Human TFF2 Antibody

Catalog #: AF4077 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4077
AF4077-SP

Detection of Human TFF2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human TFF3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TFF2
Glu24-Tyr129
Accession # Q03403

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TFF2 by Western Blot. Western blot shows lysates of human stomach tissue, human stomach cancer tissue, human breast tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TFF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TFF2 at approximately 19-21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF2

Trefoil factor 2 (TFF2), also known as spasmolytic polypeptide (Sp) is a 20 kDa secreted trefoil factor family protein. It is a protease-resistant glycoprotein primarily produced by mucous-secreting cells of the stomach and duodenum and is upregulated in response to inflammation. TFF2 interacts with mucin and other TFF2-binding proteins to protect and repair mucosal epithelium. TFF2 is also expressed by macrophages and lymphocytes and may have a role in modulating immune system responses. The 106 residue mature human TFF2 contains two trefoil domains (aa 36-72 and aa 80-121) and one N-linked glycosylation site. Human TFF2 shares 71% and 87% aa sequence identity with canine and mouse TFF2, respectively.

Long Name

Trefoil Factor 2

Entrez Gene IDs

7032 (Human); 21785 (Mouse); 116592 (Rat)

Alternate Names

SML1; SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1; SP; Spasmolysin; Spasmolytic Polypeptide; TFF2; trefoil factor 2