Human LOX-1/OLR1 Antibody Summary
Ser61-Gln273
Accession # P78380
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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LOX‑1/OLR1 in Human Placenta. LOX-1/OLR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human LOX-1/OLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1798) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human LOX-1/OLR1 by Immunocytochemistry/Immunofluorescence Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human LOX-1/OLR1 by Western Blot Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human LOX-1/OLR1 by Western Blot Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human LOX-1/OLR1 by Knockdown Validated Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LOX-1/OLR1
Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2 - 4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5 - 7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The human LOX-1 gene encodes a 273 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (1, 6). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca2+-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells and dendritic cells (6 - 8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. Human LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5 - 9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.
- Sawamura, T. et al. (1997) Nature 386:73.
- Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223.
- Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
- Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
- Jono, T. et al. (2002) FEBS Lett. 511:170.
- Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
- Delneste, Y. et al. (2002) Immunity 17:353.
- Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
- Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
- Chen, J. et al. (2003) Circ. Res. 94:370.
Product Datasheets
Citations for Human LOX-1/OLR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Relationship of Soluble Lectin-Like Low-Density Lipoprotein Receptor-1 (sLOX-1) With Inflammation and Coronary Plaque Progression in Psoriasis
Authors: Florida, EM;Li, H;Hong, CG;Ongstad, EL;Gaddipati, R;Sitaula, S;Varma, V;Parel, PM;O'Hagan, R;Chen, MY;Teague, HL;Playford, MP;Karathanasis, SK;Collén, A;Mehta, NN;Remaley, AT;Sorokin, AV;
Journal of the American Heart Association
Species: Human
Sample Types: Serum
Applications: ELISA Detection -
LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-kappa B pathways under hypoxic-ischemic conditions
Authors: Yoshinori Aoki, Hongmei Dai, Fumika Furuta, Tomohisa Akamatsu, Takuya Oshima, Naoto Takahashi et al.
Cell Communication and Signaling
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LOX-1: A potential driver of cardiovascular risk in SLE patients
Authors: D Sagar, R Gaddipati, EL Ongstad, N Bhagroo, LL An, J Wang, M Belkhodja, S Rahman, Z Manna, MA Davis, S Hasni, R Siegel, M Sanjuan, J Grimsby, R Kolbeck, S Karathanas, GP Sims, R Gupta
PLoS ONE, 2020-03-17;15(3):e0229184.
Species: Human
Sample Types: Serum
Applications: ELISA Detection -
Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
Immunol. Cell Biol., 2018-02-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Lectin-like oxidized LDL receptor-1 is an enhancer of tumor angiogenesis in human prostate cancer cells.
Authors: Gonzalez-Chavarria I, Cerro R, Parra N, Sandoval F, Zuniga F, Omazabal V, Lamperti L, Jimenez S, Fernandez E, Gutierrez N, Rodriguez F, Onate S, Sanchez O, Vera J, Toledo J
PLoS ONE, 2014-08-29;9(8):e106219.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Overexpression of scavenger receptor LOX-1 in endothelial cells promotes atherogenesis in the ApoE(-/-) mouse model
Authors: Stephen J. White, Graciela B. Sala-Newby, Andrew C. Newby
Cardiovascular Pathology
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Acute atrial tachyarrhythmia induces angiotensin II type 1 receptor-mediated oxidative stress and microvascular flow abnormalities in the ventricles.
Authors: Goette A, Bukowska A, Dobrev D, Pfeiffenberger J, Morawietz H, Strugala D, Wiswedel I, Rohl FW, Wolke C, Bergmann S, Bramlage P, Ravens U, Lendeckel U
Eur. Heart J., 2009-03-05;30(11):1411-20.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: Western Blot -
OxLDL induces endothelial dysfunction and death via TRAF3IP2: inhibition by HDL3 and AMPK activators
Authors: Valente AJ, Irimpen AM, Siebenlist U et al.
Free Radic Biol Med
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