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Human/Mouse WDR5 Antibody

Catalog #: AF5810
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5810
AF5810-SP
Detection of Human WDR5 by Western Blot.
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Citations (7)
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Human/Mouse WDR5 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse WDR5 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human WDR5
Met1-Lys120
Accession # P61964
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human WDR5 antibody by Western Blot. View Larger

Detection of Human WDR5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for WDR5 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry WDR5 antibody in Human B-Cell Lymphoma by Immunohistochemistry (IHC-P). View Larger

WDR5 in Human B-Cell Lymphoma. WDR5 was detected in immersion fixed paraffin-embedded sections of human B-cell lymphoma using Goat Anti-Human/Mouse WDR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5810) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/ Immunofluorescence Detection of Human WDR5 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human WDR5 by Immunocytochemistry/Immunofluorescence SRY cooperates with WDR5 to induce Sox9 expression.(A) Immunofluorescence analysis of HA-tagged SRY and WDR5 in LNCaP cells. (B) ChIP-reChIP (anti-HA antibody ChIP followed by anti-WDR5 antibody ChIP) analysis of HA-tagged SRY and WDR5 on Sox9 promoter, or Sox9 promoter proximal upstream region. Graphs show mean ± SD, n = 3. (C) Coimmunoprecipitation of WDR5 and HA-tagged SRY from LNCaP cells. (D) Quantitative real time PCR analysis of Sox9 levels from LNCaP cells containing SF1, SF1+WDR5, SF1+SRY, or SF1+SRY+WDR5. Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (E) Quantitative real time PCR analysis of beta -catenin levels as in (D). Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (F) Western blot analysis of cellular lysate from cells as in (D). (G) Western blot analyses of cellular extracts from WDR5i-1 and WDR5i-2 or scrambled control (Ctrl) LNCaP cells with indicated antibodies. (H) WDR5 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean ± SD, n = 3. *P<0.05 compared to the scrambled control, Student's t-test. (I) Sox9 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean±SD, n = 3. **P<0.01 compared to the scrambled control, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0034327), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human WDR5 by Western Blot View Larger

Detection of Human WDR5 by Western Blot SRY cooperates with WDR5 to induce Sox9 expression.(A) Immunofluorescence analysis of HA-tagged SRY and WDR5 in LNCaP cells. (B) ChIP-reChIP (anti-HA antibody ChIP followed by anti-WDR5 antibody ChIP) analysis of HA-tagged SRY and WDR5 on Sox9 promoter, or Sox9 promoter proximal upstream region. Graphs show mean ± SD, n = 3. (C) Coimmunoprecipitation of WDR5 and HA-tagged SRY from LNCaP cells. (D) Quantitative real time PCR analysis of Sox9 levels from LNCaP cells containing SF1, SF1+WDR5, SF1+SRY, or SF1+SRY+WDR5. Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (E) Quantitative real time PCR analysis of beta -catenin levels as in (D). Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (F) Western blot analysis of cellular lysate from cells as in (D). (G) Western blot analyses of cellular extracts from WDR5i-1 and WDR5i-2 or scrambled control (Ctrl) LNCaP cells with indicated antibodies. (H) WDR5 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean ± SD, n = 3. *P<0.05 compared to the scrambled control, Student's t-test. (I) Sox9 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean±SD, n = 3. **P<0.01 compared to the scrambled control, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0034327), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human WDR5 by Western Blot View Larger

Detection of Human WDR5 by Western Blot SRY cooperates with WDR5 to induce Sox9 expression.(A) Immunofluorescence analysis of HA-tagged SRY and WDR5 in LNCaP cells. (B) ChIP-reChIP (anti-HA antibody ChIP followed by anti-WDR5 antibody ChIP) analysis of HA-tagged SRY and WDR5 on Sox9 promoter, or Sox9 promoter proximal upstream region. Graphs show mean ± SD, n = 3. (C) Coimmunoprecipitation of WDR5 and HA-tagged SRY from LNCaP cells. (D) Quantitative real time PCR analysis of Sox9 levels from LNCaP cells containing SF1, SF1+WDR5, SF1+SRY, or SF1+SRY+WDR5. Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (E) Quantitative real time PCR analysis of beta -catenin levels as in (D). Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (F) Western blot analysis of cellular lysate from cells as in (D). (G) Western blot analyses of cellular extracts from WDR5i-1 and WDR5i-2 or scrambled control (Ctrl) LNCaP cells with indicated antibodies. (H) WDR5 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean ± SD, n = 3. *P<0.05 compared to the scrambled control, Student's t-test. (I) Sox9 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean±SD, n = 3. **P<0.01 compared to the scrambled control, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0034327), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human WDR5 by Western Blot View Larger

Detection of Human WDR5 by Western Blot SRY cooperates with WDR5 to induce Sox9 expression.(A) Immunofluorescence analysis of HA-tagged SRY and WDR5 in LNCaP cells. (B) ChIP-reChIP (anti-HA antibody ChIP followed by anti-WDR5 antibody ChIP) analysis of HA-tagged SRY and WDR5 on Sox9 promoter, or Sox9 promoter proximal upstream region. Graphs show mean ± SD, n = 3. (C) Coimmunoprecipitation of WDR5 and HA-tagged SRY from LNCaP cells. (D) Quantitative real time PCR analysis of Sox9 levels from LNCaP cells containing SF1, SF1+WDR5, SF1+SRY, or SF1+SRY+WDR5. Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (E) Quantitative real time PCR analysis of beta -catenin levels as in (D). Graphs show mean ± SD, n = 3. *P<0.05, **P<0.01 compared to SF1 control; Student's t-test. (F) Western blot analysis of cellular lysate from cells as in (D). (G) Western blot analyses of cellular extracts from WDR5i-1 and WDR5i-2 or scrambled control (Ctrl) LNCaP cells with indicated antibodies. (H) WDR5 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean ± SD, n = 3. *P<0.05 compared to the scrambled control, Student's t-test. (I) Sox9 gene expression analysis by Q-RT-PCR of RNA from WDR5i-1, WDR5i-2 and scrambled control (Ctrl) LNCaP cells. Graphs show mean±SD, n = 3. **P<0.01 compared to the scrambled control, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0034327), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WDR5

WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a nuclear, 36‑40 kDa monomeric member of the WD family of repeat proteins. It is known to be expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is 334 amino acids (aa) in length. It contains seven WD domains (aa 43‑333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence.

Long Name
WD Repeat-containing Protein 5
Entrez Gene IDs
11091 (Human); 140858 (Mouse); 362093 (Rat)
Alternate Names
BIG3; BIG-3; BMP2-induced 3-kb gene protein; SWD3; SWD3, Set1c WD40 repeat protein, homolog; WD repeat domain 5; WD repeat-containing protein 5; WDR5; WD-repeat protein 5

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Citations for Human/Mouse WDR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. WDR5 is a prognostic biomarker of brain metastasis from non-small cell lung cancer
    Authors: Zheng Li, Nan Liang, Na Wang, Yan Jia, Cui Tian
    Frontiers in Oncology
  2. The Ash2l SDI Domain Is Required to Maintain the Stability and Binding of DPY30
    Authors: Mengjie Ma, Jiafeng Zhou, Zhihua Ma, Hanxue Chen, Liang Li, Lin Hou et al.
    Cells
  3. p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells
    Authors: Q Li, F Mao, B Zhou, Y Huang, Z Zou, AD denDekker, J Xu, S Hou, J Liu, Y Dou, RC Rao
    Cell Rep, 2020-01-14;30(2):465-480.e6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Expression of WD Repeat Domain 5 (WDR5) is Associated with Progression and Reduced Prognosis in Papillary Thyroid Carcinoma
    Authors: W Xu, L Wang, Y An, J Ye
    Med. Sci. Monit., 2019-05-20;25(0):3762-3770.
    Species: Human
    Sample Types: Paraffin-Embedded Tissue
    Applications: IHC
  5. PI3K/AKT-mediated upregulation of WDR5 promotes colorectal cancer metastasis by directly targeting ZNF407
    Authors: X Tan, S Chen, J Wu, J Lin, C Pan, X Ying, Z Pan, L Qiu, R Liu, R Geng, W Huang
    Cell Death Dis, 2017-03-16;8(3):e2686.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: ChIP, IHC, Western Blot
  6. GRHL3/GET1 and trithorax group members collaborate to activate the epidermal progenitor differentiation program.
    Authors: Hopkin, Amelia S, Gordon, William, Klein, Rachel H, Espitia, Francisc, Daily, Kenneth, Zeller, Michael, Baldi, Pierre, Andersen, Bogi
    PLoS Genet, 2012-07-19;8(7):e1002829.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis.
    Authors: Liu L, Yin Y, Li Y
    Cell Res., 2012-03-27;22(8):1258-69.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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