Human Lyp Antibody Summary
Ser306-Ser684
Accession # Q9Y2R2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Lyp by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell lines. PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lyp at approximately 108 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Lyp in Human PBMCs. Lyp was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human Lyp by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyp at approximately 116-118 kDa (as indicated) using 3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lyp
Lymphoid phosphatase (Lyp), also called LyPTP, Protein Tyrosine Phosphatase, Nonreceptor-type 22 (PTPN22), and PEST-domain Phosphatase (PEP), dephosphorylates tyrosine residues in proteins. Found primarily in bone marrow and lymphoid cell lines, an R620W gain-of-function mutation in Lyp has been associated with susceptibility to autoimmune diseases such as type I diabetes, rheumatoid arthritis, and lupus erythematosus. Mature PEP knockout mice have an excess of CD8 positive T-cells and an exaggerated antigen-induced proliferative response.
Product Datasheets
Citations for Human Lyp Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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PTPN22 Modulates Macrophage Polarization and Susceptibility to Dextran Sulfate Sodium–Induced Colitis
Authors: Hui-Hsin Chang, Shi-Chuen Miaw, William Tseng, Yi-Wei Sun, Chih-Chun Liu, Hsiao-Wei Tsao et al.
The Journal of Immunology
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Targeted Deep Sequencing in Multiple-Affected Sibships of European Ancestry Identifies Rare Deleterious Variants in PTPN22 That Confer Risk for Type 1 Diabetes.
Authors: Ge Y, Onengut-Gumuscu S, Quinlan AR et al.
Diabetes
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UBASH3A Interacts with PTPN22 to Regulate IL2 Expression and Risk for Type 1 Diabetes
Authors: Newman, JRB;Concannon, P;Ge, Y;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation -
PTPN22.6, a dominant negative isoform of PTPN22 and potential biomarker of rheumatoid arthritis.
Authors: Chang HH, Tai TS, Lu B
PLoS ONE, 2012-03-12;7(3):e33067.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Autoimmune-associated PTPN22 R620W variation reduces phosphorylation of lymphoid phosphatase on an inhibitory tyrosine residue.
Authors: Fiorillo E, Orru V, Stanford SM, Liu Y, Salek M, Rapini N, Schenone AD, Saccucci P, Delogu LG, Angelini F, Manca Bitti ML, Schmedt C, Chan AC, Acuto O, Bottini N
J. Biol. Chem., 2010-06-09;285(34):26506-18.
Species: Human, Primate - Cholrocebus pygerythrus (Vervet Monkey)
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Efficient CRISPR/Cas9 Disruption of Autoimmune-Associated Genes Reveals Key Signaling Programs in Primary Human T Cells
Authors: Warren Anderson, Jerill Thorpe, S. Alice Long, David J. Rawlings
The Journal of Immunology
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Lack of the Phosphatase PTPN22 Increases Adhesion of Murine Regulatory T Cells to Improve Their Immunosuppressive Function
Authors: Rebecca J. Brownlie, Lisa A. Miosge, Demetrios Vassilakos, Lena M. Svensson, Andrew Cope, Rose Zamoyska
Science Signaling
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A molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22
Authors: Hui-Hsin Chang, Guang-Yaw Liu, Nishant Dwivedi, Bo Sun, Yuko Okamoto, Jennifer D. Kinslow et al.
JCI Insight
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Altered expression of protein tyrosine phosphatase, non-receptor type 22 isoforms in systemic lupus erythematosus
Authors: Hui-Hsin Chang, William Tseng, Jing Cui, Karen Costenbader, I-Cheng Ho
Arthritis Research & Therapy
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Goat anti-PTPN22 from R&D used in flow cytometry, in combination with a secondary anti-goat conjugated to AF488.
Samples: PBMCs with non-targeting control and PBMCs with gRNA targeted to PTPN22/LYP. gRNAs from IDT.
Used to detect PTPN22/LYP in primary immune cells. Although a few bands can be observed per lane, the one running at 100 kDa corresponds to PTPN22/LYP (confirmed by siRNA).
