Home / Contactin-2/TAG1 / Human/Mouse/Rat Contactin-2/TAG1 Antibody

Human/Mouse/Rat Contactin-2/TAG1 Antibody

Catalog #: AF1714
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1714
AF1714-SP
Detection of Human, Mouse, and Rat Contactin‑2/TAG1 by Western Blot.
3 Images
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Contactin-2/TAG1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Contactin‑2/TAG1 in direct ELISAs. Detects human, mouse, and rat Contactin‑2/TAG1 in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Contactin-3, rhContactin-4, rhContactin-5 and rhContactin-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Contactin‑2/TAG1
Leu29-Asn1012
Accession # Q02246
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
Human brain (cerebellum and hippocampus)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Contactin-2/TAG1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Contactin‑2/TAG1 by Western Blot. Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin-2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Contactin-2/TAG1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Contactin‑2/TAG1 in Human Brain. Contactin-2/TAG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human Contactin‑2/TAG1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum and hippocampus), loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 160 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl‑phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1‑3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III‑like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3‑5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3‑6). In mature myelinated fibers, Contactin‑2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin‑associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7‑10). The axonal Contactin‑2 interacts in cis with caspr2, and in trans with another Contactin‑2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

References
  1. Wolfer, D. & R. J. Giger (1994) Swissprot Accession # Q61330.
  2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
  3. Karagogeos, D. (2003) Front. Biosci.8:s1304.
  4. Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
  5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
  6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
  7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
  8. Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
  9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Entrez Gene IDs
6900 (Human); 21367 (Mouse)
Alternate Names
Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat Contactin-2/TAG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Nkx2-5 Loss of Function in the His-Purkinje System Hampers Its Maturation and Leads to Mechanical Dysfunction
    Authors: Choquet, C;Sicard, P;Vahdat, J;Nguyen, THM;Kober, F;Varlet, I;Bernard, M;Richard, S;Kelly, RG;Lalev�e, N;Miquerol, L;
    Journal of cardiovascular development and disease
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. p75NTR prevents the onset of cerebellar granule cell migration via RhoA activation
    Authors: Juan P Zanin, Wilma J Friedman
    eLife
  3. The m6 A Readers YTHDF1 and YTHDF2 Synergistically Control Cerebellar Parallel Fiber Growth by Regulating Local Translation of the Key Wnt5a Signaling Components in Axons
    Authors: Jun Yu, Yuanchu She, Lixin Yang, Mengru Zhuang, Peng Han, Jianhui Liu et al.
    Advanced Science
  4. Nkx2-5 defines distinct scaffold and recruitment phases during formation of the murine cardiac Purkinje fiber network
    Authors: C Choquet, RG Kelly, L Miquerol
    Nat Commun, 2020-10-20;11(1):5300.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Dorsal-to-Ventral Cortical Expansion Is Physically Primed by Ventral Streaming of Early Embryonic Preplate Neurons
    Authors: K Saito, M Okamoto, Y Watanabe, N Noguchi, A Nagasaka, Y Nishina, T Shinoda, A Sakakibara, T Miyata
    Cell Rep, 2019-11-05;29(6):1555-1567.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Genetic analysis of the organization, development and plasticity of corneal innervation in mice
    Authors: N Bouheraoua, S Fouquet, M Teresa Mar, D Karagogeos, L Laroche, A Chédotal
    J. Neurosci., 2018-12-26;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Embryo
    Applications: IHC
  7. Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy
    Authors: C Choquet, THM Nguyen, P Sicard, E Buttigieg, TT Tran, F Kober, I Varlet, R Sturny, MW Costa, RP Harvey, C Nguyen, P Rihet, S Richard, M Bernard, RG Kelly, N Lalevée, L Miquerol
    PLoS Genet., 2018-07-06;14(7):e1007502.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate
    Authors: MZ Ozair, C Kirst, BL van den Be, A Ruzo, T Rito, AH Brivanlou
    Cell Stem Cell, 2018-06-21;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Segregation of Central Ventricular Conduction System Lineages in Early SMA+ Cardiomyocytes Occurs Prior to Heart Tube Formation
    Authors: Caroline Choquet, Laetitia Marcadet, Sabrina Beyer, Robert G. Kelly, Lucile Miquerol
    Journal of Cardiovascular Development and Disease
  10. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing
    Authors: Berenice Hernandez-Enriquez, Zhuhao Wu, Edward Martinez, Olav Olsen, Zaven Kaprielian, Patricia F. Maness et al.
    Genes & Development
  11. Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.
    Authors: Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R
    J. Neurosci., 2009-05-20;29(20):6677-90.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: ICC, Immunoprecipitation, Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Contactin-2/TAG1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Contactin-2/TAG1 Antibody and earn rewards!

Have you used Human/Mouse/Rat Contactin-2/TAG1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review