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Human Otx2 Antibody

Catalog #: AF1979
173 Citations 7 Reviews Datasheet / COA / SDS
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AF1979
AF1979-SP
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Detection of Human Otx2 by Western Blot.
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Citations (173)
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Reviews (7)
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Human Otx2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Otx2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Otx2 antibody by Western Blot. View Larger

Detection of Human Otx2 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Otx2 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Otx2 in NTera‑2 Human Cell Line. Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Otx2 antibody in Mouse Embryo by Immunohistochemistry (IHC-P). View Larger

Otx2 in Mouse Embryo. Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human Otx2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Otx2 by Simple WesternTM. Simple Western lane view shows lysates of IMR-32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence Detection of Human Otx2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Otx2 by Immunocytochemistry/Immunofluorescence Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). (A) Dermal fibroblasts derived from patient with SLE were reprogrammed into iPSCs using Sendai virus vectors and three clones (#1, #2 and #3) were characterized. RT‐PCR confirms the loss of transgenes in hiPSCs‐SLE (lanes 1, 2 and 3), presence (lane 4) in infected fibroblasts (ipF‐SLE) and absence of Sendai viral transgenes in parental fibroblasts (pF‐SLE) (lane 5). Full‐length gels are presented in File S2. (B) The hiPSCs‐SLE colonies expressed alkaline phosphatase. Scale bar, 500 μm. (C) RT‐qPCR analysis of pluripotency genes OCT4, NANOG, SOX2 and REX1 was performed in fibroblasts and in hiPSCs derived from patient with SLE. All expression values are normalized to GAPDH and relative donor fibroblasts. Data are mean ± SEM and all statistical analysis was made between hiPSCs‐SLE clones and relative fibroblasts by Student's t test showing P‐values ≤ .05 in each comparison. (D) PluriTest assays combines novelty score (blue) on x‐axis and pluripotency score (red) on y‐axis. hiPSCs‐SLE localize in the red cloud suggesting the empirical distribution of pluripotent cells compared to non‐pluripotent blue cloud. (E) Representative images of M‐FISH staining show normal karyotypes of hiPSCs‐SLE clones. (F) Immunofluorescence analysis of pluripotent stem cell markers Nanog (green), Oct4 (red) and co‐staining with DAPI (blue) in hiPSCs‐SLE. Scale bar, 50 μm. (G) Representative images of floating and adherent EBs derived from hiPSCs‐SLE at differentiation day 8 and 18, respectively. Scale bar, 500 μm. (H) RT‐qPCR results confirm the capability of hiPSCs‐SLE to differentiate into all three germ layers. The expression levels of GATA4, HAND1 and PAX6 in EBs are relative to undifferentiated hiPSCs. All expression values are normalized to GAPDH and relative hiPSCs. Data are mean ± SEM and all statistical analysis was made between EBs‐SLE and relative hiPSCs‐SLE clones by Student's t test showing P‐values ≤ .05 in each comparison. (I) Immunostaining of Otx2 (ectoderm marker), Sox17 (endoderm marker), Brachyury (mesoderm marker) and co‐staining with DAPI (blue) in EBs derived from hiPSCs‐SLE. Scale bar, 50 μm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31536674), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otx2

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References
  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.
Long Name
Orthodenticle Homolog 2
Entrez Gene IDs
5015 (Human)
Alternate Names
homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2

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Citations for Human Otx2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

173 Citations: Showing 1 - 10
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  1. Spatial Stem Cell Fate Engineering via Facile Morphogen Localization
    Authors: Jin G, Floy ME, Simmons AD et al.
    Advanced healthcare materials
  2. Transcriptional synergy as an emergent property defining cell subpopulation identity enables population shift
    Authors: S Okawa, C Saltó, S Ravichandr, S Yang, EM Toledo, E Arenas, A Del Sol
    Nat Commun, 2018-07-03;9(1):2595.
  3. Optic vesicle morphogenesis requires primary cilia
    Authors: Luciano Fiore, Nozomu Takata, Sandra Acosta, Wanshu Ma, Tanushree Pandit, Michael Oxendine et al.
    Developmental Biology
  4. Single-Cell Analysis of Human Retina Identifies Evolutionarily Conserved and Species-Specific Mechanisms Controlling Development
    Authors: Lu Y, Shiau F, Yi W et al.
    Dev. Cell
  5. Generation of an induced pluripotent stem cell (iPSC) line (EXSURGi001-A) from a patient homozygous for the p.Ala165Thr mutation in the MTARC1 gene
    Authors: Tang, P;Keshi, E;Wilken, S;Wutsdorff, L;Mougnekabol, J;Pratschke, J;Sauer, IM;Haep, N;
    Stem cell research
    Species: Human
    Sample Types: Embryoid Bodies
    Applications: Immunohistochemistry
  6. Generation of two induced pluripotent stem cell lines from patients with Li-Fraumeni Syndrome carrying TP53 mutation
    Authors: Sun, J;Ren, L;Canel Rivero, G;Xu, L;Ladabaum, U;C Wu, J;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  7. Generation of two induced pluripotent stem cell lines from patients with Williams syndrome
    Authors: Dai, Y;Zhu, W;Flores Banuelos, AG;Li, J;Mukherjee, S;Algaze, C;Wu, JC;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  8. KIF1A, R1457Q, and P1688L Mutations Induce Protein Abnormal Aggregation and Autophagy Impairment in iPSC-Derived Motor Neurons
    Authors: Zhao, M;Wang, J;Liu, M;Xu, Y;Huang, J;Zhang, Y;He, J;Gu, A;Liu, M;Liu, X;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  9. Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation
    Authors: Schumacher, S;Fernkorn, M;Marten, M;Chen, R;Kim, YS;Bedzhov, I;Schröter, C;
    Nature communications
    Species: Human
    Sample Types: Embryo
    Applications: Immunohistochemistry
  10. Generation of iPSC line NCHi015-A from a patient with truncus arteriosus carrying heterozygous variants in KMT2D and NOTCH1
    Authors: Wang, J;Bering, J;Alonzo, M;Ye, S;Texter, K;Garg, V;Zhao, MT;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  11. Visualisation of gene expression within the context of tissues using an X-ray computed tomography-based multimodal approach
    Authors: Kairišs, K;Sokolova, N;Zilova, L;Schlagheck, C;Reinhardt, R;Baumbach, T;Faragó, T;van de Kamp, T;Wittbrodt, J;Weinhardt, V;
    Scientific reports
    Species: Fish - Oryzias latipes (Medaka or Japanese Rice Fish)
    Sample Types: Embryo, Organoid
    Applications: Immunohistochemistry
  12. Detection of ER Stress in iPSC-Derived Neurons Carrying the p.N370S Mutation in the GBA1 Gene
    Authors: Yarkova, ES;Grigor'eva, EV;Medvedev, SP;Tarasevich, DA;Pavlova, SV;Valetdinova, KR;Minina, JM;Zakian, SM;Malakhova, AA;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  13. Distinct pathways drive anterior hypoblast specification in the implanting human embryo
    Authors: Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M;
    Nature cell biology
    Species: Human, Mouse
    Sample Types: Embryo
    Applications: Immunohistochemistry
  14. One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo
    Authors: Lin Feng, Da Li, Yao Tian, Chengshun Zhao, Yun Sun, Xiaolong Kou et al.
    Neural Regeneration Research
  15. Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C
    Authors: Daya, NM;Döring, K;Zhuge, H;Volke, L;Stab, V;Dietz, J;Athamneh, M;Roos, A;Zaehres, H;Güttsches, AK;Mavrommatis, L;Vorgerd, M;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Immunocytochemistry
  16. Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC)
    Authors: Tripathi, D;Manhas, A;Noishiki, C;Wu, D;Adkar, S;Sallam, K;Fukaya, E;Leeper, NJ;Sayed, N;
    Stem cell research
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Preclinical and dose-ranging assessment of hESC-derived dopaminergic progenitors for a clinical trial on Parkinson's disease
    Authors: Park, S;Park, CW;Eom, JH;Jo, MY;Hur, HJ;Choi, SK;Lee, JS;Nam, ST;Jo, KS;Oh, YW;Lee, J;Kim, S;Kim, DH;Park, CY;Kim, SJ;Lee, HY;Cho, MS;Kim, DS;Kim, DW;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  18. Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells
    Authors: Maimaitili, M;Chen, M;Febbraro, F;Ucuncu, E;Kelly, R;Niclis, JC;Christiansen, JR;Mermet-Joret, N;Niculescu, D;Lauritsen, J;Iannielli, A;Klæstrup, IH;Jensen, UB;Qvist, P;Nabavi, S;Broccoli, V;Nykjær, A;Romero-Ramos, M;Denham, M;
    Nature communications
    Species: Human, Rat
    Sample Types: Whole Cells, Transduced Whole Cells, Whole Tissue
    Applications: Immunohistochemistry, Immunocytochemistry
  19. Generation of two induced pluripotent stem cell lines from a 33-year-old central core disease patient with a heterozygous dominant c.14145_14156delCTACTGGGACA (p.Asn4715_Asp4718del) deletion in the RYR1 gene
    Authors: Driver, K;Vo, C;Scriba, CK;Saker, S;Larmonier, T;Malfatti, E;Romero, NB;Ravenscroft, G;Laing, NG;Taylor, RL;Clayton, JS;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells
    Authors: Tao, Y;Li, X;Dong, Q;Kong, L;Petersen, AJ;Yan, Y;Xu, K;Zima, S;Li, Y;Schmidt, DK;Ayala, M;Mathivanan, S;Sousa, AMM;Chang, Q;Zhang, SC;
    Nature biotechnology
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Flow Cytometry
  21. Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation
    Authors: Meyer, K;Lammers, NC;Bugaj, LJ;Garcia, HG;Weiner, OD;
    Nature communications
    Species: Mouse
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: ICC
  22. Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative
    Authors: Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Generation of two induced pluripotent stem cell lines from Duchenne muscular dystrophy patients
    Authors: Liu, W;Zeng, W;Kong, X;Htet, M;Yu, R;Wheeler, M;Day, JW;Wu, JC;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Flow Cytometry
  24. In-depth comparison of Anc80L65 and AAV9 retinal targeting and characterization of cross-reactivity to multiple AAV serotypes in humans
    Authors: Maura K. Schwartz, Shibi Likhite, Tatyana A. Vetter, Megan C. Baird, Vicki McGovern, Andrea Sierra Delgado et al.
    Molecular Therapy - Methods & Clinical Development
  25. A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
    Authors: Kaneyasu Nishimura, Emilía Sif Ásgrímsdóttir, Shanzheng Yang, Ernest Arenas
    STAR Protocols
  26. Generation of an induced pluripotent stem cell line (NCHi010-A) from a 6-year-old female with Down syndrome and without congenital heart disease
    Authors: Yu Y, Alonzo M, Ye S et al.
    Stem Cell Research
  27. Protocol for generating reproducible miniaturized controlled midbrain organoids
    Authors: Muwan Chen, Jonathan Christos Niclis, Mark Denham
    STAR Protocols
  28. Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells
    Authors: Kenyu Iwatsuki, Mami Oikawa, Hisato Kobayashi, Christopher A. Penfold, Makoto Sanbo, Takuya Yamamoto et al.
    Cell Reports Methods
  29. Modeling idiopathic autism in forebrain organoids reveals an imbalance of excitatory cortical neuron subtypes during early neurogenesis
    Authors: Jourdon, A;Wu, F;Mariani, J;Capauto, D;Norton, S;Tomasini, L;Amiri, A;Suvakov, M;Schreiner, JD;Jang, Y;Panda, A;Nguyen, CK;Cummings, EM;Han, G;Powell, K;Szekely, A;McPartland, JC;Pelphrey, K;Chawarska, K;Ventola, P;Abyzov, A;Vaccarino, FM;
    Nature neuroscience
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  30. Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene
    Authors: Cunningham, D;Stanberry, I;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues
    Authors: Mehmet Girgin, Nicolas Broguiere, Lorenzo Mattolini, Matthias Lutolf
    BIO-PROTOCOL
  32. The single-cell and spatial transcriptional landscape of human gastrulation and early brain development
    Authors: Zeng B, Liu Z, Lu Y et al.
    Cell stem cell
  33. Generation of two induced pluripotent stem cell lines from spinal muscular atrophy type 1 patients carrying no functional copies of SMN1 gene
    Authors: W Zeng, X Kong, C Alamana, Y Liu, J Guzman, PD Pang, JW Day, JC Wu
    Stem Cell Research, 2023-04-17;69(0):103095.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: ICC
  34. Cynomolgus monkey embryo model captures gastrulation and early pregnancy
    Authors: J Li, Q Zhu, J Cao, Y Liu, Y Lu, Y Sun, Q Li, Y Huang, S Shang, X Bian, C Li, L Zhang, Y Wang, Y Nie, J Fu, W Li, MA Mazid, Y Jiang, W Jia, X Wang, Y Sun, MA Esteban, Q Sun, F Zhou, Z Liu
    Cell Stem Cell, 2023-04-06;30(4):362-377.e7.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Embryo
    Applications: Immunohistochemistry
  35. Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G>A (p.D249N) autosomal dominant mutation in TUBB4A
    Authors: AA Almad, L Garcia, A Takanohash, A Gagne, W Yang, J Ann McGuir, D French, A Vanderver
    Stem Cell Research, 2023-03-26;69(0):103083.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  36. Establishment of TUBB3-mCherry knock-in human pluripotent stem cell line using CRISPR/Cas9 (SNUe003-A-4)
    Authors: AH Kim, HM Lee, HS Kim, J Jung, H Seol, E Choi, S Lee, Y Min Choi, JK Jun, HS Kim, J Jang
    Stem Cell Research, 2023-03-08;69(0):103064.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  37. ARF suppression by MYC but not MYCN confers increased malignancy of aggressive pediatric brain tumors
    Authors: OJ Mainwaring, H Weishaupt, M Zhao, G Rosén, A Borgenvik, L Breinschmi, AD Verbaan, S Richardson, D Thompson, SC Clifford, RM Hill, K Annusver, A Sundström, KO Holmberg, M Kasper, S Hutter, FJ Swartling
    Nature Communications, 2023-03-03;14(1):1221.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  38. Bat pluripotent stem cells reveal unusual entanglement between host and viruses
    Authors: Marion Déjosez, Arturo Marin, Graham M. Hughes, Ariadna E. Morales, Carlos Godoy-Parejo, Jonathan L. Gray et al.
    Cell
  39. Automating iPSC generation to enable autologous photoreceptor cell replacement therapy
    Authors: LR Bohrer, NE Stone, NK Mullin, AP Voigt, KR Anfinson, JL Fick, V Luangphakd, B Hittle, K Powell, GF Muschler, RF Mullins, EM Stone, BA Tucker
    Journal of Translational Medicine, 2023-02-28;21(1):161.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  40. DAPL1 prevents epithelial-mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy
    Authors: X Ma, S Han, Y Liu, Y Chen, P Li, X Liu, L Chang, YA Chen, F Chen, Q Hou, L Hou
    Cell Death & Disease, 2023-02-25;14(2):158.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. Biochemical Characteristics of iPSC-Derived Dopaminergic Neurons from N370S GBA Variant Carriers with and without Parkinson’s Disease
    Authors: Elena V. Grigor’eva, Alena E. Kopytova, Elena S. Yarkova, Sophia V. Pavlova, Diana A. Sorogina, Anastasia A. Malakhova et al.
    International Journal of Molecular Sciences
  42. Efficient generation of ETX embryoids that recapitulate the entire window of murine egg cylinder development
    Authors: C Dupont, OJM Schäffers, BF Tan, S Merzouk, EM Bindels, A Zwijsen, D Huylebroec, J Gribnau
    Science Advances, 2023-01-18;9(3):eadd2913.
    Species: Mouse
    Sample Types: Embryoids
    Applications: IHC
  43. E‐cadherin mediates apical membrane initiation site localisation during de novo polarisation of epithelial cavities
    Authors: Xuan Liang, Antonia Weberling, Chun Yuan Hii, Magdalena Zernicka‐Goetz, Clare E Buckley
    The EMBO Journal
  44. Generation of three induced pluripotent stem cell lines to model and investigate diseases affecting Hispanics
    Authors: Ian Y. Chen, Joseph Olshausen, Dilip Thomas, Celine Lai, Tracey L. McLaughlin, Joseph C. Wu
    Stem Cell Research
  45. Single-cell transcriptional and functional analysis of dopaminergic neurons in organoid-like cultures derived from human fetal midbrain
    Authors: Marcella Birtele, Petter Storm, Yogita Sharma, Janko Kajtez, Jenny Nelander Wahlestedt, Edoardo Sozzi et al.
    Development
  46. A multi-omics approach to visualize early neuronal differentiation from hESCs in 4D
    Authors: Athina Samara, Mari Spildrejorde, Ankush Sharma, Martin Falck, Magnus Leithaug, Stefania Modafferi et al.
    iScience
  47. Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Authors: E Boobalan, AH Thompson, RP Alur, DM McGaughey, L Dong, G Shih, ER Vieta-Ferr, IF Onojafe, VK Kalaskar, G Arno, AJ Lotery, B Guan, C Bender, O Memon, L Brinster, C Soleilhavo, L Panman, TC Badea, A Minella, AJ Lopez, SM Thomasy, A Moshiri, D Blain, RB Hufnagel, T Cogliati, K Bharti, BP Brooks
    Investigative Ophthalmology & Visual Science, 2022-11-01;63(12):5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  48. Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
    Authors: Aishwarya Balasankar, Shu-Yi Claire Chan, Venkata Pakala Sudheer Babu, Gary Yam, Goh Bee Tin, Shweta Singhal
    Frontiers in Medicine
  49. Mutations in BCOR, a co-repressor of CRX/OTX2, are associated with early-onset retinal degeneration
    Authors: M Langouët, C Jolicoeur, A Javed, P Mattar, MD Gearhart, SP Daiger, M Bertelsen, L Tranebjærg, ND Rendtorff, K Grønskov, C Jespersgaa, R Chen, Z Sun, H Li, N Alirezaie, J Majewski, VJ Bardwell, R Sui, RK Koenekoop, M Cayouette
    Science Advances, 2022-09-07;8(36):eabh2868.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  50. Transient upregulation of IRF1 during exit from naive pluripotency confers viral protection
    Authors: Merrit Romeike, Stephanie Spach, Marie Huber, Songjie Feng, Gintautas Vainorius, Ulrich Elling et al.
    EMBO reports
  51. Spatial profiling of early primate gastrulation in utero
    Authors: Sophie Bergmann, Christopher A. Penfold, Erin Slatery, Dylan Siriwardena, Charis Drummer, Stephen Clark et al.
    Nature
  52. Sensitivity of the Dorsal-Central Retinal Pigment Epithelium to Sodium Iodate-Induced Damage Is Associated With Overlying M-Cone Photoreceptors in Mice
    Authors: Lili Lian, Yifan Zhai, Xuejiao Wan, Linxin Chen, Zuimeng Liu, Ruona Liu et al.
    Investigative Opthalmology & Visual Science
  53. Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
    Authors: J Contreras, M Alonzo, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao
    Stem Cell Research, 2022-08-10;64(0):102893.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  54. Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)
    Authors: M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao
    Stem Cell Research, 2022-08-09;64(0):102892.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  55. Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing
    Authors: J Liu, GC Black, SJ Kimber, PI Sergouniot
    Stem Cell Research, 2022-07-30;64(0):102880.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  56. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022-07-11;63(0):102865.
    Species: Human
    Sample Types: Whole Cells, Whole Cellse
    Applications: Flow Cytometry, ICC
  57. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022-06-30;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. Nuclear Factor I in neurons, glia and during the formation of Müller glia‐derived progenitor cells in avian, porcine and primate retinas
    Authors: Heithem M. El‐Hodiri, Warren A. Campbell, Lisa E. Kelly, Evan C. Hawthorn, Maura Schwartz, Archana Jalligampala et al.
    Journal of Comparative Neurology
  59. Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting
    Authors: HM Lee, AH Kim, S Hwang, J Jung, H Seol, JJ Sung, SM Jeong, YM Choi, JK Jun, HS Kim, J Jang
    Stem Cell Research, 2022-04-04;61(0):102779.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  60. Sequential enhancer state remodelling defines human germline competence and specification
    Authors: Walfred W. C. Tang, Aracely Castillo-Venzor, Wolfram H. Gruhn, Toshihiro Kobayashi, Christopher A. Penfold, Michael D. Morgan et al.
    Nature Cell Biology
  61. Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants
    Authors: A Manhas, JWS Jahng, CD Vera, SP Shenoy, JW Knowles, JC Wu
    Stem Cell Research, 2022-03-31;61(0):102774.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  62. Efficient embryoid-based method to improve generation of optic vesicles from human induced pluripotent stem cells
    Authors: Jonathan Eintracht, Philippa Harding, Dulce Lima Cunha, Mariya Moosajee
    F1000Research
  63. Transcription factor Foxp1 is essential for the induction of choroidal neovascularization
    Authors: Meifang Yan, Junjian Li, Li Yan, Xue Li, Jie-Guang Chen
    Eye and Vision
  64. The RNA‐binding protein and stress granule component ATAXIN‐2 is expressed in mouse and human tissues associated with glaucoma pathogenesis
    Authors: Chad A. Sundberg, Monika Lakk, Sharan Paul, Karla P. P. Figueroa, Daniel R. Scoles, Stefan M. Pulst et al.
    Journal of Comparative Neurology
  65. Statistically derived geometrical landscapes capture principles of decision-making dynamics during cell fate transitions
    Authors: Meritxell Sáez, Robert Blassberg, Elena Camacho-Aguilar, Eric D. Siggia, David A. Rand, James Briscoe
    Cell Systems
  66. Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing
    Authors: P Nouri, A Zimmer, S Brüggemann, R Friedrich, R Kühn, N Prakash
    Cells, 2022-01-13;11(2):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  67. Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently
    Authors: S Touhami, F Béguier, T Yang, S Augustin, C Roubeix, F Blond, JB Conart, JA Sahel, B Bodaghi, C Delarasse, X Guillonnea, F Sennlaub
    International Journal of Molecular Sciences, 2022-01-08;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  68. Biocompatibility of Human Induced Pluripotent Stem Cell-Derived Retinal Progenitor Cell Grafts in Immunocompromised Rats
    Authors: IC Han, LR Bohrer, KN Gibson-Cor, LA Wiley, A Shrestha, BE Harman, C Jiao, EH Sohn, R Wendland, BN Allen, KS Worthingto, RF Mullins, EM Stone, BA Tucker
    Cell Transplantation, 2022-01-01;31(0):9636897221104.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  69. Chemically induced senescence in human stem cell-derived neurons promotes phenotypic presentation of neurodegeneration
    Authors: Fathi A, Mathivanan S, Kong L et al.
    Aging Cell
  70. A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants
    Authors: P Aldrin-Kir, M Åkerblom, T Cardoso, S Nolbrant, AF Adler, X Liu, A Heuer, M Davidsson, M Parmar, T Björklund
    Stem Cell Reports, 2021-12-30;17(1):159-172.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  71. Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker
    Stem Cell Research, 2021-12-23;59(0):102635.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  72. N6-methyladenosine (m 6 A) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells
    Authors: Kang-Xuan Jin, Rujuan Zuo, Konstantinos Anastassiadis, Arne Klungland, Carsten Marr, Adam Filipczyk
    Proceedings of the National Academy of Sciences
  73. Generation of three iPSC lines from dilated cardiomyopathy patients carrying a pathogenic LMNA variant
    Authors: C Lee, S Cho, C Lai, S Shenoy, R Vagelos, JC Wu
    Stem Cell Research, 2021-12-21;59(0):102638.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  74. Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs
    Authors: J Langkabel, A Horne, L Bonaguro, L Holsten, T Hesse, A Knaus, Y Riedel, M Becker, K Händler, T Elmzzahi, K Bassler, N Reusch, LH Yeghiazari, T Pecht, A Saglam, T Ulas, AC Aschenbren, F Kaiser, C Kubaczka, JL Schultze, H Schorle
    Nature Communications, 2021-12-16;12(1):7322.
    Species: Mouse
    Sample Types: Organoid
    Applications: ICC
  75. Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations
    Authors: N Belbachir, C Lai, JW Rhee, Y Zhuge, MV Perez, K Sallam, JC Wu
    Stem Cell Research, 2021-11-23;57(0):102605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  76. Gene regulatory networks controlling temporal patterning, neurogenesis, and cell-fate specification in mammalian retina.
    Authors: Pin L, Thanh H, Clayton S et al.
    Cell Rep.
  77. mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent M�ller glia
    Authors: S Lim, YJ Kim, S Park, JH Choi, Y Sung, K Nishimori, Z Kozmik, HW Lee, JW Kim
    Elife, 2021-10-22;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  78. A look into retinal organoids: methods, analytical techniques, and applications
    Authors: Afanasyeva TAV, Corral-Serrano JC, Garanto A et al.
    Cellular and Molecular Life Sciences
  79. Photoreceptor cKO of OTX2 Enhances OTX2 Intercellular Transfer in the Retina and Causes Photophobia
    Authors: Pasquale Pensieri, Annabelle Mantilleri, Damien Plassard, Takahisa Furukawa, Kenneth L. Moya, Alain Prochiantz et al.
    eNeuro
  80. Early cis-regulatory events in the formation of retinal horizontal cells
    Authors: Estie Schick, Kevin C. Gonzalez, Pooja Dutta, Kazi Hossain, Miruna G. Ghinia Ghinia Tegla, Mark M. Emerson
    Developmental Biology
  81. Highly conserved and cis-acting lncRNAs produced from paralogous regions in the center of HOXA and HOXB clusters in the endoderm lineage
    Authors: Neta Degani, Yoav Lubelsky, Rotem Ben-Tov Perry, Elena Ainbinder, Igor Ulitsky
    PLOS Genetics
  82. Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development
    Authors: L Zilova, V Weinhardt, T Tavhelidse, C Schlagheck, T Thumberger, J Wittbrodt
    Elife, 2021-07-12;10(0):.
    Species: Medaka, Zebrafish
    Sample Types: Embryo, Organoid
    Applications: IHC
  83. Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, V Thaker
    Stem Cell Research, 2021-06-21;54(0):102432.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  84. Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification
    Authors: D Meisterman, A Bruneau, S Loubersac, A Reignier, J Firmin, V François-C, S Kilens, Y Lelièvre, J Lammers, M Feyeux, P Hulin, S Nedellec, B Bretin, G Castel, N Allègre, S Covin, A Bihouée, M Soumillon, T Mikkelsen, P Barrière, C Chazaud, J Chappell, V Pasque, J Bourdon, T Fréour, L David
    Cell Stem Cell, 2021-05-17;0(0):.
    Species: Human
    Sample Types: Embryo
    Applications: IHC
  85. Naive stem cell blastocyst model captures human embryo lineage segregation
    Authors: A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo
    Cell Stem Cell, 2021-05-05;28(6):1016-1022.e4.
    Species: Human
    Sample Types: Embryo
    Applications: ICC
  86. Grafts Derived from an alpha -Synuclein Triplication Patient Mediate Functional Recovery but Develop Disease-Associated Pathology in the 6-OHDA Model of Parkinson’s Disease
    Authors: Shelby Shrigley, Fredrik Nilsson, Bengt Mattsson, Alessandro Fiorenzano, Janitha Mudannayake, Andreas Bruzelius et al.
    Journal of Parkinson's Disease
  87. Igf signaling couples retina growth with body growth by modulating progenitor cell division
    Authors: Clara Becker, Katharina Lust, Joachim Wittbrodt
    Development
  88. Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy
    Authors: L Liu, SP Shenoy, JWS Jahng, Y Liu, JW Knowles, Y Zhuge, JC Wu
    Stem Cell Research, 2021-03-11;53(0):102279.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  89. Integrin &beta1 coordinates survival and morphogenesis of the embryonic lineage upon implantation and pluripotency transition
    Authors: MA Molè, A Weberling, R Fässler, A Campbell, S Fishel, M Zernicka-G
    Cell Reports, 2021-03-09;34(10):108834.
    Species: Mouse
    Sample Types: Embryo, Whole Cells
    Applications: ICC, IHC
  90. Formative pluripotent stem cells show features of epiblast cells poised for gastrulation
    Authors: X Wang, Y Xiang, Y Yu, R Wang, Y Zhang, Q Xu, H Sun, ZA Zhao, X Jiang, X Wang, X Lu, D Qin, Y Quan, J Zhang, N Shyh-Chang, H Wang, N Jing, W Xie, L Li
    Cell Research, 2021-02-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  91. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro
    Authors: Gianluca Amadei, Kasey Y.C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan et al.
    Developmental Cell
  92. Trophectoderm mechanics direct epiblast shape upon embryo implantation
    Authors: A Weberling, M Zernicka-G
    Cell Reports, 2021-01-19;34(3):108655.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  93. Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation
    Authors: F Nilsson, P Storm, E Sozzi, D Hidalgo Gi, M Birtele, Y Sharma, M Parmar, A Fiorenzano
    Cells, 2021-01-12;10(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  94. Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate
    Authors: H De Belly, A Stubb, A Yanagida, C Labouesse, PH Jones, EK Paluch, KJ Chalut
    Cell Stem Cell, 2020-11-19;0(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: IHC
  95. A Human Induced Pluripotent Stem Cell-Derived Isogenic Model of Huntington's Disease Based on Neuronal Cells Has Several Relevant Phenotypic Abnormalities
    Authors: T Malankhano, L Suldina, E Grigor'eva, S Medvedev, J Minina, K Morozova, E Kiseleva, S Zakian, A Malakhova
    J Pers Med, 2020-11-09;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  96. Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling
    Authors: Parivash Nouri, Sebastian Götz, Benedict Rauser, Martin Irmler, Changgeng Peng, Dietrich Trümbach et al.
    Frontiers in Cell and Developmental Biology
  97. Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium
    Authors: F Jacob, SR Pather, WK Huang, F Zhang, SZH Wong, H Zhou, B Cubitt, W Fan, CZ Chen, M Xu, M Pradhan, DY Zhang, W Zheng, AG Bang, H Song, J Carlos de, GL Ming
    Cell Stem Cell, 2020-09-21;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  98. Developmental changes in the accessible chromatin, transcriptome and Ascl1-binding correlate with the loss in M�ller Glial regenerative potential
    Authors: LS VandenBosc, SG Wohl, MS Wilken, M Hooper, C Finkbeiner, K Cox, L Chipman, TA Reh
    Sci Rep, 2020-08-12;10(1):13615.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  99. Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinson�s disease patient carrying the heterozygous p.A30P mutation in SNCA
    Authors: PA Barbuti, BFR Santos, CM Dording, G Cruciani, F Massart, A Hummel, R Krüger
    Stem Cell Res, 2020-08-08;48(0):101951.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  100. Prdm1 overexpression causes a photoreceptor fate-shift in nascent, but not mature, bipolar cells
    Authors: Noah B. Goodson, Ko U. Park, Jason S. Silver, Vince A. Chiodo, William W. Hauswirth, Joseph A. Brzezinski
    Developmental Biology
  101. The 10q26 Risk Haplotype of Age-Related Macular Degeneration Aggravates Subretinal Inflammation by Impairing Monocyte Elimination
    Authors: Fanny Beguier, Michael Housset, Christophe Roubeix, Sebastien Augustin, Yvrick Zagar, Caroline Nous et al.
    Immunity
  102. Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation
    Authors: LTP Khoa, YC Tsan, F Mao, DM Kremer, P Sajjakulnu, L Zhang, B Zhou, X Tong, NV Bhanu, C Choudhary, BA Garcia, L Yin, GD Smith, TL Saunders, SL Bielas, CA Lyssiotis, Y Dou
    Cell Stem Cell, 2020-06-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  103. Basement membrane remodelling regulates mouse embryogenesis
    Authors: C Kyprianou, N Christodou, RS Hamilton, W Nahaboo, DS Boomgaard, G Amadei, I Migeotte, M Zernicka-G
    Nature, 2020-05-06;582(7811):253-258.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  104. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020-05-05;31(5):107599.
    Species: Human, Mouse
    Sample Types:
    Applications: Cell Culture, IF
  105. An ocular glymphatic clearance system removes beta -amyloid from the rodent eye
    Authors: Xiaowei Wang, Nanhong Lou, Allison Eberhardt, Yujia Yang, Peter Kusk, Qiwu Xu et al.
    Science Translational Medicine
  106. Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells
    Authors: R Kitajima, R Nakai, T Imamura, T Kameda, D Kozuka, H Hirai, H Ito, H Imai, M Imamura
    Stem Cell Res, 2020-02-28;44(0):101749.
    Species: Pan troglodytes (Chimpanzee)
    Sample Types: Whole Cells
    Applications: ICC
  107. Homeoprotein OTX1 and OTX2 involvement in rat myenteric neuron adaptation after DNBS-induced colitis
    Authors: M Bistoletti, G Micheloni, N Baranzini, A Bosi, A Conti, V Filpa, C Pirrone, G Millefanti, E Moro, A Grimaldi, R Valli, A Baj, F Crema, C Giaroni, G Porta
    PeerJ, 2020-02-13;8(0):e8442.
    Species: Rat
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  108. Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease
    Authors: CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish
    Cell Stem Cell, 2020-02-13;0(0):.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: ICC
  109. Endoplasmic Reticulum Stress Activation in Alport Syndrome Varies Between Genotype and Cell Type
    Authors: Cong Wang, Shenghui Liang, Shijia Xing, Ke Xu, Huijie Xiao, Haiyue Deng et al.
    Frontiers in Genetics
  110. Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway
    Authors: SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies
    Front Cell Neurosci, 2019-12-03;13(0):527.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  111. Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
    Authors: C Lu, NE Sanjana
    Stem Cell Res, 2019-11-01;41(0):101643.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  112. Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene
    Authors: AJ Duarte, D Ribeiro, R Santos, L Moreira, J Bragança, O Amaral
    Stem Cell Res, 2019-10-18;41(0):101595.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  113. hESC-Derived Dopaminergic Transplants Integrate into Basal Ganglia Circuitry in a Preclinical Model of Parkinson’s Disease
    Authors: Andrew F. Adler, Tiago Cardoso, Sara Nolbrant, Bengt Mattsson, Deirdre B. Hoban, Ulla Jarl et al.
    Cell Reports
  114. Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
    Authors: MT De Angelis, G Santamaria, EI Parrotta, S Scalise, M Lo Conte, S Gasparini, E Ferlazzo, U Aguglia, C Ciampi, A Sgura, G Cuda
    J. Cell. Mol. Med., 2019-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  115. Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene
    Authors: J Domingo-Pr, M Riera, V Abad-Moral, S Ruiz-Nogal, B Corcostegu, E Pomares
    Stem Cell Res, 2019-09-04;40(0):101570.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  116. Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene
    Authors: J Domingo-Pr, V Abad-Moral, M Riera, R Navarro, B Corcostegu, E Pomares
    Stem Cell Res, 2019-09-04;40(0):101569.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  117. Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors
    Authors: Diego F. Buenaventura, Adrianne Corseri, Mark M. Emerson
    Investigative Opthalmology & Visual Science
  118. Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene
    Authors: J Domingo-Pr, M Riera, A Burés-Jels, B Corcostegu, E Pomares
    Stem Cell Res, 2019-05-22;38(0):101468.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  119. Vesicular GABA Transporter Is Necessary for Transplant-Induced Critical Period Plasticity in Mouse Visual Cortex
    Authors: Rashi Priya, Benjamin Rakela, Megumi Kaneko, Julien Spatazza, Philip Larimer, Mahmood S. Hoseini et al.
    The Journal of Neuroscience
  120. Ultraviolet B-induced Otx2 expression in lens epithelial cells promotes epithelial–mesenchymal transition
    Authors: Yasuo Yoshitomi, Hiromi Osada, Haruka Satake, Masami Kojima, Hidehito Saito-Takatsuji, Takayuki Ikeda et al.
    Biology Open
  121. Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations
    Authors: M Riera, A Patel, A Burés-Jels, B Corcostegu, S Chang, E Pomares, B Corneo, JR Sparrow
    Stem Cell Res, 2019-02-13;36(0):101389.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  122. Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations
    Authors: M Riera, A Patel, B Corcostegu, S Chang, JR Sparrow, E Pomares, B Corneo
    Stem Cell Res, 2019-01-17;35(0):101385.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  123. Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene
    Authors: M Riera, A Patel, B Corcostegu, S Chang, B Corneo, JR Sparrow, E Pomares
    Stem Cell Res, 2019-01-17;35(0):101386.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  124. Photoreceptor degeneration in microphthalmia (Mitf) mice: partial rescue by pigment epithelium-derived factor
    Authors: Y Chen, J Yang, H Geng, L Li, J Li, B Cheng, X Ma, H Li, L Hou
    Dis Model Mech, 2019-01-11;12(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  125. Developmental Requirement of Homeoprotein Otx2 for Specific Habenulo-Interpeduncular Subcircuits
    Authors: N Ruiz-Reig, M Rakotobe, I Bethus, G Le Menn, HI Huditz, H Marie, T Lamonerie, F D'Autréaux
    J. Neurosci., 2018-12-28;39(6):1005-1019.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  126. Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene
    Authors: JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French
    Stem Cell Res, 2018-12-10;34(0):101361.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  127. Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation
    Authors: KE Worley, AS Chin, LQ Wan
    Stem Cells Int, 2018-12-02;2018(0):1848605.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  128. Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
    Authors: Baulier E, Garcia Diaz A, Corneo B, Farber D
    Stem Cell Res, 2018-11-28;33(0):274-277.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  129. Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation
    Authors: R Diacou, Y Zhao, D Zheng, A Cvekl, W Liu
    Cell Rep, 2018-11-27;25(9):2510-2523.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  130. Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development
    Authors: Thorsten Boroviak, Giuliano G. Stirparo, Sabine Dietmann, Irene Hernando-Herraez, Hisham Mohammed, Wolf Reik et al.
    Development
  131. Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells
    Authors: Diego F. Buenaventura, Miruna G. Ghinia Ghinia-Tegla, Mark M. Emerson
    Developmental Biology
  132. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018-10-19;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  133. Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming
    Authors: AD Senft, I Costello, HW King, AW Mould, EK Bikoff, EJ Robertson
    Cell Rep, 2018-08-21;24(8):1977-1985.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  134. Human axial progenitors generate trunk neural crest cells in vitro
    Authors: Thomas JR Frith, Ilaria Granata, Matthew Wind, Erin Stout, Oliver Thompson, Katrin Neumann et al.
    eLife
  135. Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL
    Authors: AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French
    Stem Cell Res, 2018-07-05;31(0):157-160.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  136. In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells
    Authors: SE Harrison, B Sozen, M Zernicka-G
    Nat Protoc, 2018-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  137. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells
    Authors: JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim
    Exp. Mol. Med., 2018-04-13;50(4):24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  138. Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning
    Authors: Sophie M Morgani, Jakob J Metzger, Jennifer Nichols, Eric D Siggia, Anna-Katerina Hadjantonakis
    eLife
  139. Prdm13 is required for Ebf3+ amacrine cell formation in the retina
    Authors: Noah B. Goodson, Jhenya Nahreini, Grace Randazzo, Ana Uruena, Jane E. Johnson, Joseph A. Brzezinski
    Developmental Biology
  140. A new genetically engineered mouse model of choroid plexus carcinoma
    Authors: S El Nagar, F Zindy, C Moens, L Martin, D Plassard, MF Roussel, T Lamonerie, N Billon
    Biochem. Biophys. Res. Commun., 2018-01-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  141. Tau Deficiency Down-Regulated Transcription Factor Orthodenticle Homeobox 2 Expression in the Dopaminergic Neurons in Ventral Tegmental Area and Caused No Obvious Motor Deficits in Mice
    Authors: X Tang, L Jiao, M Zheng, Y Yan, Q Nie, T Wu, X Wan, G Zhang, Y Li, S Wu, B Jiang, H Cai, P Xu, J Duan, X Lin
    Neuroscience, 2018-01-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  142. Otx2-Genetically Modified Retinal Pigment Epithelial Cells Rescue Photoreceptors after Transplantation
    Authors: Christo Kole, Laurence Klipfel, Ying Yang, Vanessa Ferracane, Frederic Blond, Sacha Reichman et al.
    Molecular Therapy
  143. Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of M�ller Glia-Derived Progenitor Cells in the Avian Retina
    Authors: L Todd, L Suarez, C Quinn, AJ Fischer
    Stem Cells, 2017-11-27;0(0):.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  144. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling
    Authors: Xin Huang, Sophie Balmer, Fan Yang, Miguel Fidalgo, Dan Li, Diana Guallar et al.
    eLife
  145. Small molecule Photoregulin3 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa
    Authors: PA Nakamura, AA Shimchuk, S Tang, Z Wang, K DeGolier, S Ding, TA Reh
    Elife, 2017-11-17;6(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  146. Genome-Wide Target Analyses of Otx2 Homeoprotein in Postnatal Cortex
    Authors: Akiko Sakai, Ryuichiro Nakato, Yiwei Ling, Xubin Hou, Norikazu Hara, Tomoya Iijima et al.
    Frontiers in Neuroscience
  147. Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c–SIRT2 axis
    Authors: Young Cha, Min-Joon Han, Hyuk-Jin Cha, Janet Zoldan, Alison Burkart, Jin Hyuk Jung et al.
    Nature Cell Biology
  148. Retinal pigment epithelial integrity is compromised in the developing albino mouse retina
    Authors: Lena Iwai-Takekoshi, Anna Ramos, Ari Schaler, Samuel Weinreb, Richard Blazeski, Carol Mason
    Journal of Comparative Neurology
  149. Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-?
    Aging Cell, 2016-09-22;0(0):.
    Species: Human, Porcine
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC-Fr, Western Blot
  150. OTX2 Transcription Factor Controls Regional Patterning Within The Medial Ganglionic Eminence (MGE) And Regional Identity Of The Septum
    Authors: Renée V. Hoch, Susan Lindtner, James D. Price, John L.R. Rubenstein
    Cell Reports
  151. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Authors: Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel
    Nat Commun, 2015-03-23;6(0):6500.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  152. SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice.
    Authors: Wu Q, Fukuda K, Weinstein M, Graff J, Saga Y
    Development, 2015-02-01;142(3):575-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  153. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Authors: Zou M, Luo H, Xiang M
    Dev Dyn, 2014-09-16;244(1):86-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  154. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
    Authors: Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio
    Development, 2013-12-11;141(2):377-88.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  155. Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages.
    Authors: Stott, Simon R, Metzakopian, Emmanoui, Lin, Wei, Kaestner, Klaus H, Hen, Rene, Ang, Siew-Lan
    J Neurosci, 2013-05-01;33(18):8022-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  156. Trophic factors differentiate dopamine neurons vulnerable to Parkinson's disease
    Authors: Stefanie Reyes, Yuhong Fu, Kay L. Double, Veronica Cottam, Lachlan H. Thompson, Deniz Kirik et al.
    Neurobiology of Aging
  157. An essential role for RAX homeoprotein and NOTCH-HES signaling in Otx2 expression in embryonic retinal photoreceptor cell fate determination
    Authors: Yuki Muranishi, Koji Terada, Tatsuya Inoue, Kimiko Katoh, Toshinori Tsujii, Rikako Sanuki et al.
    The Journal of Neuroscience
  158. The Timing of Sonic Hedgehog and Gli1 Expression Segregates Midbrain Dopamine Neurons
    Authors: Lindsay Hayes, Zhiwei Zhang, Paul Albert, Mark Zervas, Sohyun Ahn
    The Journal of Comparative Neurology
  159. Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours.
    Authors: Rijlaarsdam MA, van Herk HA, Gillis AJ
    Br. J. Cancer, 2011-08-16;105(6):854-63.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  160. Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
    Authors: Di Salvio M, Di Giovannantonio LG, Acampora D
    Nat. Neurosci., 2010-11-07;13(12):1481-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  161. Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs
    Authors: Bao-Yang Hu, Su-Chun Zhang
    Cellular Programming and Reprogramming
  162. Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.
    Authors: Zeng H, Guo M, Martins-Taylor K
    PLoS ONE, 2010-07-29;5(7):e11853.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  163. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009-01-20;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  164. Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
    Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
    Stem Cells, 2007-03-01;25(6):1511-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  165. Generation of highly enriched populations of optic vesicle-like retinal cells from human pluripotent stem cells.
    Authors: Ohlemacher SK, Iglesias CL, Sridhar A et al.
    Curr Protoc Stem Cell Biol
  166. Establishment of NCHi009-A, an iPSC line from a patient with hypoplastic left heart syndrome (HLHS) carrying a heterozygous NOTCH1 mutation
    Authors: S Adhicary, S Ye, H Lin, K Texter, V Garg, MT Zhao
    Stem Cell Research, 2022-12-31;66(0):103013.
  167. Genetic reprogramming of human amniotic cells with episomal vectors: neural rosettes as sentinels in candidate selection for validation assays.
    Authors: Wilson Patricia G, Payne Tiffany.
    PeerJ.
  168. Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
    Authors: H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride
    Stem Cell Research, 2022-10-31;65(0):102958.
  169. An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
    Authors: Gantner C, Cota-Coronado A, Thompson L, Parish C
    STAR Protoc
  170. Induced hepatic stem cells are suitable for human hepatocyte production
    Authors: Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al.
    iScience
  171. Transcriptional repression of Plxnc1 by Lmx1a and Lmx1b directs topographic dopaminergic circuit formation
    Authors: A Chabrat, G Brisson, H Doucet-Bea, C Salesse, M Schaan Pro, A Dovonou, C Akitegetse, J Charest, S Lemstra, D Côté, RJ Pasterkamp, MI Abrudan, E Metzakopia, SL Ang, M Lévesque
    Nat Commun, 2017-10-16;8(1):933.
  172. Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients
    Authors: PH Zushin, Y Zhou, A Li, EA Ashley, MT Wheeler, JC Wu
    Stem Cell Research, 2023-01-05;67(0):103019.
  173. Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization
    Authors: M Shigyo, Y Kobayashi, O Platoshyn, S Marsala, T Kato, N Takamura, K Yoshida, A Kishino, M Bravo-Hern, S Juhas, J Juhasova, H Studenovsk, V Proks, JD Ciacci, M Marsala
    Cell Transplantation, 2023-01-01;32(0):9636897231163.

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Human Otx2 Antibody
By Sébastien Brot on 09/21/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: iPS2 human induced pluripotent stem cells Species: Human
Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Anonymous on 07/12/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain,neuron cells Species: Human
Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Anonymous on 06/29/2021
Application: IHC Sample Tested: Adult brain Species: Human
Immunohistochemistry Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Anonymous on 12/21/2020
Application: IHC Sample Tested: Fetal brain Species: Human
Immunohistochemistry Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Alina Filatova on 10/04/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Embryonic brain Species: Mouse

This antibody works very well at 1:1000 in immunofluorescence staining of mouse paraffin-embedded sections. Epitope retrieval: Citrate buffer.

Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Anonymous on 08/22/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Undifferentiated neural progenitor cells Species: Human

ICC on hPSC derived neurl progenitors

Immunocytochemistry/Immunofluorescence Human Otx2 Antibody AF1979
Human Otx2 Antibody
By Isis Trujillo on 06/29/2016
Application: IHC Sample Tested: Embryonic retina Species: Mouse

Otx2 staining on frozen mouse embryonic day 17 eyes sections using AF1979 The sections were blocked using 0.01% Triton-X, 2% donkey serum in 1X PBS 1 hour at room temperature. OTX2 was diluted 1:500 using blocking buffer and incubated overnight at 4°C. The secondary antibody used was donkey ant goat conjugated to Alexa Fluor 488 (1:2000).