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Human ZBTB38 Antibody

Catalog #: AF5784
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5784
AF5784-SP
Detection of Human ZBTB38 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZBTB38 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human ZBTB38 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ZBTB38
Met1-Tyr232
Accession # Q8NAP3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ZBTB38 antibody by Western Blot. View Larger

Detection of Human ZBTB38 by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human ZBTB38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5784) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ZBTB38 at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZBTB38

ZBTB38 (Zinc finger and BTB domain-containing protein 38; also CIBZ and Zenon) is an early member of a family of Kaiso-like DNA binding proteins. Although its predicted MW is 134 kDa, it runs anomalously in SDS-PAGE at 175 kDa. ZBTB38 binds to singly methylated CpG (cytosine-phosphate-guanine) motifs. It is considered a transcriptional repressor that either homodimerizes, or heterodimerizes with ZBTB4, and subsequently interacts with either CtBP-1 or -2 to mediate repression. Human ZBTB38 is 1195 amino acids (aa) in length. It contains a BTB domain (aa 33‑100), an RD2 region (aa 158‑341) and ten C2H2-type zinc finger domains (aa 342‑1147). There is one potential alternate start site at Met595. Over aa 1‑232, human ZBTB38 shares 96% aa identity with mouse ZBTB38.

Long Name
Zinc Finger and BTB Domain-Containing Protein 38
Entrez Gene IDs
253461 (Human); 245007 (Mouse); 315936 (Rat)
Alternate Names
CIBZ; FLJ22332; FLJ31131; FLJ35036; STQTL10; ZBTB38; zinc finger and BTB domain containing 38; zinc finger and BTB domain-containing protein 38; ZNF921

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Citation for Human ZBTB38 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cullin E3 ligase activity is required for myoblast differentiation
    Authors: J Blondelle, P Shapiro, AA Domenighet, S Lange
    J. Mol. Biol, 2017-02-24;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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