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Human/Mouse/Rat Neuropilin-2 Antibody

Catalog #: AF2215
32 Citations 2 Reviews Datasheet / COA / SDS
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AF2215
AF2215-SP
Detection of Human Neuropilin‑2 by Western Blot.
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Citations (32)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Neuropilin-2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Neuropilin-2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑2
Gln23-Tyr855
Accession # Q7LBX6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
HUVEC human umbilical vein endothelial cells
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-10 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human VEGF165  (Catalog # 293-VE) to immobilized Recombinant Human Neuropilin-2 Fc Chimera  (Catalog # 2215-N2) coated at 1 µg/mL (100 µL/well). At 20 µg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Neuropilin-2 antibody by Western Blot. View Larger

Detection of Human Neuropilin‑2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF109). A specific band was detected for Neuropilin-2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neuropilin-2 antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Neuropilin‑2 in Human Pancreatic Cancer Tissue. Neuropilin-2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2215) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human, Mouse, and Rat Neuropilin-2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human, Mouse, and Rat Neuropilin‑2 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin-2 at approximately 140 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2215) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Flow Cytometry View Larger

Detection of Neuropillin-2 in HUVEC Human Cell Line by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human/Mouse/Rat Neuropillin-2 Polyclonal Antibody (Catalog # AF2215, filled histogram) or Goat IgG control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated anti-Goat IgG (F0107). Staining was performed using our Staining Surface Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-2

Neuropilin-2 (Npn-2) is a 120 kDa, type I transmembrane (TM) glycoprotein that is related to the semaphorin receptor now known as Neuropilin-1 (1). Npn-2 is a complex molecule with multiple splice forms. Five transmembrane forms are known, and one 62 kDa soluble form has been identified (2). Based on the originally reported precursor size of 909 amino acids (aa), the “standard” precursor in human will have a 20 aa signal sequence, an 842 aa extracellular region, a 25 aa TM segment, and a 42 aa cytoplasmic tail (1). The extracellular region contains two N-terminal CUB (C1r/Ugef/BMP-1) domains, two jellyroll-shaped coagulation factor V type C domains, and a juxtamembrane MAM (meprin/A-5 protein/tyrosine phosphatase μ) domain (1, 3). The CUB and factor V domain are involved in VEGF and semaphorin binding. The MAM domain appears necessary for signaling through plexin-1 (4). The five transmembrane isoforms all share the same CUB, factor V and MAM domains. Splicing begins at aa 809, seven amino acids after the end of the MAM domain, and it involves the end of the extracellular region, the TM segment, and the cytoplasmic domain (a total of 101 aa). Two of the four variants show a complete replacement of these 101 aa with a totally unrelated stretch of approximately 90 aa. This creates a new TM and cytoplasmic tail. These forms are called “Npn-2b” forms. Two other isoforms (plus the standard 909 aa form) retain the 101 aa stretch, and add either 17 or 22 aa to the end of the extracellular region. These forms are called “Npn-2a” forms. The isoform offered by R&D Systems is the “a” form with the 17 aa addition. This isoform shows 94% aa identity to the equivalent regions in mouse and rat Npn-2. The soluble form of Npn-2 is 555 aa in precursor length, and contains the two CUB domains plus the first 1½ factor V type C domains (1). Npn-2 binds Sema3B through F, and VEGF isoforms 165, 145, PlGF-2, and VEGF-C (5). It is known to form homodimers and heterodimers with Npn-1, and it forms receptor complexes with plexin-1 and VEGF R1 (4, 5). Npn-2 is found on a variety of cell types including neurons (motor, autonomic, sensory), vascular endothelial cells, Schwann cells and pancreatic acinar cells.

References
  1. Chen, H. et al. (1997) Neuron 19:547.
  2. Rossignol, M. et al. (2000) Genomics 70:211.
  3. He, Z. and M. Tessier-lavigne (1997) Cell 90:739.
  4. Nakamura, F. and Y. Goshima (2002) Adv. Exp. Med. Biol. 515:55.
  5. Neufeld, G. et al. (2002) Adv. Exp. Med. Biol. 515:81.
Entrez Gene IDs
8828 (Human); 18187 (Mouse); 81527 (Rat)
Alternate Names
MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Human/Mouse/Rat Neuropilin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Neuropilin-2b facilitates resistance to tyrosine kinase inhibitors in non–small cell lung cancer
    Authors: Anastasios Dimou, Cecile Nasarre, Yuri K. Peterson, Rose Pagano, Monika Gooz, Patrick Nasarre et al.
    The Journal of Thoracic and Cardiovascular Surgery
  2. Neuropilin-2 promotes branching morphogenesis in the mouse mammary gland
    Authors: Hira Lal Goel, Donggoo Bae, Bryan Pursell, Lindsey M. Gouvin, Shaolei Lu, Arthur M. Mercurio
    Development
  3. Influence of VEGF-A, VEGFR-1-3, and neuropilin 1-2 on progression-free: and overall survival in WHO grade II and III meningioma patients
    Authors: Simon Bernatz, Daniel Monden, Florian Gessler, Tijana Radic, Elke Hattingen, Christian Senft et al.
    Journal of Molecular Histology
  4. Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination
    Authors: Hung, YH;Hou, YC;Hsu, SH;Wang, LY;Tsai, YL;Shan, YS;Su, YY;Hung, WC;Chen, LT;
    American journal of cancer research
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Pyramidal neuron subtype diversity governs microglia states in the neocortex
    Authors: JA Stogsdill, K Kim, L Binan, SL Farhi, JZ Levin, P Arlotta
    Nature, 2022-08-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance
    Authors: R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta
    Oncogene, 2022-07-19;40(3):111097.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Genetically modified macrophages accelerate myelin repair
    Authors: MS Aigrot, C Barthelemy, S Moyon, G Dufayet-Ch, L Izagirre-U, B Gillet-Leg, S Tada, L Bayón-Cord, JC Chara, C Matute, N Cartier, C Lubetzki, V Tepav?evi?
    Embo Molecular Medicine, 2022-07-13;0(0):e14759.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Neuropilin-2 regulates androgen-receptor transcriptional activity in advanced prostate cancer
    Authors: S Dutta, NS Polavaram, R Islam, S Bhattachar, S Bodas, T Mayr, S Roy, SAY Albala, MI Toma, A Darehshour, A Borkowetz, S Conrad, S Fuessel, M Wirth, GB Baretton, LC Hofbauer, P Ghosh, KJ Pienta, DL Klinkebiel, SK Batra, MH Muders, K Datta
    Oncogene, 2022-06-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Structural basis for HCMV Pentamer receptor recognition and antibody neutralization
    Authors: M Kschonsak, MC Johnson, R Schelling, EM Green, L Rougé, H Ho, N Patel, C Kilic, E Kraft, CP Arthur, AL Rohou, L Comps-Agra, N Martinez-M, L Perez, J Payandeh, C Ciferri
    Science Advances, 2022-03-11;8(10):eabm2536.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Prolactin-sensitive olfactory sensory neurons regulate male preference in female mice by modulating responses to chemosensory cues
    Authors: M Aoki, I Gamayun, A Wyatt, R Grünewald, M Simon-Thom, SE Philipp, O Hummel, S Wagenpfeil, K Kattler, G Gasparoni, J Walter, S Qiao, DR Grattan, U Boehm
    Science Advances, 2021-10-08;7(41):eabg4074.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. The Role of Heparan Sulfate and Neuropilin 2 in VEGFA Signaling in Human Endothelial Tip Cells and Non-Tip Cells during Angiogenesis In Vitro
    Authors: MG Dallinga, YI Habani, AWM Schimmel, GM Dallinga-T, CJF van Noorde, I Klaassen, RO Schlingema
    Cells, 2021-04-16;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  12. PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination
    Authors: G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille
    Cell Reports, 2021-01-26;34(4):108644.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. MiR-146a Regulates Migration and Invasion by Targeting NRP2 in Circulating-Tumor Cell Mimicking Suspension Cells
    Authors: Y Do, JG Cho, JY Park, S Oh, D Park, KH Yoo, MS Lee, BS Kwon, J Kim, Y Yang
    Genes, 2020-12-30;12(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Atypical cadherin Fat4 orchestrates lymphatic endothelial cell polarity in response to flow
    Authors: KL Betterman, DL Sutton, GA Secker, J Kazenwadel, A Oszmiana, L Lim, N Miura, L Sorokin, BM Hogan, ML Kahn, H McNeill, NL Harvey
    J. Clin. Invest., 2020-06-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Characterization of the human GnRH neuron developmental transcriptome using a GNRH1-TdTomato reporter line in human pluripotent stem cells
    Authors: C Lund, V Yellapraga, S Vuoristo, D Balboa, S Trova, C Allet, N Eskici, K Pulli, P Giacobini, T Tuuri, T Raivio
    Dis Model Mech, 2020-03-13;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Defective AMH signaling disrupts GnRH neuron development and function and contributes to hypogonadotropic hypogonadism
    Authors: SA Malone, GE Papadakis, A Messina, NEH Mimouni, S Trova, M Imbernon, C Allet, I Cimino, J Acierno, D Cassatella, C Xu, R Quinton, G Szinnai, P Pigny, L Alonso-Cot, L Masgrau, JD Maréchal, V Prevot, N Pitteloud, P Giacobini
    Elife, 2019-07-10;8(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
    Authors: Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C
    Cell, 2018-07-26;174(5):1158-1171.e19.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  19. NRP2 and CD63 Are Host Factors for Lujo Virus Cell Entry
    Authors: M Raaben, LT Jae, AS Herbert, AI Kuehne, SH Stubbs, YY Chou, VA Blomen, T Kirchhause, JM Dye, TR Brummelkam, SP Whelan
    Cell Host Microbe, 2017-11-08;22(5):688-696.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  20. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017-06-09;12(6):e0179257.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Promotion of metastasis of thyroid cancer cells via NRP-2-mediated induction
    Oncol Lett, 2016-09-20;12(5):4224-4230.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Class 3 semaphorins induce F-actin reorganization in human dendritic cells: Role in cell migration
    J Leukoc Biol, 2016-07-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC-Fr, Neutralization
  23. Neuropilin-2 Regulates Endosome Maturation and EGFR Trafficking to Support Cancer Cell Pathobiology.
    Authors: Dutta S, Roy S, Polavaram N, Stanton M, Zhang H, Bhola T, Honscheid P, Donohue T, Band H, Batra S, Muders M, Datta K
    Cancer Res, 2015-11-11;76(2):418-28.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells.
    Authors: Wang, Hong-Bo, Zhang, Hua, Zhang, Jing-Pin, Li, Yan, Zhao, Bo, Feng, Guo-Kai, Du, Yong, Xiong, Dan, Zhong, Qian, Liu, Wan-Li, Du, Huamao, Li, Man-Zhi, Huang, Wen-Lin, Tsao, Sai Wah, Hutt-Fletcher, Lindsey, Zeng, Yi-Xin, Kieff, Elliott, Zeng, Mu-Sheng
    Nat Commun, 2015-02-11;6(0):6240.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  25. Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.
    Authors: Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M
    Oncoimmunology, 2014-06-25;3(0):e29080.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
    Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G
    PLoS ONE, 2014-01-02;9(1):e84179.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Neuropilin-2 Is upregulated in lung cancer cells during TGF-beta1-induced epithelial-mesenchymal transition.
    Authors: Nasarre P, Gemmill R, Potiron V, Roche J, Lu X, Baron A, Korch C, Garrett-Mayer E, Lagana A, Howe P, Drabkin H
    Cancer Res, 2013-10-11;73(23):7111-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
    Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
    EMBO J, 2013-01-08;32(5):629-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  29. Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
    Authors: Takeuchi H, Inokuchi K, Aoki M
    Cell, 2010-06-11;141(6):1056-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis.
    Authors: Fassold A, Falk W, Anders S, Hirsch T, Mirsky VM, Straub RH
    Arthritis Rheum., 2009-10-01;60(10):2892-901.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  31. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity
    Authors: Luis F. Martins, Ilaria Brambilla, Alessia Motta, Stefano de Pretis, Ganesh Parameshwar Bhat, Aurora Badaloni et al.
    Neuron
  32. Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma
    Authors: Aurore Dumond, Etienne Brachet, Jérôme Durivault, Valérie Vial, Anna K. Puszko, Yves Lepelletier et al.
    Journal of Experimental & Clinical Cancer Research

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Human/Mouse/Rat Neuropilin‑2 Antibody
By Zhipeng Meng on 04/21/2017
Human/Mouse/Rat Neuropilin‑2 Antibody AF2215
Human/Mouse/Rat Neuropilin-2 Antibody
By Anonymous on 09/01/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Neonatal dorsal root ganglia cells Species: Mouse