Human/Rat GDNF Antibody

Catalog #: AB-212-NA
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AB-212-NA

Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat GDNF Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat GDNF in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human GDNF (Catalog # 212-GD)
Recombinant Rat GDNF (Catalog # 512-GF)

Neutralization

Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 7.5-30 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AB-212-NA). The ND₅₀is typically 7.5-30 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name

Glial Cell line-derived Growth Factor

Entrez Gene IDs

2668 (Human); 25453 (Rat)

Alternate Names

Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Rat GDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease
Authors: CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish
Cell Stem Cell, 2020-02-13;0(0):.
Species: Mouse, Rat
Sample Types: Whole Tissue
Applications: IHC
Gdnf, Neurturin and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute To Inflammatory Bone Pain
Authors: S Nencini, M Ringuet, DH Kim, C Greenhill, JJ Ivanusic
J. Neurosci., 2018-04-30;0(0):.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization
Developing therapeutically more efficient Neurturin variants for treatment of Parkinson's disease
Authors: Pia Runeberg-Roos, Elisa Piccinini, Anna-Maija Penttinen, Kert Mätlik, Hanna Heikkinen, Satu Kuure et al.
Neurobiology of Disease
Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
Nature, 2016-07-13;535(7612):440-3.
Species: Mouse
Sample Types: Whole Cells
Applications: Blocking