Mouse FGF-21 Antibody Summary
Tyr30-Ser210
Accession # Q9JJN1
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse FGF‑21 by Western Blot. Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse FGF-21 by Western Blot Increased cellular stress response in muscle of DTG mice.(A) Gene expression analysis of FGF21 in Quadriceps muscle by quantitative RT-PCR (n = 8 per group) and (B) FGF21 plasma concentration of 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice (n = 9–10 per group). (B) Representative western blots of proteins involved in integrated stress response and FGF21 induction and (C) of cellular stress response markers HSP25 and HSP70 in Quadriceps muscle from 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice, Mitofusin-2 (MFN2) was used as a loading control (n = 2 out of 6–8 analyzed per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0094689), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Mouse FGF-21 Antibody by Western Blot Increased cellular stress response in muscle of DTG mice.(A) Gene expression analysis of FGF21 in Quadriceps muscle by quantitative RT-PCR (n = 8 per group) and (B) FGF21 plasma concentration of 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice (n = 9–10 per group). (B) Representative western blots of proteins involved in integrated stress response and FGF21 induction and (C) of cellular stress response markers HSP25 and HSP70 in Quadriceps muscle from 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice, Mitofusin-2 (MFN2) was used as a loading control (n = 2 out of 6–8 analyzed per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24732703), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-21
Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.
Product Datasheets
Citations for Mouse FGF-21 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function
Authors: Timper K, Del Rio-Martin A, Cremer AL et al.
Cell Metab.
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FGF21 is required for the metabolic benefits of IKK epsilon /TBK1 inhibition
Authors: Shannon M. Reilly, Mohammad Abu-Odeh, Magdalene Ameka, Julia H. DeLuca, Meghan C. Naber, Benyamin Dadpey et al.
Journal of Clinical Investigation
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Suppressive Effect of Autocrine FGF21 on Autophagy-Deficient Hepatic Tumorigenesis
Authors: Jinyoung Kim, Soyeon Lee, Myung-Shik Lee
Frontiers in Oncology
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An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction
Authors: JY Sun, LJ Du, XR Shi, YY Zhang, Y Liu, YL Wang, BY Chen, T Liu, H Zhu, Y Liu, CC Ruan, Z Gan, H Ying, Z Yin, PJ Gao, X Yan, RG Li, SZ Duan
Science Advances, 2023-04-05;9(14):eade4110.
Species: Mouse
Sample Types: Cell Lysate
Applications: Western Blot -
SMP30-mediated synthesis of vitamin C activates the liver PPARalpha/FGF21 axis to regulate thermogenesis in mice
Authors: B Lee, HJ An, DH Kim, MK Lee, HH Jeong, KW Chung, Y Go, AY Seo, IY Kim, JK Seong, BP Yu, J Lee, E Im, IK Lee, MS Lee, KI Yamada, HY Chung
Experimental & Molecular Medicine, 2022-11-25;0(0):.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes Obesity-Induced Insulin Resistance
Authors: SM Turpin-Nol, P Hammerschm, W Chen, A Jais, K Timper, M Awazawa, S Brodesser, JC Brüning
Cell Rep, 2019-01-02;26(1):1-10.e7.
Species: Mouse
Sample Types: In Vivo, Tissue Homogenates
Applications: Neutralization, Western Blot -
Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action
EBioMedicine, 2016-12-24;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Capture) -
Activation of AMPKalpha2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity.
Authors: Ost M, Werner F, Dokas J, Klaus S, Voigt A
PLoS ONE, 2014-04-14;9(4):e94689.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Silencing of the Fibroblast growth factor 21 gene is an underlying cause of acinar cell injury in mice lacking MIST1.
Authors: Johnson, Charis L, Mehmood, Rashid, Laing, Scott W, Stepniak, Camilla, Kharitonenkov, Alexei, Pin, Christop
Am J Physiol Endocrinol Metab, 2014-02-18;306(8):E916-28.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21.
Authors: Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB
J. Biol. Chem., 2012-06-01;287(30):25123-38.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
BetaKlotho is required for metabolic activity of fibroblast growth factor 21.
Authors: Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M
Proc. Natl. Acad. Sci. U.S.A., 2007-04-23;104(18):7432-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Fibroblast Growth Factor 21 (FGF21) Protects against High Fat Diet Induced Inflammation and Islet Hyperplasia in Pancreas
Authors: Garima Singhal, ffolliott Martin Fisher, Melissa J. Chee, Tze Guan Tan, Abdelfattah El Ouaamari, Andrew C. Adams et al.
PLOS ONE
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Evaluation of markers of beige adipocytes in white adipose tissue of the mouse
Authors: Rolando A. Garcia, James N. Roemmich, Kate J. Claycombe
Nutrition & Metabolism
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Mitochondrial myopathy induces a starvation-like response
Authors: Henna Tyynismaa, Christopher J. Carroll, Nuno Raimundo, Sofia Ahola-Erkkilä, Tina Wenz, Heini Ruhanen et al.
Human Molecular Genetics
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I didn't see the band in mouse spleen tissue.
