Mouse c-Rel Antibody

Name: Mouse c-Rel Antibody
Brand: R&D Systems
SKU: AF2699
Rating: 5 (1 reviews)

Catalog #: AF2699
3 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2699
AF2699-SP

Detection of Mouse c-Rel by Western Blot.

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Product Details

Citations (3)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse c-Rel Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse c-Rel in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse c-Rel
Met1-Ile588
Accession # NP_033070

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

A20 mouse B cell lymphoma cell line and CH-1 mouse B cell lymphoma cell line

Simple Western

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse c-Rel by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Rel at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse c‑Rel by Simple Western^TM. Simple Western lane view shows lysates of CH-1 mouse B cell lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c-Rel at approximately 71 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Rel

c-Rel/Rel, the cellular counterpart of the v-Rel oncogene of the avian reticuloendotheliosis retrovirus, is a 69 kDa class II member of the Rel/NK-kappa B family of transcription factors. All Rel family members contain a RHD (Rel homology domain) that is involved in dimerization, DNA and I kappa B binding, and nuclear localization. Class II members contain an additional C-terminal transcriptional activation segment. Rel both homodimerizes and heterodimerizes with multiple family members. Following dimerization, Rel complexes initiate transcription by acting on decameric DNA motifs termed kappa B binding sites. The important role of c-Rel in B-cell development, growth, and survival is well documented. c-Rel is also involved in responses to auto-antigens, allo-antigens, allergens and pathogens and may contribute to the development of certain human lymphoid cell cancers.

Long Name

Cellular Repressor of E1A-stimulated Genes

Entrez Gene IDs

5966 (Human); 19696 (Mouse)

Alternate Names

C-Rel proto-oncogene protein; cRel; c-Rel; I-Rel; oncogene REL, avian reticuloendotheliosis; proto-oncogene c-Rel; REL; v-rel avian reticuloendotheliosis viral oncogene homolog; v-rel reticuloendotheliosis viral oncogene homolog (avian)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse c-Rel Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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NF-?B c-Rel Is a Potential Therapeutic Target for Acute Corneal Transplant Rejection
Authors: Zheng, Q;Liu, R;Jiang, B;Sun, J;Wang, T;Ruan, Q;
Investigative ophthalmology & visual science
Species: Mouse
Sample Types: Protein
Applications: Western Blot
c-Rel gain in B cells drives germinal center reactions and autoantibody production
Authors: M Kober-Hass, H Oh-Strau beta, D Kumar, V Soberón, C Diehl, M Lech, T Engleitner, E Katab, V Fernandez, G Piontek, H Li, B Menze, C Ziegenhain, W Enard, R Rad, JP Böttcher, HJ Anders, M Rudelius, M Schmidt-Su
J. Clin. Invest., 2020-06-01;0(0):.
Species: Mouse
Sample Types: Cell Culture Lysates, Whole Tissue
Applications: IHC, Western Blot
TNFR-Associated Factor 2 Deficiency in B Lymphocytes Predisposes to Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma in Mice.
Authors: Perez-Chacon G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM
J. Immunol., 2012-06-18;189(2):1053-61.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot