Human SLAM/CD150 Antibody

Catalog #: MAB1642
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1642
MAB1642-SP

Detection of Human SLAM/CD150 by Western Blot.

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All SLAM/CD150 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLAM/CD150 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLAM/CD150 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 542301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SLAM/CD150
Ala21-Pro237
Accession # Q13291

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SLAM/CD150 DuoSet ELISA Kit (Catalog # DY164) for convenient development of a sandwich ELISA.

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SLAM/CD150 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 10 ng/mL PMA and 200 ng/mL ionomycin for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLAM/CD150 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

ELISA

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Human SLAM/CD150 ELISA Standard Curve. Recombinant Human SLAM/CD150 protein was serially diluted 2-fold and captured by Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

Signaling lymphocytic activation molecule (SLAM, SLAMF1; CD150) was the first identified of a family of type I transmembrane (TM) lymphocyte activating receptors. SLAM homotypic adhesion bidirectionally stimulates T and B cells. SLAM is also expressed by hematopoietic stem cells, dendritic cells and platelets and is a T cell measles virus receptor. The 70 kDa glycoprotein contains a 216 amino acid (aa) extracellular domain (ECD) with one C2 type and one V type Ig-like domain, a 20 aa TM sequence and a 76 aa SH2-binding cytoplasmic domain. One splice variant has a shorter cytoplasmic tail and another lacks the TM sequence and is secreted. Human and mouse SLAM ECD share 60% aa identity.

Long Name

Signaling Lymphocytic Activation Molecule

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Alternate Names

CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human SLAM/CD150 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2
Authors: L von Wenser, C Schulthei beta, S Bolz, S Schliffke, D Simnica, E Willscher, H Gerull, G Wolters-Ei, K Riecken, B Fehse, M Altfeld, P Nollau, M Binder
Leukemia, 2020-08-21;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation, Immunoprecipitation
Chondroitin 6-Sulfation Regulates Perineuronal Net Formation by Controlling the Stability of Aggrecan
Authors: Shinji Miyata, Hiroshi Kitagawa
Neural Plasticity