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Human/Mouse/Rat FKBP51 Antibody

Catalog #: AF4094
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4094
AF4094-SP
FKBP51 in Human Prostate.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FKBP51 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat FKBP51. In Western blots, less than 1% cross-reactivity with recombinant human FKBP12, FKBP13, FKBP38, and FKBP52 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FKBP51
Thr2-Val457
Accession # Q13451
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.3 µg/mL
See below
Simple Western
3 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
FKBP51 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in FKBP51 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FKBP51 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

FKBP51 in Human Prostate. FKBP51 was detected in immersion fixed paraffin-embedded sections of human prostate using 15 µg/mL Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human/Mouse/Rat FKBP51 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat FKBP51 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human FKBP51 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human FKBP51 by Simple WesternTM. Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FKBP51 at approximately 61 kDa (as indicated) using 3 µg/mL of Goat Anti-Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human FKBP51 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human FKBP51 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FKBP51 knockout HeLa cell line (KO). PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FKBP51 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP51

FK506 binding protein, 51 kDa molecular weight (FKBP51) is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans- proline residues critical for proper folding of proteins. The macrolide immunosuppressants FK506 and Rapamycin are FKBP51 inhibitors. FKBP51 levels are induced by glucocorticoids. It associates with HSP90 complexes that are critical for the proper folding of steroid receptors. Single nucleotide polymorphisms in FKBP51 have been associated with major depression and hyper-responsiveness to antidepressants.

Long Name
51 kDa FK506 Binding Protein
Entrez Gene IDs
2289 (Human); 14229 (Mouse); 361810 (Rat)
Alternate Names
51 kDa FKBP; 54 kDa progesterone receptor-associated immunophilin; AIG6,51 kDa FK506-binding protein; Androgen-regulated protein 6; Dit1; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP5; FKBP-5; FKBP51; FKBP-51; FKBP51PPIase FKBP5; FKBP54; FKBP54EC 5.2.1.8; HSP90-binding immunophilin; MGC111006; p54; P54,51 kDa FK506-binding protein 5; peptidyl-prolyl cis-trans isomerase FKBP5; peptidylprolyl cis-trans isomerase; PPIase; Ptg-10; rotamase; T-cell FK506-binding protein

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Citations for Human/Mouse/Rat FKBP51 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action.
    Authors: Tannour-Louet M, Han S, Louet JF et al.
    Nat Med
  2. Decidual cell FKBP51-progesterone receptor binding mediates maternal stress-induced preterm birth
    Authors: O Guzeloglu-, N Semerci, X Guo, K Larsen, A Ozmen, S Arlier, D Mutluay, C Nwabuobi, B Sipe, I Buhimschi, C Buhimschi, F Schatz, UA Kayisli, CJ Lockwood
    Proceedings of the National Academy of Sciences of the United States of America, 2021-03-16;118(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: In Situ PLA
  3. PlexinB1 Promotes Nuclear Translocation of the Glucocorticoid Receptor.
    Authors: Williamson M, Garg R, Wells C et al.
    Cells.
  4. Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice
    Authors: L Janeckova, K Kostovciko, J Svec, M Stastna, H Strnad, M Kolar, T Hudcovic, J Stancikova, J Tureckova, N Baloghova, E Sloncova, K Galuskova, H Tlaskalova, V Korinek
    Int J Mol Sci, 2019-03-29;20(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Glucocorticoid-regulated genes in eosinophilic esophagitis: a role for FKBP51.
    Authors: Caldwell JM, Blanchard C, Collins MH, Putnam PE, Kaul A, Aceves SS, Bouska CA, Rothenberg ME
    J. Allergy Clin. Immunol., 2010-04-01;125(4):879-888.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  6. Annotating STEAP1 Regulation in Prostate Cancer with 89Zr Immuno-PET
    Authors: Michael G. Doran, Philip A. Watson, Sarah M. Cheal, Daniel E. Spratt, John Wongvipat, Jeffrey M. Steckler et al.
    Journal of Nuclear Medicine
  7. PlexinB1 Promotes Nuclear Translocation of the Glucocorticoid Receptor.
    Authors: Williamson M, Garg R, Wells C et al.
    Cells.
  8. Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding
    Authors: Ozlem Guzeloglu Kayisli, Umit A. Kayisli, Murat Basar, Nihan Semerci, Frederick Schatz, Charles J. Lockwood
    PLOS ONE
  9. Androgens modulate glucocorticoid receptor activity in adipose tissue and liver
    Authors: Dieuwertje C E Spaanderman, Mark Nixon, Jacobus C Buurstede, Hetty H C M Sips, Maaike Schilperoort, Eline N Kuipers et al.
    Journal of Endocrinology

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