Human Park7/DJ-1 Antibody

Detection of Human Park7/DJ‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Park7/DJ-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Park7/DJ‑1 in HeLa Human Cell Line. Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Park7/DJ‑1 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ-1 at approximately 28 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human Park7/DJ‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Park7/DJ‑1 in Human Brain Hypothalamus. Park7/DJ‑1 was detected in immersion fixed paraffin-embedded sections of Human Brain Hypothalamus using Goat Anti-Human Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.