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Human Survivin Antibody

Catalog #: MAB886
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB886
MAB886-SP
Detection of Survivin in Human PBMC lymphocytes by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Survivin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Survivin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 91630
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Survivin
Met1-Asp142
Accession # O15392
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Survivin antibody in Human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of Survivin in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cells were labeled with Human Survivin Monoclonal Antibody (Catalog # MAB886, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Survivin

Survivin is a member of the inhibitor of apoptosis (IAP) family and can inhibit apoptosis induced by a variety of factors. It is expressed in most human cancers but not in normal adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.

Entrez Gene IDs
332 (Human)
Alternate Names
API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin

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Citations for Human Survivin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Survivin is not a promising serological maker for the diagnosis of hepatocellular carcinoma.
    Authors: Jia X, Gao Y, Zhai D et al.
    Oncol Lett
  2. Monitoring the in vivo siRNA release from lipid nanoparticles based on the fluorescence resonance energy transfer principle
    Authors: L Sun, J Zhang, JE Zhou, J Wang, Z Wang, S Luo, Y Wang, S Zhu, F Yang, J Tang, W Lu, Y Wang, L Yu, Z Yan
    Asian journal of pharmaceutical sciences, 2022-12-07;18(1):100769.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Single-cell glycomics analysis by CyTOF-Lec reveals glycan features defining cells differentially susceptible to HIV
    Authors: T Ma, M McGregor, L Giron, G Xie, AF George, M Abdel-Mohs, NR Roan
    Elife, 2022-07-05;11(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy
    Authors: F Colomb, LB Giron, L Kuri-Cerva, OS Adeniji, T Ma, H Dweep, E Battivelli, E Verdin, CS Palmer, H Tateno, AV Kossenkov, NR Roan, MR Betts, M Abdel-Mohs
    Cell Rep, 2020-08-04;32(5):107991.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof, Flow Cytometry
  5. Phase I clinical trial of a novel autologous modified-DC vaccine in patients with resected NSCLC
    Authors: C Ge, R Li, H Song, T Geng, J Yang, Q Tan, L Song, Y Wang, Y Xue, Z Li, S Dong, Z Zhang, N Zhang, J Guo, L Hua, S Chen, X Song
    BMC Cancer, 2017-12-21;17(1):884.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Generation of allo-restricted peptide-specific T cells using RNA-pulsed dendritic cells: A three phase experimental procedure.
    Authors: Wilde S, Geiger C, Milosevic S, Mosetter B, Eichenlaub S, Schendel D
    Oncoimmunology, 2012-03-01;1(2):129-140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.
    Authors: Schneider P, Costa O, Legrand E
    Leuk. Res., 2009-09-12;34(1):24-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human Survivin Antibody
By Abby Tyler on 11/07/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The antibody MAB886 was used as the capture in a sandwich ELISA along with AF6471 as detection. The ELISA worked very well. Healthy human serum and plasma samples were not detectable although some disease state samples are detectable.

ELISA Human Survivin Antibody MAB886