Home / Troponin T / Human/Mouse/Rat Troponin T (Cardiac) Antibody

Human/Mouse/Rat Troponin T (Cardiac) Antibody

Catalog #: MAB18742
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB18742-100
MAB18742-SP
Detection of Human, Mouse, and Rat Troponin T by Western Blot.
3 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Troponin T (Cardiac) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Troponin T in ELISA. Detects human, mouse, and rat Troponin T in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 990033
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Troponin T (Cardiac) antigen
Accession # P45379
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody to detect human Troponin T (Cardiac) when paired with Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18741).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Immunohistochemistry
0.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Troponin T antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Troponin T by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Troponin T at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Troponin T antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Troponin T in Human Heart. Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

ELISA Human Troponin T (Cardiac) Antibody in ELISA Standard Curve. View Larger

Human Troponin T (Cardiac) ELISA Standard Curve. Recombinant Human Troponin T protein was serially diluted 2-fold and captured by Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin T

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Entrez Gene IDs
7139 (Human)
Alternate Names
Cardiac Muscle Troponin T; Cardiomyopathy, Dilated 1D (Autosomal Dominant); Cardiomyopathy, Hypertrophic; CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TNNT2; TnTC; troponin T type 2 (cardiac); Troponin T; troponin T, cardiac muscle; Troponin T2, Cardiac Type; Truncated Cardiac Troponin T

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat Troponin T (Cardiac) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Hematopoietic Prostaglandin D Synthase Is Increased in Mast Cells and Pericytes in Autopsy Myocardial Specimens from Patients with Duchenne Muscular Dystrophy
    Authors: Hamamura, K;Yoshida, Y;Oyama, K;Li, J;Kawano, S;Inoue, K;Toyooka, K;Yamadera, M;Matsunaga, N;Matsumura, T;Aritake, K;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. m6A demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3
    Authors: Gao R, Yang K, Qu Y et al.
    iScience

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Troponin T (Cardiac) Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Troponin T (Cardiac) Antibody and earn rewards!

Have you used Human/Mouse/Rat Troponin T (Cardiac) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review