Mouse CD99 Antibody

Catalog #: AF3905
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3905
AF3905-SP

Detection of CD99 in Mouse Splenocytes by Flow Cytometry.

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All CD99 Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD99 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD99 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD99
Gly25-Leu138
Accession # Q8VCN6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD99

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD99 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3905, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (Catalog #NL002).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD99

CD99 (also known as PILR-L and MIC2) is a 125 amino acid, 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse CD99 protein used as immunogen is 45% identical to the human counterpart.

Entrez Gene IDs

4267 (Human); 673094 (Mouse); 652929 (Rat)

Alternate Names

12E7; CD99 antigenY homolog; CD99 molecule; CD99; E2 antigen; HBA71; MIC2 (monoclonal 12E7); MIC2; MIC2Y; MSK5X; pilr-1; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse CD99 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Targeting Tumor Vascular CD99 Inhibits Tumor Growth
Authors: Elisabeth J. M. Huijbers, Inge M. van der Werf, Lisette D. Faber, Lena D. Sialino, Pia van der Laan, Hanna A. Holland et al.
Frontiers in Immunology
Neurotrophic Factor Secretion and Neural Differentiation Potential of Multilineage-differentiating Stress-enduring (Muse) Cells Derived from Mouse Adipose Tissue
Authors: Y Nitobe, T Nagaoki, G Kumagai, A Sasaki, X Liu, T Fujita, T Fukutoku, K Wada, T Tanaka, H Kudo, T Asari, KI Furukawa, Y Ishibashi
Cell Transplant, 2019-07-15;0(0):9636897198638.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin ? and promotes transendothelial cell migration
Authors: Tillmann Bedau
FASEB J, 2016-12-21;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
Evolutionarily conserved paired immunoglobulin-like receptor alpha (PILRalpha) domain mediates its interaction with diverse sialylated ligands.
Authors: Sun Y, Senger K, Baginski TK, Mazloom A, Chinn Y, Pantua H, Hamidzadeh K, Ramani SR, Luis E, Tom I, Sebrell A, Quinones G, Ma Y, Mukhyala K, Sai T, Ding J, Haley B, Shadnia H, Kapadia SB, Gonzalez LC, Hass PE, Zarrin AA
J. Biol. Chem., 2012-03-06;287(19):15837-50.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry