Human/Mouse NeuroD2 Antibody

Catalog #: AF2775 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2775
AF2775-SP

Detection of Mouse NeuroD2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NeuroD2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human NeuroD2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human NeuroD1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NeuroD2
Ser174-Asn382
Accession # Q15784

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse NeuroD2 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Human NeuroD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NeuroD2 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NeuroD2

NeuroD2 (Neurogenic differentiation factor 2; also NDRF and KW8) is a 41 kDa nuclear member of the neuroD family of transcription factors. It is expressed in developing and mature neurons such as hippocampal granular neurons, and acts (in part) to repress factors that would otherwise block multipotential cell commitment to a neuronal lineage. NeuroD2 is presumed to act as a heterodimer with other bHLH transcription factors. Human NeuroD2 is 382 amino acids (aa) in length. It contains a poly-Glu region (aa 82-91), an NLS (aa 107-113), a DNA-binding HLH domain (aa 119-178) and a poly-Gly segment (aa 282-285). Based on rat studies, human NeuroD2 will undergo variable phosphorylation in the C-terminal region. Over aa 174-382, human and mouse NeuroD2 possess identical amino acid sequences.

Long Name

Neurogenic Differentiation Factor 2

Entrez Gene IDs

4761 (Human)

Alternate Names

BHLHA1; bHLHa1neurogenic differentiation factor 2; Class A basic helix-loop-helix protein 1; MGC26304; NDRF; NDRFneurogenic basic-helix-loop-helix protein; NeuroD2; NeuroD-related factor; neurogenic differentiation 2